Characterization of the Volatile Profile of Bee Venom from Different Regions in Türkiye Using Gas Chromatography-Mass Spectrometry
Year 2025,
Volume: 31 Issue: 1, 22 - 32, 14.01.2025
Buket Aydeniz-güneşer
,
Onur Güneşer
,
Meral Kekeçoğlu
,
Sevgi Kolaylı
Abstract
The volatile organic compounds of bee venoms from four different populations of Apis mellifera anatoliaca, came from different regions in Türkiye, were analyzed using solid phase microextraction technique combined with gas chromatography-mass spectrometry. A total of 144 volatile compounds were identified in the bee venom samples. The identified volatile compounds included esters, terpenoids, alcohols, acid esters, aldehydes, ketones, and hydrocarbons. It was determined that ester-type volatile compounds characterized the bee venom obtained from the Central Anatolia Region, while bee venom from the Western Black Sea Region had a higher amount of volatile terpenes with spicy and woody aromas. Further studies are required to understand the volatile profile of bee venom, which consists of plant and animal secondary metabolites.
Ethical Statement
This research does not require ethical approval.
Thanks
The authors are greatly indebted to Prof. Dr. Yonca Karagül YÜCEER for her support and permitting our access to her laboratory infrastructure.
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Year 2025,
Volume: 31 Issue: 1, 22 - 32, 14.01.2025
Buket Aydeniz-güneşer
,
Onur Güneşer
,
Meral Kekeçoğlu
,
Sevgi Kolaylı
References
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- Al-Ghamdi A A, Al-Khaibari A M & Omar M O M (2011). Effect of honeybee race and worker age on development and histological structure of hypopharyngeal glands of honeybee. Saudi Journal of Biological Sciences 18(2): 113-116. https://doi.org/10.1016/j.sjbs.2011.01.001
- Ali E M (2014). Contributions of some biological activities of honey bee venom. Journal of Apicultural Research 53(4): 441-451. https://doi.org/10.3896/IBRA.1.53.4.13
- Ali H, Alqarni A S, Iqbal J, Owayss A A, Raweh H S & Smith B H (2019). Effect of season and behavioral activity on the hypopharyngeal glands of three honeybee Apis mellifera L. races under stressful climatic conditions of central Saudi Arabia. Journal of Hymenoptera Research 68: 85-101. https://doi.org/doi: 10.3897/jhr.68.29678
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Ando H, Kurata A & Kishimoto N (2015). Antimicrobial properties and mechanism of volatile isoamyl acetate, a main flavor component of Japanese sake (Ginjo‐shu). Journal of Applied Microbiology 118(4): 873-880. https://doi.org/10.1111/jam.12764
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- El-Didamony S E, Amer R I & El-Osaily G H (2022). Formulation, characterization and cellular toxicity assessment of a novel bee-venom microsphere in prostate cancer treatment. Scientific Reports 12(1): 13213. https://doi.org/10.1038/s41598-022-17391-w
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- El Sharkawi F Z, Saleh S S & El Sayed A F M (2015). Potential anti cancer activity of snake venom, bee venom and their components in liver and breast carcinoma. International Journal of Pharmaceutical Sciences and Research 6(8): 3224. https://doi.org/10.13040/IJPSR.0975-8232
- Flanjak I, Kovačić M, Primorac L, Soldić A, Puškadija Z & Rajs B B (2021). Comparison between the quantity and quality of honey bee venom collected in the front and inside of the hive. Journal of Apicultural Research pp. 1-6. https://doi.org/10.1080/00218839.2021.1994262
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- Güneşer O & Yüceer Y K (2017). Biosynthesis of eight-carbon volatiles from tomato and pepper pomaces by fungi: Trichoderma atroviride and Aspergillus sojae. Journal of Bioscience and Bioengineering 123(4): 451-459. https://doi.org/10.1016/j.jbiosc.2016.11.013
- Huigens III R W, Brummel B R, Tenneti S, Garrison A T & Xiao T (2022). Pyrazine and phenazine heterocycles: Platforms for total synthesis and drug discovery. Molecules 27(3): 1112. https://doi.org/10.3390/molecules27031112
- Hussein A, El-Ansari M & Zahra A (2019). Effect of the honeybee hybrid and geographic region on the honeybee venom production. Journal of Plant Protection and Pathology 10(3): 171–176. https://doi.org/10.21608/jppp.2019.40922
- Hwang D S, Kim S K & Bae H (2015). Therapeutic effects of bee venom on immunological and neurological diseases. Toxins 7(7): 2413-2421. https://doi.org/10.3390/toxins7072413
