Reassessment of Mentha Species from Kunhar River Catchment Using Morphological and Molecular Markers

Year 2018, Volume: 28 Issue: 1, 6 - 12, 15.05.2018

İsrar Ahmad Sami Ullah Khan Aziz Khan Muhammad Shoaib Amjad Fozia Abbası

Abstract

ABSTRACT: Mentha specimens collected from
Kunhar River catchment of Hazara region were analyzed through numerical and
molecular markers. For numerical analysis twenty two traits were used.
Dendrogram analysis of morphological traits assorted the 25 Mentha collections into 4 groups viz,
Group-A, B, C and D. Group-A showed 98% similarity (M. longifolia). Group-B showed 98% similarity (M. spicata). Group-C showed 92%
similarity (M. arvensis) and Group-D
showed 89% similarity (M. royleana).
Molecular analyses were carried out through 11 RAPD primers. Primers
amplification revealed high level of genetic diversity (0-100%) existed among
the Mentha genotypes. Dendrogram
analyses based upon the genetic distance estimates conferred the cluster
analysis of the morphological traits. Furthermore, the variations among
populations of M. royleana need
further elaboration through additional marker assisted discrimination for
establishing their taxonomic status.

Keywords

Mentha, CTAB, DNA, PCR, RAPD, dendrogram, trait.

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