Işık Yayan Diyot Dizisi ile Işık Mikroskobunda Faz-Kontrast Görüntüleme

Year 2023, Volume: 23 Issue: 2, 523 - 531, 03.05.2023

Nur Efşan Köksal İlyas Çankaya Esra Şengün Ermeydan

Abstract

Bu çalışmada, aydınlık-alan, karanlık-alan ve diferansiyel faz-kontrast görüntülerini aynı anda elde
edebilen tek-kameralı bir görüntüleme sistemi, programlanabilir bir ışık yayan diyot (LED) dizisi
aracılığıyla farklı aydınlatma modelleri ile birlikte kullanılmıştır. Kullanılan LED dizisi, aydınlatma
açılarının esnek bir şekilde modellenmesine olanak sağlamıştır. Sol-sağ, üst-alt ve eğik aydınlatmaların
etkisi, hem yanak epitel hücresinde hem de soğan zarı hücresinde detaylı olarak gözlemlenmiştir.
Aydınlık-alan, karanlık-alan ve diferansiyel faz-kontrast görüntüleri, 32x32 LED dizisinin desenleri
değiştirilerek herhangi bir hareketli parça olmadan elde edilmiştir. Bu nedenle, kullanılan sistem basittir,
düşük maliyetlidir ve geleneksel ışık mikroskobu ile uyumludur.

Keywords

Işık mikroskobu, LED dizisi, Faz-kontrast görüntüleme, 32x32 LED Matris, eğik aydınlatma

Supporting Institution

Ankara Yıldırım Beyazıt Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinatörlüğü

Project Number

AYBU-2018-BAP-4981

Thanks

Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Tıbbi Patoloji Anabilim Dalı öğretim üyesi Prof. Dr. Fazlı ERDOĞAN hocamıza mikroskop temininde yapmış oldukları katkılardan ve Ankara Yıldırım Beyazıt Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinatörlüğü’ne, AYBU-2018-BAP-4981 numaralı projeye vermiş olduğu maddi destekten dolayı teşekkür ederiz.

Phase-Contrast Imaging at Optical Microscope with a Light Emitting Diode Array

Abstract

In this study, a single-camera imaging system that can simultaneously acquire bright-field, dark-field
and differential phase-contrast images is used with different illumination patterns through a
programmable light-emitting diode (LED) array. The LED array allows flexible modeling of illumination
angles. The effect of left-right, top-bottom and oblique illumination was observed in detail both in the
cheek epithelial cell and in the onion membrane cell. Bright-field, dark-field and differential phasecontrast images are obtained without any moving parts by changing the patterns of 32x32 LED array.
Therefore, the system used is simple, low-cost and compatible with the conventional light microscopy.

Keywords

Optical microscope, LED array, Phase-contrast imaging, 32x32 LED matrix, oblique illumination

Project Number

AYBU-2018-BAP-4981

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