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Ancient DNA

Year 2015, Issue: 30, 53 - 60, 30.12.2015
https://doi.org/10.1501/antro_0000000320

Abstract

DNA studies from highly degraded biological samples that are not preserved in suitable conditions for DNA analyses are called “ancient DNA analyses” Ancient DNA studies may shed light on many scientific issues in parallel with the improvements of the field of molecular biology, however it requires a higher level of sensitivity during applications when compared to standard molecular genetic studies. These researches raises the need for a collaborative work of scientists from different disciplines to carry out several explorative stages; initiating from sample collection to laboratory analyses which finally provides solutions on the evolutionary and anthropological hypothesis. The aim of this article is to focus on the importance of ancient DNA studies, discuss the problems encountered during these studies and provide solution proposals to overcome these problems

References

  • Alakoc YD., Aka PS. (2009) “Orthograde Entrance Technique To Recover DNA From Ancient Teeth Preserving The Physical Structure.” Forensic Sci Int. 2009 Jul 1;188(1-3):96-8.
  • Alakoc, Y. D., Aka P. S., , Eğin Y, Akar N. (2010). "V Leiden in an Urartian, dating Back to 1000 BC”. Journal of the International Academy of Clinical and Applied Thrombosis/ Hemostasis (Clin Appl. Tromb Hemost) 16 (6):679-83.
  • Bouwman, A. S., Chilvers E. R., Brown K. A., Brown T. A. (2006). "Brief communication: identification of the authentic ancient DNA sequence in a human bone contaminated with modern DNA." Am J Phys Anthropol 131(3): 428-31.
  • Germonpore M., Sablin M.V. et al. (2009).” Fossil dogs wolvesfrom Palaolithic sites in Belgium, the Ukraine and Russia: osteometry, ancient DNA and stable isotopes” Journal od Archaelogical Science 36:473-490.
  • Halle J., Froese GG, et al (2009). “Ancient DNA reveals late survival of mammoth and horse in interior Alaska” PNAS 106 (52): 22352-57.
  • Higgins D., Austin J. (2013). “ Teeth as a source of DNA for forensic identification of human remains”.Science and Justice. 53:413-441.
  • Higuchi, R., B. Bowman, et al. (1984). "DNA Squences from quagga, an extinct member of the horse family." Nature 312: 282-284.
  • Hummel S. (2007). “Ancient DNA” . Handbook of Paleoantropology. Springer: 643-671.
  • Kirsanow K. ve Burger J. (2012). “Ancient human DNA” Annals of Anatomy 194: 121-132.
  • Meyer, E., M. Wiese, et al. (2000). "Extraction and amplification of authentic DNA from ancient human remains." Forensic Sci Int 113(1-3): 87-90.
  • Pääbo S. (1985). “Molecular Colning of ancient Egyptian mummy DNA” Nature. 314: 644-645.
  • Pruvost, M., R. Schwarz, et al. (2007). "Freshly excavated fossil bones are best for amplification of ancient DNA." Proc Natl Acad Sci U S A 104(3): 739-44.
  • Rohland, N. and M. Hofreiter (2007). "Ancient DNA extraction from bones and teeth." Nat Protoc 2(7): 1756-62.
  • Schubert, M., Ginolhac A., Thompson J., Al- Rashid K., Willerslev E., Krogh A. and Orlando L. (2012) “Improving ancient DNA read mapping aganist modern genomes”. BMC Genomics: 13:178.
  • Von Wurmb-Schwark, N., M. Harbeck, et al. (2003). "Extraction and amplification of nuclear and mitochondrial DNA from ancient and artificially aged bones." Leg Med (Tokyo) 5 Suppl 1: S169-72.
  • Yang, DY. ve Watt, K. (2005). "Contamination controls when preparing archaeological remains for ancient DNA analysis." Journal of Archaeological Sceince 32: 331-336.

