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A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus

Year 2013, Volume: 7 Issue: 1, 48 - 51, 01.10.2013
https://doi.org/10.2399/ana.11.212

Abstract

Objectives: The aim of the present study was to establish a simple and reliable method for separation of anteroventral periventricular (AVPV) and arcuate (Arc) nuclei in the rat hypothalamus to evaluate expression of peptide mRNAs in these nuclei using RT-PCR.

Methods: Two female Sprague -Dawley rats were perfused transcardially with 10% buffered formalin. Brains were immediately removed and diencephalon was dissected out by two coronal sections. The first section was made at rostral of optic chiasm and the second section at the caudal end of mammillary bodies. To separate the AVPV and Arc nuclei, the third section was made rostral of infundibulum which placed one part of the optic tract in rostral (anterior) division and its other part in the caudal (posterior) division. The rostral and caudal divisions were separately post-fixed and cut coronally and serially using a cryostat at 30-μm thickness. Sections from rostral and caudal divisions of the diencephalon were stained with cresyl violet. Landmarks for Arc and AVPV nuclei were evaluated in each section.

Results: Arc and AVPV nuclei were not simultaneously detected in any stained section in rostral or caudal divisions of diencephalon.

Conclusion: By the presented method, it is easily possible to separate AVPV and Arc nuclei to evaluate the expression pattern of mRNA of peptides expressed in these two hypothalamic nuclei of the rat by using RT-PCR or other molecular methods.  

References

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Year 2013, Volume: 7 Issue: 1, 48 - 51, 01.10.2013
https://doi.org/10.2399/ana.11.212

Abstract

References

  • Snell RS. Clinical Neuroanatomy. 7th ed. Philadelphia: Lippincott Williams & Wilkins; 2010.
  • Saper CB. Hypothalamus. In: Paxinos G, Mai JK, eds. The Human Nervous System. 2nd ed. Amsterdam: Elsevier; 2004. p. 513-50.
  • Dhillo WS. Kisspeptin: a novel regulator of reproductive function. J Neuroendocrinol 2008;20:963-70.
  • Simerly RB. Wired for reproduction: organization and develop- ment of sexually dimorphic circuits in the mammalian forebrain. Ann Rev Neurosci 2002;25:507-36.
  • Wiegand SJ, Terasawa E, Bridson WE, Goy RW. Effects of dis- crete lesions of preoptic and suprachiasmatic structures in the female rat. Neuroendocrinol 1980;31:147-57.
  • Ohkura S, Tsukamura H, Maeda K. Effects of various types of hypothalamic deafferentation on luteinizing hormone pulses in ovariectomized rats. J Neuroendocrinol 1991;3:503-8.
  • Clarkson J, Herbison AE. Postnatal development of kisspeptin neurons in mouse hypothalamus; sexual dimorphism and projec- tions to gonadotropin-releasing hormone neurons. Endocrinology 2006;147:5817-25.
  • Kauffman AS, Gottsch ML, Roa J, et al. Sexual differentiation of Kiss1 gene expression in the brain of the rat. Endocrinology 2007; 148:1774-83.
  • Smith JT, Clifton DK, Steiner RA. Regulation of the neuroen- docrine reproductive axis by kisspeptin-GPR54 signaling. Reproduction 2006;131:623-30.
  • Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. 6th ed. New York: Academic Press; 2007.
  • Quennell JH, Rizwan MZ, Relf HL, Anderson GM. Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland. J Neuroendocrinol 2010;22:309-16.
  • Knox AM, Li XF, Kinsey-Jones JS, et al. Neonatal lipopolysaccha- ride exposure delays puberty and alters hypothalamic Kiss1 and Kiss1r mRNA expression in the female rat. J Neuroendocrinol 2009; 21:683-9.
  • Kinsey-Jones JS, Li XF, Knox AM, et al. Down-Regulation of hypo- thalamic kisspeptin and its receptor, Kiss1r, mRNA expression is associated with stress-induced suppression of luteinising hormone secretion in the female rat. J Neuroendocrinol 2009;21:20-9.
  • Yamada S, Uenoyama Y, Kinoshita M, et al. Inhibition of metastin (kisspeptin-54)-GPR54 signaling in the arcuate nucleus-median eminence region during lactation in rats. Endocrinol 2007;148: 2226-32.
  • Kim SA, Jeon JH, Son MJ, Cha J, Chun MH, Kim IB. Changes in transcript and protein levels of calbindin D28k, calretinin and par- valbumin, and numbers of neuronal populations expressing these proteins in an ischemia model of rat retina. Anat Cell Biol 2010; 43:218-29.
There are 15 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Mohammad Saied Salehi This is me

Mohammad Reza Namavar This is me

Mohammad Reza Jafarzadeh Shirazi This is me

Farhad Rahmanifar This is me

Amin Tamadon This is me

Publication Date October 1, 2013
Published in Issue Year 2013 Volume: 7 Issue: 1

Cite

APA Salehi, M. S., Namavar, M. R., Shirazi, M. R. J., Rahmanifar, F., et al. (2013). A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus. Anatomy, 7(1), 48-51. https://doi.org/10.2399/ana.11.212
AMA Salehi MS, Namavar MR, Shirazi MRJ, Rahmanifar F, Tamadon A. A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus. Anatomy. October 2013;7(1):48-51. doi:10.2399/ana.11.212
Chicago Salehi, Mohammad Saied, Mohammad Reza Namavar, Mohammad Reza Jafarzadeh Shirazi, Farhad Rahmanifar, and Amin Tamadon. “A Simple Method for Isolation of the Anteroventral Periventricular and Arcuate Nuclei of the Rat Hypothalamus”. Anatomy 7, no. 1 (October 2013): 48-51. https://doi.org/10.2399/ana.11.212.
EndNote Salehi MS, Namavar MR, Shirazi MRJ, Rahmanifar F, Tamadon A (October 1, 2013) A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus. Anatomy 7 1 48–51.
IEEE M. S. Salehi, M. R. Namavar, M. R. J. Shirazi, F. Rahmanifar, and A. Tamadon, “A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus”, Anatomy, vol. 7, no. 1, pp. 48–51, 2013, doi: 10.2399/ana.11.212.
ISNAD Salehi, Mohammad Saied et al. “A Simple Method for Isolation of the Anteroventral Periventricular and Arcuate Nuclei of the Rat Hypothalamus”. Anatomy 7/1 (October 2013), 48-51. https://doi.org/10.2399/ana.11.212.
JAMA Salehi MS, Namavar MR, Shirazi MRJ, Rahmanifar F, Tamadon A. A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus. Anatomy. 2013;7:48–51.
MLA Salehi, Mohammad Saied et al. “A Simple Method for Isolation of the Anteroventral Periventricular and Arcuate Nuclei of the Rat Hypothalamus”. Anatomy, vol. 7, no. 1, 2013, pp. 48-51, doi:10.2399/ana.11.212.
Vancouver Salehi MS, Namavar MR, Shirazi MRJ, Rahmanifar F, Tamadon A. A simple method for isolation of the anteroventral periventricular and arcuate nuclei of the rat hypothalamus. Anatomy. 2013;7(1):48-51.

Anatomy is the official journal of Turkish Society of Anatomy and Clinical Anatomy (TSACA).