Year 2014,
Volume: 27 Issue: 2, 24 - 29, 31.12.2014
Abstract
In the study, the immunostimulant effects of the methanolic extract of nettle (Urtica dioica) on non-specific immune response of gold fish (Carassius auratus) were investigated. Two different concentration of extracts (0.1, 0.5 g/kg of feed) were individually mixed with the basal diet and fed to gold fish for 30 days. At the end of the study, blood samples were collected from caudal vein of the fish to assess the immunomodulatory characteristics of nettle using superoxide anion production (NBT), lysozyme, myeloperoxidase and phagocytic activity. In the study all immune parameters were affected positively by dietary nettle extract intake compared to control (P<0.05). The highest level of the nonspecific immune responses were determined in the 0.5% nettle group (P<0.05). According to study results, it can be say that nettle extract is an effective non-specific immunostimulant for gold fish
References
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- Bilen, S., Bulut, M., Bilen, A.M., 2011. Immunostimulant effects of Cotinus coggyria on rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 30: 451-455.
- Bilen, S., Yılmaz, S., Bilen, A.M., 2013. Influence of Tetra (Cotinus coggygria) Extract against Vibrio anguillarum Infection in Koi Carp, Cyprinus carpio with Reference to Haematological and Immunological Changes. Turkish Journal of Fisheries and Aquatic Sciences 13: 517-522.
- Bilen, S., Yılmaz, S., Bilen, A.M., Biswas, G., 2014. Effects of Dietary Incorporation of Tetra (Cotinus coggygria) Extract on Immune Response and Resistance to Aero- monas hydrophila in Koi Carp (Cyprinus carpio)., The Israeli Journal of Aqaucul- ture- Bamidgeh. 66:1-6.
- Binaii, M., Ghiasi, M., Farabi, S.M.V., Pougholam, R., Safari, R., Alavi, S.E., Taghavi, M.J., Bankehsaz, Z., 2014. Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica).Fish & Shellfish Immunology, 36: 46-51.
- Dügenci KS, Arda N, Candan A. Some medicinal plants as immunostimulant for fish. Jour- nal of Ethnopharmacology 2003;88:99e106. doi:10.1016/S0378-8741(03)00182-x.
- Hampton, M.B., Kettle, A.J. and Winterbourn, C.C. 1996. Involvement of superoxide and myeloperoxidase in oxygen-dependent killing of Staphylococcus aureus by neut- rophils. Infect Immun 64: 3512–3517.
- Kumari J. and P. K. Sahoo, 2006. Dietary levamisole modulates the immune response and disease resistance of Asian catfish Clarias batrachus (Linnaeus). Aquac. Res., 37:500-509.
- Quade, M.J. and Roth, J.A. 1997. A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet Immunol Immunop, 58: 239-248. doi: 10.1016/S0165-2427(97)00048-2
- Robertsen, B., 1999. Modulation of the non-specific defence of fish by structurally conser- ved microbial polymers. Fish Shellfish Immunol. 9, 269–290.
- Sahoo, P.K., Mukherjee, S.C., 2001. Dietary intake of levamisole improves non-specific immunity and disease resistance of healthy and aflatoxin induced immunocomp- romised roho (Labeo rohita). J. Appl. Aquacult. 11, 15–25.
- Siwicki AK, Anderson DP., 1993. Nonspecific Defense Mechanisms Assay in Fish. II. Potential Killing Activity of Neutrophils and Macrophages, Lysozyme Activity in Serum and Organs and Total Immunoglobulin Level in Serum. In: Siwicki AK, Anderson DP, Waluga J, editors. Fish Disease Diagnosis and Prevention Methods. Poland: Olsztyn; 1993. pp. 105–112.
- Siwicki, A.K., Anderson, D.P., Rumsey, G.L. 1994. Dietary intake of immunostimulants by rainbow trout effects non-specific immunity and protection against furuncluosis. Vet. Immunol. Immunopathol., 41: 125-139.
- Xie J., Liu B., Zhou Q., Su Y., He Y., Pan L., Ge X. And P. Xu, 2008. Effects of anthraqui- none extract from rhubarb Rheum officinale Bail on the crowding stress response and growth of common carp Cyprinus carpio var. Jian. Aquaculture, 281:5-11.
- Yin G., Jeney G., Racz T., Xu P., Jun X. and Z. Jeney, 2006. Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non–specific immune response of tila- pia, Oreochromis niloticus. Aquaculture, 253:39-47.
- Yin G., Ardo L., Jeney Z., Xu P. and G. Jeney, 2008. Chinese Herbs (Lonicera japonica and Ganoderma lucidum) enhance non-specific immune response of Tilapia, Ore- ochromis niloticus, and protection against Aeromonas hydrophila. Dis. Asian Aquac. IV, 269-281.
