Investigation of Anticancer Activity of Ochratoxin Against Prostate Cancer Cell Line

Year 2025, Volume: 4 Issue: 3, 142 - 149

Neşe Başak Türkmen , İrem Sena Küçük Irmak Icen Taskın , Şeyma Yaşar , Dilan Aşkın Özek , Hande Yüce

Abstract

Prostate cancer is one of the most prevalent malignancies among men and remains a significant public health concern due to its high mortality rate, despite the availability of current treatment strategies. Ochratoxin, a naturally occurring mycotoxin, has attracted considerable attention for its diverse biological activities. This study explores the anticancer potential of ochratoxin in prostate cancer cell lines. Cell viability was evaluated following treatment with defined concentrations of ochratoxin, and apoptotic mechanisms, along with gene expression profiles, were analyzed. The results demonstrate that ochratoxin suppresses cell proliferation and induces apoptosis in a dose-dependent manner. These findings suggest that ochratoxin may represent a promising candidate for prostate cancer therapy. However, further in vivo studies are necessary to confirm its clinical relevance and therapeutic applicability.

Keywords

Prostate cancer , Ochratoxin , Anticancer activity , Apoptosis , Cell proliferation

Project Number

2209 projesi

References

• [1] IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. Lyon, France: World Health Organization; International Agency for Research on Cancer; 1993. Volume 56.
• [2] Więckowska M, Cichon N, Szelenberger R, Gorniak L, Bijak M. Ochratoxin A and Its Role in Cancer Development: A Comprehensive Review. Cancers. 2024;16(20):3473.
• [3]Meki AR, Hussein AA. Melatonin reduces oxidative stress induced by ochratoxin A in rat liver and kidney. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2001;130:305–313.
• [4] Liang R, Shen XL, Zhang B, Li Y, Xu W, Zhao C, Luo Y, Huang K. Apoptosis signal-regulating kinase 1 promotes Ochratoxin A-induced renal cytotoxicity. Scientific Reports. 2015;5:8078.
• [5] Ali, R., Khan, M. R., & Rehman, S. (2016). Toxicity study of ochratoxin A using HEK293 and HepG2 cell lines. Human & Experimental Toxicology, 35(5), 491–500.
• [6] Kumar, A., Sharma, V., & Singh, N. (2021). Studies on cytotoxic effects of ochratoxin A extracts on human cell lines (Hfl-1 and Hep-2). International Journal of Toxicological Research, 11(2), 45–52.
• [7] Skrzydlewski, P., Twarużek, M., & Grajewski, J. (2022). Cytotoxicity of mycotoxins and their combinations on different cell lines: A review. Toxins, 14(4), 244.
• [8] Heussner, A. H., & Bingle, L. E. (2015). Comparative ochratoxin toxicity: A review of the available data. Toxins, 7(10), 4253–4282.
• [9] Sava, V., Velasquez, A., Song, S., & Sanchez-Ramos, J. (2006). Effects of ochratoxin A on mitochondrial respiration and cell cycle in Vero cells. Toxicology and Applied Pharmacology, 213(2), 190–197.
• [10] Zhang, Y., Wang, Y., Li, X., Liu, Y., & Zhao, Y. (2018). Ochratoxin A induces apoptosis in MCF-7 cells via reactive oxygen species-mediated mitochondrial pathway. Food and Chemical Toxicology, 112, 1–10. [11] Migliaccio G, Ferraro R, Wang Z, Cristini V, Dogra P, Caserta S. Exploring Cell Migration Mechanisms in Cancer: From Wound Healing