Characterization and antimicrobial properties of silver nanoparticules biosynthesized from cornelian cherry (Cornus mas L.)

Year 2024, Volume: 30 Issue: 3, 444 - 457, 23.07.2024

Tuğçe Özeşer Nural Karagözlü

https://doi.org/10.15832/ankutbd.1332427

Abstract

Nanoparticles produced by green synthesis has been increasingly gaining popularity, especially because they are eco-friendly and low cost. In the present article, silver nanoparticles (AgNPs) were synthesized from the extracts prepared using cornelian cherry (Cornus mas L.) at two different temperatures. The properties of obtained AgNPs were determined through UV-Vis spectroscopy, SEM, EDX, FTIR, and XRD analyses, and their antimicrobial effects on four pathogenic bacteria were investigated. The analysis results conducted using UV-spectrophotometry, SEM, EDX, FTIR, and XRD on AgNPs prepared from extracts obtained at two different temperatures (20 °C and 60 °C) were similar. The groups playing a role in nanoparticle formation were determined to be C=C, C=O, and C-O, and it was also concluded that the two different extraction temperatures had no significant effect on nanoparticle synthesis and characterization. The cherry extract's antimicrobial activity was effective against Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhi, while it didn’t show activity against Escherichia coli O157:H7. The AgNPs at concentration of 25 mg/mL created inhibition zones of 9 mm, 9 mm, and 7 mm for L.monocytogenes, S.aureus, and S.Typhi, respectively, at 20 °C. It was seen that 25 mg/mL AgNPs synthesized at 60 °C formed 9 mm and 8 mm inhibition zones in S.aureus and L.monocytogenes cultures, respectively, whereas they showed no inhibiting activity against S.Typhi, and E.coli O157:H7. It has been seen that 20 °C has ease of application in two different temperatures applied in the preparation of silver nanoparticles and is a good alternative to chemical methods.

Keywords

silver nanoparticles, antimicrobial effects, cornelian cherry, Cornus mas L., green synthesis

Supporting Institution

Manisa Celal Bayar University Scientific Research Projects Coordination Unit.

