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Genetic Diversity and Phylogenetic Relatıonshıps of Turkish Local Popcorn (Zea mays everta) Populations By Simple Sequence Repeats (SSRs)

Year 2021, Volume: 27 Issue: 2, 170 - 178, 04.06.2021
https://doi.org/10.15832/ankutbd.626912

Abstract

Maize (Zea mays everta) is preferred as a good dietary in Turkey and it is important to know its genetic diversity to improve the yield. Genetic markers are very important in determining genetic diversity in popcorn populations. The aim of this study was to evaluate the genetic diversity of landraces popcorn populations by simple sequence repeats (SSR) markers. A hundred seventy five accessions of popcorn from thirty five populations grown in Turkey were analyzed using twenty SSR markers. As a result of molecular analysis, 65 of 66 alleles obtained were showed polymorphisms and the polymorphism rate was 98.5%. The average number of alleles for each SSR loci was 3.3, and this the number of alleles varied from 1 to 5. The average the polymorphism information content (PIC) value was calculated to be 0.57 for SSR locus ranging from 0.00 to 0.89. The number and percentage of polymorphic loci of the genotypes were determined to vary between 29/47% and 43.94/71.21 % and the mean values were calculated as 39.114 and 59.265 % respectively. The value of genetic change in the phylogenetic tree obtained from landraces popcorn populations was determined as 0.05, and the genetic difference among genotypes varied from 14.7 to 97.1%. Among the markers used in the study, it was observed that code ‘phi064’ was the most effective marker for determining genetic diversity in popcorn and the highest allele frequency also on this marker was obtained.

Supporting Institution

Scientific Research Projects Unit of the Kahramanmaras Sutcu Imam University

Project Number

2014/3-27 D

Thanks

This study was supported by the Scientific Research Projects Unit of the Kahramanmaras Sutcu Imam University (Project number: 2014/3-27 D).

