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Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi

2146-1880 2146-698X

Artvin Çoruh University

10.17474/artvinofd.702853

Structural Biology

Yapısal Biyoloji

In vitro antimicrobial, antioxidant activity and chemical characterization of Hypericum perforatum L. essentıal oil

Hyperıcum perforatum L. esansiyel yağının in vitro antimikrobiyal, antioksidan aktivite ve kimyasal karakterizasyonu

https://orcid.org/0000-0001-5837-6893

Ertürk

Ömer

ORDU ÜNİVERSİTESİ

https://orcid.org/0000-0001-6349-2346

Aydın

Gülçin

ordu üniversitesi

https://orcid.org/0000-0001-5155-5784

Çol Ayvaz

Melek

ORDU ÜNİVERSİTESİ

09 15 2020

21 2 330 339 03 12 2020 09 02 2020

2000

Artvin Coruh University Journal of Forestry Faculty

In vitro antimicrobial and antioxidant activities and chemical composition of extracts prepared using two different solvents, hexane, ethanol, of commercially available essential oil sample isolated from Hypericum perforatum L. were determined. St. John's Wort essential oil was examined for various bacteria and fungi by using disc diffusion method to test its antimicrobial effectiveness. The chemical content of H. perforatum essential oil was determined by GC/MS method. It was concluded that hexane extract is more effective than ethanol extract against all bacterial and fungal strains. St. John's Wort oil had higher antimicrobial effects against fungi than bacteria. However, compared to standard antibiotics, activity is lower in the case of both ethanol and hexane extracts. The antioxidant activity of the 2% essential oil sample was also evaluated using 3 different methods and it was concluded that the oil sample's effectiveness of sweeping the DPPH radical was more effective than the other methods tested. With the chemical characterization as a result of GC/MS analysis 60 different compounds were identified including mainly carvacrol (4.78%), decane (8.15%), nonane (7.63%), p-cymene (9.04%), limonene (15.04%), α,p- dimethylstyrene (11.80%), camphor (4.21%), Isoborneol (%7.77) according to their highest area (%).

Ticari olarak temin edilen Hypericum perforatum L.’den izole edilmiş olan esansiyel yağ örneğinin hekzan ve etanol olmak üzere iki farklı çözgen kullanılarak hazırlanan ekstraktlarının in vitro antimikrobiyal ve antioksidan aktiviteleri ve kimyasal kompozisyonu belirlendi. Sarı kantaron esansiyel yağı antimikrobiyal etkinliğinin test edilebilmesi amacıyla disk difüzyon yöntemi kullanılarak bazı bakteri ve mantar türlerine karşı incelendi. H. perforatum esansiyel yağının kimyasal içeriği ise GC/MS yöntemi ile belirlendi. Tüm bakteri ve mantar suşlarına karşı hekzan özütünün etanol ekstraktına nazaran daha etkili olduğunu sonucuna varıldı. Sarı kantaron yağı funguslara karşı bakterilerden daha yüksek antimikrobiyal etki gösterdi. Ancak standart antibiyotiklerle karşılaştırıldığında hem etanol hem de hekzan ekstraktları durumunda aktivite daha düşüktür. esansiyel yağ %2 oranındaki numunesinin antioksidan aktivitesi de 3 farklı yöntem kullanılarak değerlendirilmiş ve örneğinin DPPH radikalini süpürme etkinliğinin test edilen diğer metotlara göre daha etkili olduğu sonucuna ulaşıldı. Yapılan kimyasal karakterizasyon çalışmalarıyla GC/MS analizleri sonucunda alan değeri (%) en yüksek olan bileşikler başlıca %4.78 karvakrol, %8.15 dekan, %7.63 nonan, %9.04 p-simen, %15.04 limonen, %11.80 alpha-para-dimetilstiren, %4.21 kamfor ve %7.77 isoborneol olmak üzere 60 farklı bileşik tanımlandı.

antimikrobiyoloji antioxsidant Hypericum perforatum

Hypericum perforatum Antimicrobial Antioxidant

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