Project Number

Project Number: 2021-033

References

• Abbasi E, Milani M, Fekri Aval S, Kouhi M, Akbarzadeh A, Tayefi Nasrabadi H, Nikasa P, Joo S.W Hanifehpour Y & Nejati-Koshki K (2016). "Silver nanoparticles: synthesis methods, bio-applications and properties". Critical reviews in microbiology 42: 173-180. https://doi.org/10.3109/1040841x.2014.912200
• Ali Z A, Yahya R, Sekaran S D & Puteh R (2016). “Green synthesis of silver nanoparticles using apple extract and its antibacterial properties”. Advances in Materials Science and Engineering pp. 1-6. https://doi.org/10.21275/sr231021141519
• Alkhattaf F S (2021). “Gold and silver nanoparticles: Green synthesis, microbes, mechanism, factors, plant disease management and environmental risks“ Saudi Journal of Biological Sciences 28(6): 3624-3631
• Alkhalaf M I, Hussein R H & Hamza A (2020). “Green synthesis of silver nanoparticles by Nigellasativa extract alleviates diabetic neuropathy through anti-inflammatory and antioxidanteffects”. Saudi Journal of Biological Sciences 27(9): 2410-2419. https://doi.org/10.1016/j.sjbs.2020.05.005
• Ameen F, Srinivasan P, Selvankumar T, Kamala-Kannan S, Al Nadhari S, Almansob A, Dawoud T & Govarthanan M (2019). “Phytosynthesis of silver nanoparticles using Mangifera indica flower extract as bioreductant and their broad-spectrum antibacterial activity”” Bioorganic Chemistry 88: 102970
• Arshadi E, Sedaghat S & Moradi O (2018). “Green synthesis and characterization of silver nanoparti cles using fructose”. Asian Journal of Green Chemistry 2(1): 41-50
• Arya A, Mishra V & Chundawat T S (2019). “Green synthesis of silver nanoparticles from green algae (Botryococcus braunii) and its catalytic behavior for the synthesis of benzi.midazoles”. Chemical Data Collections 20: 100190 Baran M F, Umaz A & Eren A (2020). “Antimicrobial applications of silver nanoparticles synthesized from plant origin against some microorganisms” Fen Bilimleri ve Matematik Alanında Araştırma ve Derlemeler 93-100. (In Turkish)
• Baysal G (2020). “The use of nanosystems in the food industry” The Journal of Food 45(3): 517-529. (In Turkish)
• Behravan M, Panahi A H, Naghizadeh A, Ziaee M, Mahdavi R & Mirzapour A (2019). “Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity” International journal of biological macromolecules 124: 148-154. https://doi.org/10.1016/j.ijbiomac.2018.11.101
• Cao Y, Jin R & Mirkin C A (2001). "DNA-Modified Core− Shell Ag/Au Nanoparticles". Journal of the American Chemical Society 123: 7961-7962
• Celep E, Aydın A & Yeşilada E (2012). “A comparative study on the in vitro antioxidant potentials of three edible fruits: Cornelian cherry, Japanese persimmon and cherry laurel“ Food and Chemical Toxicology 50(9): 3329-3335). https://doi.org/10.1016/j.fct.2012.06.010
• Ceylan R, Demirbas A, Ocsoy İ, & Aktumsek A (2021). “Green synthesis of silver nanoparticles using aqueous extracts of three Sideritis species from Turkey and evaluations bioactivity potentials”. Sustainable Chemistry and Pharmacy 21: 100426
• David L, Moldovan B, Baldea I, Olteanu D, Bolfa P, Clichici S & Filip G A (2020). “Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats”. Materials Science and Engineering: C, 110: 110709. https://doi.org/10.1016/j.msec.2020.110709
• Diler D & Leblebicier Y (2020). “Muşmula (Mespilus germanica L.) Silver nanoparticle (AgNP) synthesis and characterization studies in medlar (Mespilus germanica L.) extract biocatalyst”. Bilim Armonisi, 3(1): 17-23. https://doi.org/10.37215/bilar.595127 (In Turkish)
• Du J, Hu Z, Yu Z, Li H, Pan J, Zhao D & Bai Y (2019). “Antibacterial activity of a novel Forsythia suspensa fruit mediated green silver nanoparticles against food-borne pathogens and mechanisms investigation”. Materials Science and Engineering: C, 102: 247-253. https://doi.org/10.1016/j.msec.2019.04.031
• Ekrikaya S, Yilmaz E, Celik C, Demirbuga S, Ildiz N, Demirbas A & Ocsoy İ (2021). “Investigation of ellagic acid rich-berry extracts directed silver nanoparticles synthesis and their antimicrobial properties with potential mechanisms towards Enterococcus faecalis and Candida albicans”. Journal of Biotechnology 341: 155–162