- Isidorov V, Zalewski A, Zambrowski G & Swiecicka I (2023). Chemical Composition and Antimicrobial Properties of Honeybee Venom. Molecules 28(10): 4135. https://doi.org/10.3390/ molecules28104135
- Jeleń H & Gracka A (2016). Characterization of aroma compounds: Structure, physico‐chemical and sensory properties. In E. Guichard, C. Salles, M. Morzel, & AM. Le Bon (Eds.), Flavour: from food to perception (pp. 126-153). John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118929384.ch6
- Jung G B, Huh J E, Lee H J, Kim D, Lee G J Park H K & Lee J D (2018). Anti-cancer effect of bee venom on human MDA-MB-231 breast cancer cells using Raman spectroscopy. Biomedical Optics Express 9(11): 5703-5718. https://doi.org/10.1364/BOE.9.005703
- Kaziur-Cegla W, Jochmann M A, Molt K, Bruchmann A & Schmidt T C (2022). In-tube dynamic extraction for analysis of volatile organic compounds in honey samples. Food Chemistry X, 14, Article 100337. https://doi.org/10.1016/j.fochx.2022.100337
- Kekeçoğlu M, Çaprazlı T, Samancı A E T, Samancı T & Önder E Y (2022). Factors affecting quality of honeybee venom. Journal of Apicultural Science 66(1): 5-14. https://doi.org/10.2478/jas-2022-0001
- Kekecoglu M, Sonmez E, Acar M K & Karaoglu S A (2021). Pollen analysis, chemical composition and antibacterial activity of Anatolian chestnut propolis collected from Yıgılca region. Biology Bulletin 48(7): 21-728. https://doi.org/10.1134/S106235902106011X
- Kumar N R, Devi A, Kriti H & Kriti N (2014). Comparative biochemical studies on the poison glad and poison sac of the worker bees of three different apis species (Apis dorsata, Apis mellifera and Apis florea). International Journal of Therapeutic Applications 16: 8–16
- Kumar S & Aggarwal R (2019). Thiazole: A privileged motif in marine natural products. Mini-Reviews in Organic Chemistry 16(1): 26-34. https://doi.org/10.2174/1570193X15666180412152743
- Maga J A & Sizer C E (1973). Pyrazines in foods. Review. Journal of Agricultural and Food Chemistry 21(1): 22-30.https://doi.org/10.1021/jf60185a006
- Melda A, Kalaycioğlu Z, Kolayli S & Berker B (2021). Comparative determination of melittin by capillary electrophoretic methods. Journal of the Turkish Chemical Society Section A: Chemistry 8(4): 1211-1216. https://doi.org/10.18596/jotcsa.949188
- Miguel M G & Antunes M D (2011). Is propolis safe as an alternative medicine? Journal of Pharmacy and Bioallied Sciences 3(4): 479-495. https://doi.org/10.4103/0975-7406.90101
- Mortzfeld F B, Hashem C, Vranková K, Winkler M & Rudroff F (2020). Pyrazines: Synthesis and industrial application of these valuable flavor and fragrance compounds. Biotechnology Journal 15(11): 2000064. https://doi.org/10.1002/biot.202000064
- Ouradi H, Hanine H, Fauconnier M L, Kenne T, Rizki H, Ennahli S & Hssaini L (2021). Determination of physico-biochemical proprieties and composition in volatile constituents by solid phase micro-extraction of honey samples from different botanical and geographical origins in Morocco. Journal of Apicultural Research 60(1): 84-98. https://doi.org/10.1080/00218839.2020.1718339
- Özkök A (2018). Türkiye’de hızla büyüyen sektör: Arı ürünlerine genel bir bakış. Palme Yayın Dağıtım.
Paulino B N, Silva G N, Araújo F F, Néri-Numa I A, Pastore G M, Bicas J L & Molina G (2022). Beyond natural aromas: The bioactive and technological potential of monoterpenes. Trends in Food Science & Technology 128: 188-201
- Pawliszyn J (1999). Quantitative aspects of SPME. In: J. Pawliszyn (Ed.), Applications of solid phase microextraction (pp. 3-21). Royal Society of Chemistry. https://doi.org/10.1039/9781847550149
- Ramos O Y, Salomón V, Libonatti C, Cepeda R, Maldonado L & Basualdo M (2018). Effect of botanical and physicochemical composition of Argentinean honeys on the inhibitory action against food pathogens. LWT 87: 457-463. https://doi.org/10.1016/j.lwt.2017.09.014.
- Rockwood G A, Thompson D E & Petrikovics I (2016). Dimethyl trisulfide: A novel cyanide countermeasure. Toxicology & Industrial Health, 32(12): 2009-2016. https://doi.org/10.1177/0748233715622713
- Salehi B, Upadhyay S, Erdogan Orhan I, Kumar Jugran A, Jayaweera L D S A, Dias D, Sharopov F, Taheri Y, Martins N, Baghalpour N, Cho W C & Sharifi-Rad J (2019). Therapeutic potential of α-and β-pinene: A miracle gift of nature. Biomolecules 9(11): 738. https://doi.org/10.3390/biom9110738
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