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KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU

Year 2011, Volume: 26 Issue: 2, 1 - 12, 20.02.2013

Tülay Akaylı , Çiğdem Ürkü Buket Başaran

Abstract

Bu çalışma ile Stafilokokkozis'in klinik bulgularını gösteren kültür gökkuşağı alabalıkları (Oncornhychus mykiss) ve sinarit (Dentex dentex) balıklarında ölümlere neden olan hastalık etkeninin saptanması amaçlanmıştır. Klinik bulgu olarak letarji, renkte koyulaşma, yüzgeç diplerinde ve gözlerde hemoraji gibi belirtiler gösteren enfekte balıklardan bakteriyolojik inceleme amacıyla TSA (Tryptic Soy Agar) ve T-TSA (%1 NaCl içeren Tryptic Soy Agar) besiyerlerine ekimler yapılmıştır. Çalışma sonucunda hasta balıklardan Gram-pozitif kok, fermantatif, hareketsiz, sitokrom oksidaz ve koagülaz negatif, katalaz pozitif 8 izolat elde edilmiş ve konvansiyonel yöntem ve hızlı tanı kiti (API STAPH) sonuçlarına göre izole edilen bakterinin Staphylococcus cohnii subsp. cohnii olduğu belirlenmiştir. Ayrıca hasta balıkların bazılarında bu etken ile birlikte karışık enfeksiyonlarda tespit edilmiş ve izolatlar arasında antibiyogram duyarlılık testinde farklılıklar gözlenmiştir.

Yurdumuzda daha önce yapılan çalışmalarda kültürü balıklarından farklı Staphylococcus türleri rapor edilmiş olmakla birlikte bu çalışma ile yeni bir tür olarak S.cohnii subsp. cohnii ilk kez tespit edilmiştir.

Keywords

Stafılokokkozis Staphylococcus cohnii subsp

References

  • Alpbaz A.G., (2005). Deniz Balıkları Yetiştiriciliği, Ege Üniversitesi Basımevi Bornova- İzmir.
  • Austin, B. ve Austin, D.A., (2007). Bacterial Fish Pathogens Disease of Farmed and Wild Fish, Third Edition, Ellis Horwood, London, UK, pp 376.
  • Athanassopoulou F., Prapas T.H. ve Rodger H., (1999). Diseases of Puntazzo puntazzo Cuvier in marine aquacultura systems in Greece, Journal of Fish Diseases, 22, 215-218.
  • Bascomb, S. ve Manafı, M., (1998). Use of enzyme tests in characteriza- tion and identification of aerobic and facultatively anaerobic gram-positive cocci. Clinical Microbiology Reviews, 11, 318-340.
  • Behme, R.J., Shuttleworth, R., Mcnabb, A. ve Colby,W.D., (1996). Identifıcation of Staphylococci With a self-educating system using fatty 11 acid analysis and biochemical tests, Journal of Clinical Microbiology, 34, (12), 3075-3084.
  • Buxton, A. ve Fraser, G., (1977). Animal Microbiology, Volume:1 Immu- nology, Bacteriology, Mycology, Disease of Fish and Laboratory Methods, Blackwell Science Publishing, London.
  • Götz, F., Bannerman,T. ve Schleifer, K.S., (2006). The Genera Staphy- lococcus and Macrococcus .In.‘ Prokaryotes Chapter1.2.1, 4:5—75 .
  • Huan, S.H., Chen,W.C., Shei, M.C., Liao, I.C. ve Chen, S.N., (1999). Studies on epizootiology and Pathogenicity of Staphylococcus epidermidis in Tilapia (Oreochromis spp.) Cultured in Taiwan. Zoological Studies, 38, 2, 178- 1 88.
  • Kloss W.E. ve Wolfshohl, J .F., (1991). Staphylococcus cohnii subspe- cies: Staphylococcus cohnii subsp. cohnii subsp. nov. and Staphylococcus cohnii subsp. urealyticum subsp. nov. International Journal of Systematic Bacteriology, 41, 2, 284-289.
  • Kloos, W.E. ve Bannerrnan, T., (1995). Staphylococcus and Micrococcus. In: Mray PR, Baron EJ, Fallew MA (ed). Manual of Clinical Microbiology. 6th ed. ASM, 282-298.
  • Konemann, E.W., (1992), Color Atlas and Diagnostic Microbiology, ISBN 0-1234455-3, 258-125.
  • Kubilay, A. ve Uluköy, G., (2004). First isolation of Staphylococcus epidermidis from cultured gilthead sea bream (Sparus aurata, L.1758) in Turkey. Bulletin European Fish Pathology, 24, 3 ,137-143.
  • Kusuda, R.,Sugiyama, A., (1981). Studies on the characters of Staphylo- coccus epidermidis isolated from diseased Şshes-I. On the morphological, biological and biochemical properties, Fish Pathology,l6:15-24.
  • MousaVi,S.S., Khara, H., Saeidi, A.A., Ghiasi, M. ve Zahedi, A., (2010). Determination of Staphylococcosis and Micrococcosis outbreak on selected rainbow trout farms in Mazandaran province, Journal of Fisheries, 4,. l.
  • Plumb, J.A. ve Bowser, P.R., (1983). Microbial Fish Disease Laboratory Manual. Brown Printing Company, Albana.
  • Rozalska, M. ve Szewczyk EM. (2008): Staphylococcus cohnii Hemoly- sins — Isolation, PuriŞcation and Properties.Folia Microbiol. 53 (6), 521—526.
  • Shah, K.L. ve Tyagi, B.C., (1986). An eye disease in silver carp, Hypoph- thalmichthys molitrix, held in tropical ponds, associated With the bacterium Staphylococcus aureus. Aquaculture, 55, l,l-4.
  • Timur, G. ve Akayli, T., (2003). First study of Staphylococcosis in farmed rainbow trout fry (Oncorhyncus mykiss W. 1792) in Turkey”. International Symposium of Fisheries and Zoology, October 23 -26, İstanbul, 67-80.
  • Timur, G.,Timur M., Akayli, T., Korun, J ., (2007). A survey study of pathologies affecting farmed sea bass (Dicentrarcus labrax) and marine cultured rainbow trout (O.mykiss) in Turkey. 13th international conference of the EAFP on Diseases of Fish and ShellŞsh, 17-22 September 2007 Grado-Italy, Book of Abstracts, 308.
  • Varvarigos, P., (2001).Gram positive cocco-bacteria (Micrococcaceae, Streptococcaceae) causing systemic disease to intensively farmed marine Şsh in the Mediterranean. A brief review. http://Www.vetcare.gr/Gram_posi- tive_cocci.htm.
  • Whitman, K. A., (2004). FinŞsh and ShellŞsh Bacteriology Manual Techniques and Procedures, ABlackwell Publishing Company, ISBN 0- 8138-1952-0.

