5G (6 GHz) Radyofrekans Elektromanyetik Alanın Sıçan Kan Hücrelerinde Canlılık, Apopitotik ve Nekrotik Hücre Oranına Etkisinin Araştırılması

Year 2023, Volume: 32 Issue: 1, 16 - 25, 31.03.2023

Yasin Karamazı Mustafa Emre Salih Çetiner Çağatay Aydın Gülsevinç Aksoy Hülya Binokay Toygar Emre

https://doi.org/10.17827/aktd.1221723

Cited By: 1

Abstract

Hayatın çeşitli alanlarında radyofrekans elektromanyetik alanlara (RF-EMA) maruziyet giderek artmaktadır. Çalışmamızda, 5G (6 GHz, 0.08 W/kg SAR) RF-EMA’nın sıçan kan hücrelerinde canlılık, apoptotik (erken, geç) ve nekrotik oranlarına etkilerini araştırdık.
Çalışmamızda, ağırlıkları 250-300g arasında değişen 10 adet Wistar Albino türü erişkin erkek sıçan kullanıldı. RF-EMA uygulama öncesi tüm sıçanlardan kardiyak ponksiyon yöntemi ile kan alınarak (2cc) kontrol grubu olarak seçildi. Aynı sıçanlar özel uygulama kafesine yerleştirilerek 6 hafta boyunca 4 saat/gün 6 GHz RF-EMA’ya maruz bırakıldı. RF-EMA uygulama sonrası aynı sıçanlardan 2.kez kan alımı (2cc) yapılarak radyofrekans radyasyon (RFR) grubu olarak seçildi. Sıçan kan hücrelerinde canlı, erken/geç apopitotik ve nekrotik hücre yüzde oranları anneksin-V kiti kullanılarak flow sitometrik yöntemle analiz edildi.
RFR grubunda canlı hücrelerin % oranı, kontrol grubuna kıyasla artış gösterirken, erken apopitotik ve nekrotik hücrelerin % oranları azaldığı ve iki grup arasındaki farklılıkların istatistiksel olarak anlamlı olduğu tespit edildi (p<0.05). Ancak, geç apopitotik hücrelerin % oranları gruplar arasında istatistiksel olarak kayda değer herhangi bir değişiklik tespit edilemedi.
Sonuç olarak 6 GHz RF-EMA, kan hücre canlılığı, apopitotik ve nekrotik oranlar üzerine önemli etkilere sahipti. Kan hücrelerinde canlılık oranını desteklerken, apopitotik ve nekrotik oranlarını baskılamıştır.

Keywords

Canlılık, apopitotik, nekrotik

Supporting Institution

Çukurova Üniversitesi Bilimsel Araştırmalar Projesi (ÇUBAP)

Project Number

TDK-2021-13488

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