Antik DNA Çalışmaları ve Karşılaşılan Sorunlar

Year 2015, Issue: 30, 53 - 60, 30.12.2015
https://doi.org/10.1501/antro_0000000320

Abstract

İleri derecede zarar görmüş ve DNA eldesi için özel koşullarda saklanmamış biyolojik örneklerden DNA elde edilerek gerçekleştirilen moleküler genetik çalışmaları “antik DNA çalışmaları” olarak adlandırılır. Moleküler biyoloji alanında ortaya konulan gelişmeler paralelinde pek çok bilimsel soruna ışık tutacak olan antik DNA çalışmaları, uygulamada standart moleküler genetik çalışmalarına kıyasla farklı hassasiyetler gerektirmektedir. Örneklerin elde edilmesinden laboratuvarda gerçekleştirilen birçok aşamaya kadar pek çok farklı bilim dalından insanın ortak çalışmasını gerektiren bu araştırmalar, sonunda evrimsel ve antropolojik sorulara çözüm sunacak güçtedir. Bu makalede antik DNA çalışmalarının önemi ve çalışma süresince karşılaşılan sorunlar ve çözüm önerileri üzerinde durulacaktır

References

  • Alakoc YD., Aka PS. (2009) “Orthograde Entrance Technique To Recover DNA From Ancient Teeth Preserving The Physical Structure.” Forensic Sci Int. 2009 Jul 1;188(1-3):96-8.
  • Alakoc, Y. D., Aka P. S., , Eğin Y, Akar N. (2010). "V Leiden in an Urartian, dating Back to 1000 BC”. Journal of the International Academy of Clinical and Applied Thrombosis/ Hemostasis (Clin Appl. Tromb Hemost) 16 (6):679-83.
  • Bouwman, A. S., Chilvers E. R., Brown K. A., Brown T. A. (2006). "Brief communication: identification of the authentic ancient DNA sequence in a human bone contaminated with modern DNA." Am J Phys Anthropol 131(3): 428-31.
  • Germonpore M., Sablin M.V. et al. (2009).” Fossil dogs wolvesfrom Palaolithic sites in Belgium, the Ukraine and Russia: osteometry, ancient DNA and stable isotopes” Journal od Archaelogical Science 36:473-490.
  • Halle J., Froese GG, et al (2009). “Ancient DNA reveals late survival of mammoth and horse in interior Alaska” PNAS 106 (52): 22352-57.
  • Higgins D., Austin J. (2013). “ Teeth as a source of DNA for forensic identification of human remains”.Science and Justice. 53:413-441.
  • Higuchi, R., B. Bowman, et al. (1984). "DNA Squences from quagga, an extinct member of the horse family." Nature 312: 282-284.
  • Hummel S. (2007). “Ancient DNA” . Handbook of Paleoantropology. Springer: 643-671.
  • Kirsanow K. ve Burger J. (2012). “Ancient human DNA” Annals of Anatomy 194: 121-132.
  • Meyer, E., M. Wiese, et al. (2000). "Extraction and amplification of authentic DNA from ancient human remains." Forensic Sci Int 113(1-3): 87-90.
  • Pääbo S. (1985). “Molecular Colning of ancient Egyptian mummy DNA” Nature. 314: 644-645.
  • Pruvost, M., R. Schwarz, et al. (2007). "Freshly excavated fossil bones are best for amplification of ancient DNA." Proc Natl Acad Sci U S A 104(3): 739-44.
  • Rohland, N. and M. Hofreiter (2007). "Ancient DNA extraction from bones and teeth." Nat Protoc 2(7): 1756-62.
  • Schubert, M., Ginolhac A., Thompson J., Al- Rashid K., Willerslev E., Krogh A. and Orlando L. (2012) “Improving ancient DNA read mapping aganist modern genomes”. BMC Genomics: 13:178.
  • Von Wurmb-Schwark, N., M. Harbeck, et al. (2003). "Extraction and amplification of nuclear and mitochondrial DNA from ancient and artificially aged bones." Leg Med (Tokyo) 5 Suppl 1: S169-72.
  • Yang, DY. ve Watt, K. (2005). "Contamination controls when preparing archaeological remains for ancient DNA analysis." Journal of Archaeological Sceince 32: 331-336.
There are 16 citations in total.

Details

Primary Language Turkish
Subjects Anthropology, Archaeology
Other ID JA87NS27TZ
Journal Section Review Articles
Authors

H. Merve İyras This is me

Yeşim Doğan This is me

Publication Date December 30, 2015
Submission Date August 1, 2015
Acceptance Date December 1, 2015
Published in Issue Year 2015 Issue: 30

Cite

APA İyras, H. M., & Doğan, Y. (2015). Antik DNA Çalışmaları ve Karşılaşılan Sorunlar. Antropoloji(30), 53-60. https://doi.org/10.1501/antro_0000000320

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