• Filip G A, Moldovan B, Baldea I, Olteanu D, Suharoschi R, Decea N, Cismaru C M, Gal E, Cenariu M, Clichici S & David L (2019). “UV-light mediated green synthesis of silver and gold nanoparticles using cornelian cherry fruit extract and their comparative effects in experimental inflammation”. Journal of Photochemistry and Photobiology B: Biology 191: 26-37. https://doi.org/10.1016/j.jphotobiol.2018.12.006
• Ghaedi M, Yousefinejad M, Safarpoor M, Khafri H Z & Purkait M K (2015). “Rosmarinus officinalis leaf extract mediated green synthesis of silver nanoparticles and investigation of its antimicrobial properties”. Journal of Industrial and Engineering Chemistry 31: 167-172. https://doi.org/10.1016/j.jiec.2015.06.020
• Gulbagca F, Ozdemir S, Gulcan M & Sen F (2019). “Synthesis and characterization of Rosa canina-mediated biogenic silver nanoparticles for anti-oxidant, antibacterial, antifungal, and DNA cleavage activities”. Heliyon 5(12): e02980
• Hashim N, Paramasivam M, Tan J S, Kernain D, Hussin M H, Brosse N, Gambier F & Raja P B (2020). “Green mode synthesis of silver nanoparticles using Vitis vinifera’s tannin and screening its antimicrobial activity/apoptotic potential versus cancer cells”. Materials Today Communications 25: 101511. https://doi.org/10.1016/j.mtcomm.2020.1 01511
• He Y, Li X, Wang J, Yang Q, Yao B, Zhao Y, Zhao A, Sun W & Zhang Q (2017). “Synthesis, characterization and evaluation cytotoxic activity of silver nanoparticles synthesized by Chinese herbal Cornus officinalis via environment friendly approach”. Environmental Toxicology and Pharmacology 56: 56-60
• Hernandez-Morales L, Espinoza-Gómez H, Flores-López L Z, SoteloBarrera E L, Núñez-Rivera A, Cadena-Nava R D, Alonso- Núñez G & Espinoza K A (2019). “Study of the green synthesis of silver nanoparticles using a natural extract of dark or white Salvia hispanica L. seeds and their antibacterial application”. Applied Surface Science 489: 952-961. https://doi.org/10.1016/j.apsusc.2019.06.031
• Hernandez-Pinero J L, Terrón-Rebolledo M, Foroughbakhch R, Moreno-Limón S, Melendrez M F, Solís-Pomar F & Pérez-Tijerina E (2016). “Effect of heating rate and plant species on the size and uniformity of silver nanoparticles synthesized using aromatic plant extracts”. Applied Nanoscience 6(8): 1183-1190
• KaviyaS, Santhanalakshmi J, Viswanathan B, Muthumary J & Srinivasan K (2011). “Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity”. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 79(3): 594-598. https://doi.org/10.1016/j.saa.2011.03.040
• Korkmaz N, Ceylan Y, Hamid A, Karadağ A, Bülbül A S, Aftab M N, Çevik Ö & Şen F (2020). “Biogenic silver nanoparticles synthesized via Mimusops elengi fruit extract, a study on antibiofilm, antibacterial, and anticancer activities”. Journal of Drug Delivery Science and Technology 59: 101864. https://doi.org/10.1016/j.jddst.2020.101864
• Kumar B, Smita K, Cumbal L & Debut A (2017a). “Green synthesis of silver nanoparticles using Andean blackberry fruit extract”. Saudi journal of biological sciences 24(1): 45-50. https://doi.org/10.1016/j.sjbs.2015.09.006
• Kumar R, Ghoshal G, Jain A & Goyal M (2017b). “Rapid green synthesis of silver nanoparticles (AgNPs) using (Prunus persica) plants extract: exploring its antimicrobial and catalytic activities”. J. Nanomed. Nanotechnol, 8(4): 1-8
• Lopes C R B & Courrol L C (2018). “Green synthesisof silver nanoparticles with extract of Mimusopscoriacea and light”. Journal of Luminescence, 199: 183-187
• Paiva-Santos A C, Herdade A M, Guerra C, Peixoto D, Pereira-Silva M, Zeinali M, Mascarenhas-Melo F, Paranhos A & Veiga F (2021). “Plant-mediated green synthesis of metal-based nanoparticles for dermopharmaceutical and cosmetic applications”. International Journal of Pharmaceutics 597: 120311
• Pallela P N V K, Ummey S, Ruddaraju L K, Pammi S V N & Yoon S G (2018). “Ultra Small, monodispersed green synthesized silver nanoparticlesusing aqueous extract of Sida cordifolia plant andinvestigation of antibacterial activity”. Microbial Pathogenesis 124: 63-69. https://doi.org/10.1016/j.micpath.2018.08.026