References

  • Aci M M, Revilla P, Morsli A, Djemel A, Belalia N, Kadri Y, Khelifi-Saloui M, Ordás B & Khelifi L (2013). Genetic diversity in Algerian maize (Zea mays L.) landraces using SSR markers. Maydica, 58., 304-310.
  • Adjanohoun A, Allagbe M, Noumavo P A, Gotoechan-Hodonou H, Sikirou R, Dossa K K, GleleKakaï R, Kotchoni S O & Baba-Moussa L (2011). Effects of plant growth promoting rhizobacteria on field grown maize. Journal of Animal & Plant Sciences, 11(3), 1457-1465.
  • Atanda A S & Olaoye G (2017). Multiplex-Ready PCR assay of SSR marker diversity among quality protein maize inbred parental lines. South African Journal of Plant and Soil, 34 (2), 149-154.
  • Comertpay G (2008). Yerel mısır populasyonlarının morfolojik ve DNA molekuler isaretleyicilerinden SSR teknigi ile karakterizasyonu. Cukurova Universitesi, Fen Bilimleri Enstitusu, Tarla Bitkileri Ana Bilim Dalı, Doktora Tezi, Adana.
  • Doyle J J & Doyle J L (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, v.19, p.11-15.
  • Eschholz T W, Stamp P, Peter R, Leipner J & Hund A (2010). Genetic structure and history of swiss maize (Zea mays L. ssp. mays) landraces. Genetic Resources and Crop Evolution, 57(1), 71-84.
  • Frankel O H (1972). The significance, utilization and conservation of crop genetic resources. FAO, Rome.
  • Gauthier P, Gouesnard B, Dallard J, Redaelli R, Rebourg C, Charcosset A, Boyat A (2002). RFLP diversity and relationships among traditional european maize populations. Theoretical and Applied Genetics, 105(1), 91-99.
  • Kimura M & Crow J F (1964). The Number of alleles that can be maintained in a finite population. Genetics, 49(4), 725.
  • Laborda P R, Oliveira K M, Garcia A A F, Paterniani M E A G Z, De Souza A P (2005). Tropical maize germplasm: What can we say about its genetic diversity in the light of molecular markers? Theoretical and Applied Genetics, 111(7), 1288-1299.
  • Lewontin R C (1972). The Apportionment of human diversity. Evolutionary Biology, 6(381), e398.
  • Liu W S, Dong M, Song Z P, Wei W (2009). Genetic diversity pattern of Stipapurpurea populations in the hinterland of Qinghai-Tibet Plateau. Annals of Applied Biology, 154: 57–65.
  • McDermott J M, McDonald B A (1993). Gene flow in plant pathosystems. Annual Review of Phytopathology, 31(1), 353-373.
  • Molin D, Coelho C J, Máximo D S, Ferreira F S, Gardingo J R, Matiello R R (2013). Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers. Genetics and Molecular Research, 12(1),99-114.
  • Nei M (1972). Genetic distance between populations. The American Naturalist, 106(949), 283.
  • Nei M (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, 70(12), 3321.
  • Nei M (1978). The theory of genetic distance and evolution of human races. Japanese Journal of Human Genetics, 23(4), 341.
  • Nei M (1987). Genetic distance and molecular phylogeny. Population genetics and fishery management, 193, p.223.
  • Ribeiro C A G, Pinto M D O, Maciel T E F, Pastina M M, Barros E G D, Guimarães C T (2017). Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids. Scientia Agricola, 74(2), 163-168.
  • Rohlf F J (1992). Program numerical taxonomy and multivariate analysis system. Version 1.70, New York.
  • Sharma L, Prasanna B M, Ramesh B (2010). Analysis of phenotypic and microsatellite-based diversity of maize landraces in India, especially from the North East Himalayan region. Genetica, 138(6), 619-631.
  • Tahir N A & Maeruf M S (2016). Assessment of salinity tolerance and SSR profile differentiation in nine maize genotypes (Zea mays L). Maydica, 61, M18.
  • Vivodík M, Gálová Z, Balážová Ž, Petrovičová L (2017). Genetic variation of european maize genotypes (Zea mays L.) detected using SSR markers. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 126-131.
  • Warburton M L, Ribaut J M, Franco J, Crossa J, Dubreuil P, Betrán FJ (2005). Genetic characterization of 218 elite CIMMYT inbred maize lines using RFLP markers. Euphytica, 142:97–106.
  • Yao Q L, Yang K C, Pan G T, Rong T Z (2008). Genetic diversity of maize (Zea mays L.) landraces from southwest China based on SSR data. J. Genet. Genomics, 34: 851-860.
  • Zhang J, Guo J, Liu Y, Zhang D, Zhao Y, Zhu L, Huang Y, Zhang Z, Chen J (2016). Genome-wide association study identifies genetic factors for grain filling rate and grain drying rate in maize. Euphytica, 212(2), 201-21.
Year 2021, Volume: 27 Issue: 2, 170 - 178, 04.06.2021
https://doi.org/10.15832/ankutbd.626912