Isırgan otunun (Urtica dioica) metanolik özütünün japon balıklarının (Carassius auratus) doğal olmayan bağışıklık yanıtı üzerine etkileri
Abstract
Bu çalışmada ısırgan otunun metanolik özütünün japon balıklarının bağışıklık sistemi
üzerine etkileri incelenmiştir. Özütün iki farklı konsantrasyonu (0,1 ve 0,5 g/kg yem) japon
balığı yemi içerisine katılarak balıklar bu yemle 30 gün boyunca beslenmişlerdir. Çalışma
sonunda balıkların kaudal venasından kan örneği alınarak süperoksit anyon üretimi (NBT),
lizozim, myeloperoksidaz ve fagozitik aktivite gibi parametreler bağışıklık sisteminde
meydan gelen değişimleri belirlemek için kullanılmıştır. Çalışma sonunda tüm bağışıklık
yanıtların her iki ısırgan otu grubunda da kontrol grubu ile karşılaştırıldığında kayda değer
yüksek olduğu belirlenmiştir (P < 0.05). Isırgan otu grupları kendi içerisinde değerlendirildiğinde en yüksek bağışıklık yanıtlar % 0,5 ısırgan otu içeren grupta gözlenmiştir. Çalışma
sonuçlarına göre ısırgan otu özütünün japon balıkları için etkili bir bağışıklık uyarıcı olduğu
söylenebilir.
References
- Alexander, C.P., Kirubakaran, C.J.W., Michael, R.D. 2010. Water soluble fraction of Ti- nospora cordifolia leaves enhanced the non-specific immune mechanisms and di- sease resistance in Oreochromis mossambicus. Fish & Shellfish Immun., 29:765- 772.
- Bilen, S., Bulut, M., Bilen, A.M., 2011. Immunostimulant effects of Cotinus coggyria on rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 30: 451-455.
- Bilen, S., Yılmaz, S., Bilen, A.M., 2013. Influence of Tetra (Cotinus coggygria) Extract against Vibrio anguillarum Infection in Koi Carp, Cyprinus carpio with Reference to Haematological and Immunological Changes. Turkish Journal of Fisheries and Aquatic Sciences 13: 517-522.
- Bilen, S., Yılmaz, S., Bilen, A.M., Biswas, G., 2014. Effects of Dietary Incorporation of Tetra (Cotinus coggygria) Extract on Immune Response and Resistance to Aero- monas hydrophila in Koi Carp (Cyprinus carpio)., The Israeli Journal of Aqaucul- ture- Bamidgeh. 66:1-6.
- Binaii, M., Ghiasi, M., Farabi, S.M.V., Pougholam, R., Safari, R., Alavi, S.E., Taghavi, M.J., Bankehsaz, Z., 2014. Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica).Fish & Shellfish Immunology, 36: 46-51.
- Dügenci KS, Arda N, Candan A. Some medicinal plants as immunostimulant for fish. Jour- nal of Ethnopharmacology 2003;88:99e106. doi:10.1016/S0378-8741(03)00182-x.
- Hampton, M.B., Kettle, A.J. and Winterbourn, C.C. 1996. Involvement of superoxide and myeloperoxidase in oxygen-dependent killing of Staphylococcus aureus by neut- rophils. Infect Immun 64: 3512–3517.
- Kumari J. and P. K. Sahoo, 2006. Dietary levamisole modulates the immune response and disease resistance of Asian catfish Clarias batrachus (Linnaeus). Aquac. Res., 37:500-509.
- Quade, M.J. and Roth, J.A. 1997. A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet Immunol Immunop, 58: 239-248. doi: 10.1016/S0165-2427(97)00048-2
- Robertsen, B., 1999. Modulation of the non-specific defence of fish by structurally conser- ved microbial polymers. Fish Shellfish Immunol. 9, 269–290.
- Sahoo, P.K., Mukherjee, S.C., 2001. Dietary intake of levamisole improves non-specific immunity and disease resistance of healthy and aflatoxin induced immunocomp- romised roho (Labeo rohita). J. Appl. Aquacult. 11, 15–25.
- Siwicki AK, Anderson DP., 1993. Nonspecific Defense Mechanisms Assay in Fish. II. Potential Killing Activity of Neutrophils and Macrophages, Lysozyme Activity in Serum and Organs and Total Immunoglobulin Level in Serum. In: Siwicki AK, Anderson DP, Waluga J, editors. Fish Disease Diagnosis and Prevention Methods. Poland: Olsztyn; 1993. pp. 105–112.
- Siwicki, A.K., Anderson, D.P., Rumsey, G.L. 1994. Dietary intake of immunostimulants by rainbow trout effects non-specific immunity and protection against furuncluosis. Vet. Immunol. Immunopathol., 41: 125-139.
- Xie J., Liu B., Zhou Q., Su Y., He Y., Pan L., Ge X. And P. Xu, 2008. Effects of anthraqui- none extract from rhubarb Rheum officinale Bail on the crowding stress response and growth of common carp Cyprinus carpio var. Jian. Aquaculture, 281:5-11.
- Yin G., Jeney G., Racz T., Xu P., Jun X. and Z. Jeney, 2006. Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non–specific immune response of tila- pia, Oreochromis niloticus. Aquaculture, 253:39-47.
- Yin G., Ardo L., Jeney Z., Xu P. and G. Jeney, 2008. Chinese Herbs (Lonicera japonica and Ganoderma lucidum) enhance non-specific immune response of Tilapia, Ore- ochromis niloticus, and protection against Aeromonas hydrophila. Dis. Asian Aquac. IV, 269-281.
There are 16 citations in total.
Details
| Primary Language | tr:en |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 31, 2014 |
| Published in Issue | Year 2014 Volume: 27 Issue: 2 |