• Pirtarighat S, Ghannadnia M & Baghshahi S (2019). “Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment”. Journal of Nanostructure in Chemistry 9(1): 1-9
• Polat S & Fenercioğlu H (2014). “Nanotechnology applications in food packaging: Use of inorganic nanoparticles”. Gıda 39(3): 187-194. (In Turkish)
• Rai V R & Bai J (2011). “Nanoparticles and their potential application as antimicrobials”. Ed.: A. Méndez-Vilas, Microbiology Series No. 3 Vol. 1, Spain pp. 197-209
• Ramkumar V S, Pugazhendhi A, Gopalakrishnan K, Sivagurunathan P, Saratale G D, Dung T & Kannapiran E (2017). “Biofabricationand characterization of silver nanoparticles usingaqueous extract of seaweed Enteromorphacompressa and its biomedical properties”. Biotechnology Reports 14: 1-7
• Rana A, Yadav K & Jagadevan S (2020). “A comprehensive review on green synthesis of nature-inspired metal nanoparticles: mechanism, application and toxicity”. Journal of Cleaner Production 272: 1-25. https://doi.org/10.1016/j.jclepro.2020.122880
• Rezvani E, Rafferty A, McGuinness C & Kennedy J (2019). “Adverse effects of nanosilver on human health and the environment”. Acta Biomaterialia 94: 145-159
• Salejda A M, Kucharska A Z & Krasnowska G (2018). “Effect of cornelian cherry (Cornus mas L.) juice on selected quality properties of beef burgers”. Journal of Food Quality, Article ID 1563651. https://doi.org/10.1155/2018/156365
• Saravanakumar A, Peng M M, Ganesh M, Jayaprakash J, Mohankumar M & Jang H T (2017). “Low-cost and eco-friendly green synthesis of silver nanoparticles using Prunus japonica (Rosaceae) leaf extract and their antibacterial, antioxidant properties”. Artificial Cells, Nanomedicine, and Biotechnology 45(6): 1165-1171
• Silver S (2003). “Bacterial silver resistance: Moleculer biology and uses and misuses of silver compounds”. FEMS Microbiology Reviews 27(2-3): 341-353
• Stankovic M S, Zia-Ul-Haq M, Bojovic B M & Topuzovic M D (2014). “Total phenolics, flavonoid content and antioxidant power of leaf, flower and fruits from cornelian cherry (Cornus mas L.)”. Bulgarian Journal of Agricultural Science 20(2): 358-363.
• Sürengil G & Kılınç B (2011). “Gıda ambalaj sektöründe nanoteknolojik uygulamalar ve su ürünleri açısından önemi”. Journal of Fisheries Sciences 5(4): 317-325
• Taghavizadeh Yazdi M E, Modarres M, Amiri M S & Darroudi M (2019). “Phyto-synthesis of silver nanoparticles using aerial extract of Salvia leriifolia Benth and evaluation of their antibacterial and photo-catalytic properties”. Research on Chemical Intermediates 45(3): 1105-1116. https://doi.org/10.1007/s11164-018-3666-8
• Tailor G, Yadav B L, Chaudhary J, Joshi M & Suvalka C (2020). “Green synthesis of silver nanoparticles using Ocimum canum and their anti-bacterial activity”. Biochemistry and Biophysics Reports 24: 100848. https://doi.org/10.1016/j.bbrep.2020.100848
• Thomas B, Vithiya B, Prasad T, Mohamed S B, Magdalane C M, Kaviyarasu K & Maaza M (2019). “Antioxidant and photocatalytic activity of aqueous leaf extract mediated green synthesis of silver nanoparticles using Passiflora edulis f. flavicarpa”. Journal of Nanoscience and Nanotechnology 19(5): 2640-2648
• Vignesh, V, Anbarasi K F, Karthikeyeni S, Sathiyanarayanan G, Subramanian P & Thirumurugan R (2013). “A superficial phyto-assisted synthesis of silver nanoparticles and their assessment on hematological and biochemical parameters in Labeo rohita (Hamilton, 1822)”. Colloids and Surfaces A: Physicochemical and Engineering Aspects 439: 184-192. https://doi.org/10.1016/j.colsurfa.2013.04.011
• Vijayakumar S, Malaikozhundan B, Saravanakumar K, Durán-Lara E F, Wang M H & Vaseeharan B (2019). “Garlic clove extract assisted silver nanoparticle–Antibacterial, antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity assessment”. Journal of Photochemistry and Photobiology B: Biology 198: 111558
• Wang S, Lawson R, Ray P C & Yu H (2011). “Toxic effects of gold nanoparticles on Salmonella Typhimurium bacteria”. Toxicology and Industrial Health 27(6): 547-554