Hyalomma marginatum (Koch, 1844) (Acari: İxodidae)’un Ovaryumunun Histolojik Yapısı

Year 2021, Volume: 21 Issue: 1, 25 - 34, 24.02.2021

Elif Yaman , Nadim Yılmazer

https://doi.org/10.35414/akufemubid.708448

Abstract

Hyalomma marginatum’un ovaryumu ve ovogenez süreci ilk kez bu çalışmada ışık mikroskobu düzeyinde histolojik ve histokimyasal olarak incelenmiştir. Ovaryum nurse ve folikül hücrelerinden yoksundur ve bu nedenle panoistik olarak tanımlanmıştır. İçi boş tübül biçimindeki ovaryumun duvarı tek sıra epitel hücrelerinden ve gelişmekte olan ovositlerden oluşur. Ovositler bir pedisel ile hemosöle bakacak şekilde ovaryum duvarına bağlıdır. Sitoplazmanın görünüşü, germinal vesikülün varlığı, yumurta sarısı granüllerinin bileşimi ve koryonun oluşumuna göre gelişmekte olan ovositler sınıflandırılmış ve dört evre tanımlanmıştır. Olgun ovositler küresele yakın eliptik şekilli olup, en büyük ovosit 315x295 µm çapındadır. Protein ve karbonhidratların varlığını gösteren boyama teknikleri bu kene türünde, yumurta sarısı granüllerinde hem proteinlerin hem de karbonhidratların bulunduğunu, vitellogenezde karbonhidratların proteinlerden önce sentezlendiğini ve koryonda karbonhidratların baskın olduğunu göstermiştir. Çok yaygın olmasa da ovaryumda ovosit yozlaşması gözlenmiştir.

Keywords

Hyalomma marginatum, Ovaryum, Ovogenez, Histoloji, Histokimya

Thanks

Kene örneklerimizi toplayan ve teşhisini yapan bölümümüz öğretim üyesi Doç. Dr. Sırrı Kar’a teşekkür ederiz.

Histological Structure of the Ovary of Hyalomma marginatum (Koch, 1844) (Acari: Ixodidae)

Abstract

With this study, the ovary and oogenesis in Hyalomma marginatum were histologically and histochemically examined at light microscope level for the first time. The ovary lacks nurse and follicular cells, thus defined as panoistic type. Wall of the ovary, a hollow tubular structure, is composed of a layer of epithelial cells and developing oocytes. Facing hemocoel, oocytes are attached to the ovarian wall through a pedicel. The developing oocytes were classified according to cytoplasm appearance, presence of germinal vesicle, constitution of yolk granules, and formation of chorion, and accordingly four developmental stages were described. Mature oocytes were oval to rounded in shape, the largest one being 315x295 µm in diameter. Staining techniques revealing the presence of proteins and carbohydrates indicate in this tick species that yolk granules contain both proteins and carbohydrates, that carbohydrates are first to be synthesized prior to proteins during vitellogenesis, and that carbohydrates predominate in chorion. Oocyte degeneration was observed although it was not so common in the ovary.

Keywords

Hyalomma marginatum, Ovary, Oogenesis, Histology, Histochemistry

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