Abstract

Project Number

2014/3-27 D

References

  • Aci M M, Revilla P, Morsli A, Djemel A, Belalia N, Kadri Y, Khelifi-Saloui M, Ordás B & Khelifi L (2013). Genetic diversity in Algerian maize (Zea mays L.) landraces using SSR markers. Maydica, 58., 304-310.
  • Adjanohoun A, Allagbe M, Noumavo P A, Gotoechan-Hodonou H, Sikirou R, Dossa K K, GleleKakaï R, Kotchoni S O & Baba-Moussa L (2011). Effects of plant growth promoting rhizobacteria on field grown maize. Journal of Animal & Plant Sciences, 11(3), 1457-1465.
  • Atanda A S & Olaoye G (2017). Multiplex-Ready PCR assay of SSR marker diversity among quality protein maize inbred parental lines. South African Journal of Plant and Soil, 34 (2), 149-154.
  • Comertpay G (2008). Yerel mısır populasyonlarının morfolojik ve DNA molekuler isaretleyicilerinden SSR teknigi ile karakterizasyonu. Cukurova Universitesi, Fen Bilimleri Enstitusu, Tarla Bitkileri Ana Bilim Dalı, Doktora Tezi, Adana.
  • Doyle J J & Doyle J L (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, v.19, p.11-15.
  • Eschholz T W, Stamp P, Peter R, Leipner J & Hund A (2010). Genetic structure and history of swiss maize (Zea mays L. ssp. mays) landraces. Genetic Resources and Crop Evolution, 57(1), 71-84.
  • Frankel O H (1972). The significance, utilization and conservation of crop genetic resources. FAO, Rome.
  • Gauthier P, Gouesnard B, Dallard J, Redaelli R, Rebourg C, Charcosset A, Boyat A (2002). RFLP diversity and relationships among traditional european maize populations. Theoretical and Applied Genetics, 105(1), 91-99.
  • Kimura M & Crow J F (1964). The Number of alleles that can be maintained in a finite population. Genetics, 49(4), 725.
  • Laborda P R, Oliveira K M, Garcia A A F, Paterniani M E A G Z, De Souza A P (2005). Tropical maize germplasm: What can we say about its genetic diversity in the light of molecular markers? Theoretical and Applied Genetics, 111(7), 1288-1299.
  • Lewontin R C (1972). The Apportionment of human diversity. Evolutionary Biology, 6(381), e398.
  • Liu W S, Dong M, Song Z P, Wei W (2009). Genetic diversity pattern of Stipapurpurea populations in the hinterland of Qinghai-Tibet Plateau. Annals of Applied Biology, 154: 57–65.
  • McDermott J M, McDonald B A (1993). Gene flow in plant pathosystems. Annual Review of Phytopathology, 31(1), 353-373.
  • Molin D, Coelho C J, Máximo D S, Ferreira F S, Gardingo J R, Matiello R R (2013). Genetic diversity in the germplasm of tropical maize landraces determined using molecular markers. Genetics and Molecular Research, 12(1),99-114.
  • Nei M (1972). Genetic distance between populations. The American Naturalist, 106(949), 283.
  • Nei M (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, 70(12), 3321.
  • Nei M (1978). The theory of genetic distance and evolution of human races. Japanese Journal of Human Genetics, 23(4), 341.
  • Nei M (1987). Genetic distance and molecular phylogeny. Population genetics and fishery management, 193, p.223.
  • Ribeiro C A G, Pinto M D O, Maciel T E F, Pastina M M, Barros E G D, Guimarães C T (2017). Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids. Scientia Agricola, 74(2), 163-168.
  • Rohlf F J (1992). Program numerical taxonomy and multivariate analysis system. Version 1.70, New York.
  • Sharma L, Prasanna B M, Ramesh B (2010). Analysis of phenotypic and microsatellite-based diversity of maize landraces in India, especially from the North East Himalayan region. Genetica, 138(6), 619-631.
  • Tahir N A & Maeruf M S (2016). Assessment of salinity tolerance and SSR profile differentiation in nine maize genotypes (Zea mays L). Maydica, 61, M18.
  • Vivodík M, Gálová Z, Balážová Ž, Petrovičová L (2017). Genetic variation of european maize genotypes (Zea mays L.) detected using SSR markers. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 126-131.
  • Warburton M L, Ribaut J M, Franco J, Crossa J, Dubreuil P, Betrán FJ (2005). Genetic characterization of 218 elite CIMMYT inbred maize lines using RFLP markers. Euphytica, 142:97–106.
  • Yao Q L, Yang K C, Pan G T, Rong T Z (2008). Genetic diversity of maize (Zea mays L.) landraces from southwest China based on SSR data. J. Genet. Genomics, 34: 851-860.
  • Zhang J, Guo J, Liu Y, Zhang D, Zhao Y, Zhu L, Huang Y, Zhang Z, Chen J (2016). Genome-wide association study identifies genetic factors for grain filling rate and grain drying rate in maize. Euphytica, 212(2), 201-21.
There are 26 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Gülay Zulkadir 0000-0003-3488-4011

Leyla İdikut 0000-0002-0685-7158

Project Number 2014/3-27 D
Publication Date June 4, 2021
Submission Date September 30, 2019
Acceptance Date December 26, 2019
Published in Issue Year 2021 Volume: 27 Issue: 2

Cite

APA Zulkadir, G., & İdikut, L. (2021). Genetic Diversity and Phylogenetic Relatıonshıps of Turkish Local Popcorn (Zea mays everta) Populations By Simple Sequence Repeats (SSRs). Journal of Agricultural Sciences, 27(2), 170-178. https://doi.org/10.15832/ankutbd.626912

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