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Application of Bacteriocin-Like Inhibitory Substances (BLIS)-Producing Probiotic Strain of Lactobacillus plantarum in Control of Staphylococcus aureus in White-Brined Cheese Production

Year 2019, Volume: 25 Issue: 4, 401 - 408, 05.12.2019
https://doi.org/10.15832/ankutbd.423833

Abstract

The aim of this study was to investigate the antimicrobial activity of an autochthonous probiotic strain of bacteriocin-like inhibitory substances (BLIS)-producing Lactobacillus plantarum, previously isolated from a Tulum cheese and satisfied technological criteria as adjunct culture in cheese production, in reducing Staphylococcus aureus during production and ripening of white-brined cheeses. Cheeses were manufactured in two trials from pasteurized milk artificially contaminated with S. aureus to the mean level of 6.243 log MPN mL-1. Lb. plantarum BG33 was added at 1% as adjunct to the starter culture. The study was also carried out with control group cheeses produced without the adjunct culture. S. aureus counts were monitored for up to 90 days by BAM’s 5-tube MPN method and each positive tube of MPN (most probable number) method was confirmed by PCR amplification of a 400 bp fragment of the nuc gene, which encodes the thermostable nuclease of S. aureus. The capacity of Lb. plantarum BG33 to reduce S. aureus count was found as 0.9 log unit on the 18th day of ripening. After 39 and 59 days of ripening, Lb. plantarum BG33 lowered S. aureus count by 1.9 and 2.0 log units, respectively, when compared to control group cheeses in which it was lowered by 0.5 and 1.0 log units, respectively. As a result, the BLIS activity of Lb. plantarum BG33 throughout ripening of white-brined cheese could make it useful as bioprotective adjunct culture in white-brined cheese production to prevent S. aureus growth which is an important foodborne pathogen in respect of safe cheese production.inhibitory substances (BLIS)-producing Lactobacillus plantarum, previously isolated from a Tulum cheese and satisfied technological criteria as adjunct culture in cheese production, in reducing Staphylococcus aureus during production and ripening of white-brined cheeses. Cheeses were manufactured in two trials from pasteurized milk artificially contaminated with S. aureus to the mean level of 6.243 log MPN mL-1. Lb. plantarum BG33 was added at 1% as adjunct to the starter culture. The study was also carried out with control group cheeses produced without the adjunct culture. S. aureus counts were monitored for up to 90 days by BAM’s 5-tube MPN method and each positive tube of MPN (most probable number) method was confirmed by PCR amplification of a 400 bp fragment of the nuc gene, which encodes the thermostable nuclease of S. aureus. The capacity of Lb. plantarum BG33 to reduce S. aureus count was found as 0.9 log unit on the 18th day of ripening. After 39 and 59 days of ripening, Lb. plantarum BG33 lowered S. aureus count by 1.9 and 2.0 log units, respectively, when compared to control group cheeses in which it was lowered by 0.5 and 1.0 log units, respectively. As a result, the BLIS activity of Lb. plantarum BG33 throughout ripening of white-brined cheese could make it useful as bioprotective adjunct culture in white-brined cheese production to prevent S. aureus growth which is an important foodborne pathogen in respect of safe cheese production.

References

  • Reference 1: Prof. Eugenia Bezirtzoglou Democritus University of Greece E-mail: empezirt@yahoo.gr
  • Reference 2: Assoc. Prof. İlkin Yücel Şengün Ege University E-mail: ilkin.sengun@ege.edu.tr
  • Reference 3: Assoc. Prof. Ali Topcu Hacettepe University E-mail: gali@hacettepe.edu.tr
Year 2019, Volume: 25 Issue: 4, 401 - 408, 05.12.2019
https://doi.org/10.15832/ankutbd.423833

Abstract

References

  • Reference 1: Prof. Eugenia Bezirtzoglou Democritus University of Greece E-mail: empezirt@yahoo.gr
  • Reference 2: Assoc. Prof. İlkin Yücel Şengün Ege University E-mail: ilkin.sengun@ege.edu.tr
  • Reference 3: Assoc. Prof. Ali Topcu Hacettepe University E-mail: gali@hacettepe.edu.tr
There are 3 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Birce Taban

Meltem Colaklar This is me

Sait Aykut Aytac

Hamdi Barbaros Özer This is me

Ayse Gursoy

Nefise Akcelik This is me

Publication Date December 5, 2019
Submission Date May 15, 2018
Acceptance Date September 6, 2018
Published in Issue Year 2019 Volume: 25 Issue: 4

Cite

APA Taban, B., Colaklar, M., Aytac, S. A., Özer, H. B., et al. (2019). Application of Bacteriocin-Like Inhibitory Substances (BLIS)-Producing Probiotic Strain of Lactobacillus plantarum in Control of Staphylococcus aureus in White-Brined Cheese Production. Journal of Agricultural Sciences, 25(4), 401-408. https://doi.org/10.15832/ankutbd.423833

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