Total Phenolic Content, Antioxidant Properties and Element Concentrations of Methanol and Acetone Extracts of Stachys tmolea Boiss.

Year 2022, Volume: 22 Issue: 6, 1271 - 1278, 28.12.2022

Laçine Aksoy Simge Demir

https://doi.org/10.35414/akufemubid.1099557

Abstract

DPPH radical scavenging, total antioxidant status, phenolic and element concentrations of the different solvent extracts of Stachys tmolea Boiss. were determined in the study. It was found that, the methanol extract of the plant contains total phenolic substance is 68.91±1.4 mg Gallic acid equivalant/g extract. % DPPH radical inhibition was 64.79±1.98 and total antioxidant status was 2.28±0.21 mmol Trolox Equiv./L. The acetone extract contains total phenolic substance (27.26±1.2 mg GAE/g extract). % DPPH radical was 50.33±2.05, and total antioxidant status was 0.57±0.04 mmol Trolox Equiv./L. It is seen that, the methanol extract has properties close to the synthetic antioxidant butylated hydroxy anisole (total phenolic substance concentration is 82.33±2.1 mg GAE/g). It is determined that the species also contains elements such as Cu, Mn, and Fe that participate in superoxide dismutase and catalase. In conclusion, the methanolic extract of Stachys tmolea exhibits radical scavenging and antioxidative properties. The species is thought to be a plant that can evaluate in phytotherapy studies due to the important elements it contains.

Keywords

Stachys tmolea, TAS, DPPH, Mineral

Stachys tmolea Boiss. in Metanol ve Aseton Ekstraktlarının Toplam Fenolik İçeriği, Antioksidan Özellikleri ve Element Konsantrasyonları

Abstract

Bu çalışmada Stachys tmolea Boiss. farklı çözücü ekstraktlarının DPPH radikal süpürücü ve toplam antioksidan aktivite ile fenolik ve element konsantrasyonları belirlenmiştir. Bitkinin metanol özütünün toplam fenolik madde içeriği 68.91±1.4 mg GAE/g ekstre olarak tespit edilmiştir. % DPPH radikal inhibisyonu 64.79±1.98 ve toplam antioksidan aktivitesi 2.28±0.21 mmol Trolox eşdeğeri/L dir. Aseton özütü 27,26±1,2 mg GAE/g ekstre toplam fenolik madde içerir. % DPPH radikali inhibisyonu 50.33±2.05 ve toplam antioksidan aktivitesi 0.57±0.04 mmol Trolox eşdeğeri/L dir. Metanol ekstraktının sentetik bir antioksidan olan bütillenmiş hidroksi anizole (toplam fenolik madde konsantrasyonu 82.33±2.1 mg GAE/g) yakın özelliklere sahip olduğu görülmektedir. Türün ayrıca süperoksit dismutaz ve katalaz enziminin yapısındfa bulunan Cu, Mn, Fe gibi elementleri de içerdiği belirlendi. Sonuç olarak, Stachys tmolea'nın metanolik özütü, radikal süpürücü ve antioksidatif özellikler sergiler. Türün içerdiği önemli elementler nedeniyle fitoterapi çalışmalarında değerlendirilebilecek bir bitki olduğu düşünülmektedir.

