C Vitamininin Karbonik Anhidraz İzoenzimleri (hCA I ve II) Üzerine Etkisi

Year 2021, Volume: 21 Issue: 5, 1038 - 1045, 31.10.2021

Yağmur Hoşgör Ekrem Tunca , Metin Bülbül

https://doi.org/10.35414/akufemubid.890389

Abstract

Metabolik faaliyetlerin sorunsuz bir şekilde yerine getirilmesinde vitaminlerin önemi son derece büyüktür. Suda çözünen bir vitamin olan C vitamini, antioksidan özellik gösterir, nörotransmitasyonda ve kolajen sentezi gibi pek çok biyolojik süreçte rol alır. Karbonik anhidraz (CA) izoenzimleri ise elektrolit salınımı, pH dengesi, iyon taşınımı ve bunlarla ilişkili birçok süreçte rol alır. Çalışmamızda, C vitamininin, insan eritrositlerinden saflaştırılan hCA I ve hCA II üzerindeki inhibisyon etkileri in vitro olarak incelenmiştir. L-Askorbik asidin hCA I ve hCA II izoenzimlerini yarışmasız inhibisyon mekanizması üzerinden inhibe ettiği, Ki değerinin sırasıyla 96.8011.19 µM ve 120.4622.11 µM olduğu belirlenmiştir.

Keywords

C vitamini, Karbonik anhidraz, İnhibisyon, Glokom

Supporting Institution

Kütahya Dumlupınar Üniversitesi

Thanks

Çalışmalarımıza olan katkılarından dolayı Kütahya Dumlupınar Üniversitesi Bilimsel Araştırma Projeleri birimine teşekkür ederiz. Bu çalışma Yağmur HOŞGÖR'ün 2020 yılı öncesi tamamlanan yüksek lisans tezinden hazırlanmıştır.

The Effect of Vitamin C on Carbonic Anhydrase Isoenzymes (hCA I and II)

Abstract

Vitamins play an extremely important role in the smooth functioning of metabolic activities. Vitamin C, which is one of the water-soluble vitamins, has antioxidant properties and plays a role in many biological processes such as neurotransmission and collagen synthesis. Carbonic anhydrase (CA) enzymes play a role in electrolyte release, pH balance, ion transport and many related processes. In our study, the inhibition effects of vitamin C, on hCA I and hCA II purified from human erythrocytes were investigated as in vitro. It was determined that L-Ascorbic acid inhibits hCA I and hCA II isoenzymes through its non-competitive inhibition mechanism, and its Ki value was 96.8011.19 µM and 120.4622.11 µM, respectively.

Keywords

Vitamin C, Carbonic anhydrase, Inhibition, Glaucoma

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