Keywords

Stachys tmolea, TAS, DPPH, Mineral

References

• Ahmad, N., Fazal, H., Ahmad, I., and Abbasi, B. 2011. Free Radical Scavenging (DPPH) Potential In Nine Mentha Species. Toxicology and Industrial Health, 28 (1), 83-89.
• Akcicek, E. 2010. A New Subspecies of Stachys Cretica (Section Eriostomum, Lamiaceae) from Turkey. Turkish Journal of Botany, 34, 131–136.
• Aksoy, L., and Senyer, G. Y. 2017. Bioelement Levels of Some Prominent Endemic Plant Species in Turkey. International Journal of Agriculture, Forestry and Fisheries, 5 (6), 107–112.
• Albayrak, S., Aksoy, A., Albayrak, S., and Sağdıç, O. 2013. In Vitro Antioxidant and Antimicrobial Activity of Some Lamiaceae Species. Iranian Journal of Science and Technology. Transaction A, Science, 1, 1–9.
• Amirghofran, Z., Bahmani, M., Azadmehr, A., and Javidnia, K. 2006. Anticancer Effects of Various Iranian Native Medicinal Plants on Human Tumor Cell Lines. Neoplasma, 53 (5), 428–433.
• Arai, S. 1996. Studies on Functional Foods in Japan: State of Art. Bioscience, Biotechnology, and Agrochemistry, 60, 9–15.
• Balasundram, N., Sundram, K., and Samman, S. 2006. Phenolic Compounds in Plants and Agri-Industrial by-Products: Antioxidant Activity, Occurrence, and Potential Uses. Food Chemistry, 99 (1), 191–203.
• Bhattacharjee, R. Stachys L. In Flora of Turkey and the East Agean Islands; Davis, P. H., Ed.; Edinburgh Univ. Press: Edinburgh, 1982; Vol. 7, pp 199–262.
• Bingöl, M.N. Determination of Antioxidant Actıvıty of Stachys lavandulifolia vahl. Var. Brachydon Boiss. by Different in vitro Methods. Msc. University of Muş Alparslan, Muş, 2016.
• Carović-Stanko, K., Petek, M., Grdiša, M., Pintar, J., Bedeković, D., Ćustić, M.H., and Satovic, Z. 2016. Medicinal Plants of the Family Lamiaceae as Functional Foods- A Review. Czech Journal of Food Sciences, 34 (5), 377–390.
• Duman, H., Kartal, M., Altun, L., Demirci, B., and Baser, K. H. C. 2005. The Essential Oils of Stachys Laetivirens Kotschy & Boiss. Ex Rech. Fil, Endemic in Turkey. Flavour and Fragrance Journal, 20, 48–50.
• Erel, O. 2004. A Novel Automated Method to Measure Total Antioxidant Response against Potent Free Radical Reactions. Clinical Biochemistry, 37 (2), 112–119.
• Gentili, R., Ambrosini, R., Montagnani, C., Caronni, S., and Citterio, S. 2018. Effect of Soil PH on the Growth, Reproductive Investment and Pollen Allergenicity of Ambrosia Artemisiifolia L. Frontiers in Plant Science 9, 1335.
• Grujic, S., Skaltsa, H. D., Marin, P., and Sokovic, M. 2004. Composition and Antimicrobial Activity of the Essential Oil of Six Stachys Species from Serbia. Flavour and Fragrance Journal, 19, 139–144.
• Güven, A., and Gürsul, I. 2014. Secondary Metabolite Synthesis In Plant Tissue Cultures. Gıda, 39 (5), 299-306.
• Kaya, A., Demirci, B., and Baser, K. H. C. 2001. The Composition of the Essential Oil of Stachys Iberica Subsp. Stenostachya Growing in Turkey. Chemistry of Natural Compounds, 37, 326–328.
• Khanavi, M., Sharifzadeh, M., Hadjiakhoondi, A., and Shafiee, A. 2005. Phytochemical Investigation and Anti-Inflammatory Activity of Aerial Parts of Stachys Byzanthina C. Koch. Journal of Ethnopharmacology, 97 (3), 463–468.
• Lagouri, V., and Alexandri, G. 2013. Antioxidant Properties of Greek O. Dictamnus and R. Officinalis Methanol and Aqueous Extracts-HPLC Determination of Phenolic Acids. International Journal of Food Properties, 16, 549–562.
• Leblebici, S. Doktora Tezi, An Investigation of the Anatomical and Ecological on Endemic Species of the Stachys sp. Spreading in Kütahya and Eskişehir. Dumlupınar University, Kütahya, 2011.
• Ličina, B. Z., Stefanović, O. D., Vasić, S. M., Radojević, I. D., Dekić, M. S., and Čomić, L. R. 2013. Biological Activities of the Extracts from Wild Growing Origanum Vulgare L. Food Control, 33 (2), 498–504.
• Mazid, M., Khan, T. A., and Mohammad, F. 2011. Role of Secondary Metabolites in Defense Mechanisms of Plants. Biology and Medicine, 3, 232–249.
• Ozkan, M. 2008. Glandular and Eglandular Hairs of Salvia Recognita Fisch. & Mey. (Lamiaceae) in Turkey. Bangladesh Journal of Botany, 37, 93–95.
• Oztürk, M., Duru, M. E., Aydoğmuş-Oztürk, F., Harmandar, M., Mahliçli, M., Kolak, U., and Ulubelen, A. 2009. GC-MS Analysis and Antimicrobial Activity of Essential Oil of Stachys Cretica Subsp. Smyrnaea. Natural Product Communications, 4 (1), 109–114.
• Perry, E., and Howes, M-JR. 2011. Medicinal plants and dementia therapy: Herbal hopes for brain aging? CNS Neuroscience & Therapeutics, 17, 683- 698.
• Skaltsa, H. D., Mavrommati, A., and Constantinidis, T. 2001. A Chemotaxonomic Investigation of Volatile Constituents in Stachys Subsect, Swainsonianeae (Labiatae). Phytochemistry, 57, 235–244.
• Sodré, A. C. B., Luz, J. M. Q., Haber, L. L., Marques, M. O. M., Rodrigues, C. R., and Blank, A. F. 2012. Organic and Mineral Fertilization and Chemical Composition of Lemon Balm (Melissa Officinalis) Essential Oil. Revista Brasileira de Farmacognosia, 22 (1), 40–44.
• Stamatis, G., Kyriazopoulos, P., Golegou, S., Basayiannis, A., Skaltsas, S., and Skaltsa, H. 2003. In Vitro Anti-Helicobacter Pylori Activity of Grek Herbal Medicines. Journal of Ethnopharmacology, 88, 175–179. Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L., and Hawkins Byrne, D. 2006. Comparison of ABTS, DPPH, FRAP, and ORAC Assays for Estimating Antioxidant Activity from Guava Fruit Extracts. Journal of Food Composition and Analysis, 19 (6–7), 669–675.
• Topcu, G., and Kusman, T. 2014. Lamiaceae Family Plants as a Potential Anticholinesterase Source in the Treatment of Alzheimer’s Disease. Bezmialem Science, 2 (1), 1–25.
• Trakoontivakorn, G., Tangkanakul, P., and Nakahara, K. 2012. Changes of Antioxidant Capacity and Phenolics in Ocimum Herbs after Various Cooking Methods. The Japan Agricultural Research Quarterly, 46 (4), 347–353.
• Vaishali Rai, M., Pai, R., Pratapchandra Kedilaya, V., and Hegde, H. 2013. Preliminary Phytochemical Screening of Members of Lamiaceae Family: Leucas Linifolia, Coleus Aromaticus and Pogestemon Patchouli. International Journal of Pharmaceutical Sciences Review and Research, 21, 131–137.
• Wink, M. 2003. Evolution of Secondary Metabolites from an Ecological and Molecular Phylogenetic Perspective. Phytochemistry, 64 (1), 3–19.
• Wojdylo, A., Oszmianski, J., and Czemerys, R. 2007. Antioxidant Activity and Phenolic Compounds in 32 Selected Herbs. Food Chemistry, 105 (3), 940–949.