Year 2011, Volume: 26 Issue: 2, 1 - 12, 20.02.2013

Tülay Akaylı , Çiğdem Ürkü Buket Başaran

Abstract

References

  • Alpbaz A.G., (2005). Deniz Balıkları Yetiştiriciliği, Ege Üniversitesi Basımevi Bornova- İzmir.
  • Austin, B. ve Austin, D.A., (2007). Bacterial Fish Pathogens Disease of Farmed and Wild Fish, Third Edition, Ellis Horwood, London, UK, pp 376.
  • Athanassopoulou F., Prapas T.H. ve Rodger H., (1999). Diseases of Puntazzo puntazzo Cuvier in marine aquacultura systems in Greece, Journal of Fish Diseases, 22, 215-218.
  • Bascomb, S. ve Manafı, M., (1998). Use of enzyme tests in characteriza- tion and identification of aerobic and facultatively anaerobic gram-positive cocci. Clinical Microbiology Reviews, 11, 318-340.
  • Behme, R.J., Shuttleworth, R., Mcnabb, A. ve Colby,W.D., (1996). Identifıcation of Staphylococci With a self-educating system using fatty 11 acid analysis and biochemical tests, Journal of Clinical Microbiology, 34, (12), 3075-3084.
  • Buxton, A. ve Fraser, G., (1977). Animal Microbiology, Volume:1 Immu- nology, Bacteriology, Mycology, Disease of Fish and Laboratory Methods, Blackwell Science Publishing, London.
  • Götz, F., Bannerman,T. ve Schleifer, K.S., (2006). The Genera Staphy- lococcus and Macrococcus .In.‘ Prokaryotes Chapter1.2.1, 4:5—75 .
  • Huan, S.H., Chen,W.C., Shei, M.C., Liao, I.C. ve Chen, S.N., (1999). Studies on epizootiology and Pathogenicity of Staphylococcus epidermidis in Tilapia (Oreochromis spp.) Cultured in Taiwan. Zoological Studies, 38, 2, 178- 1 88.
  • Kloss W.E. ve Wolfshohl, J .F., (1991). Staphylococcus cohnii subspe- cies: Staphylococcus cohnii subsp. cohnii subsp. nov. and Staphylococcus cohnii subsp. urealyticum subsp. nov. International Journal of Systematic Bacteriology, 41, 2, 284-289.
  • Kloos, W.E. ve Bannerrnan, T., (1995). Staphylococcus and Micrococcus. In: Mray PR, Baron EJ, Fallew MA (ed). Manual of Clinical Microbiology. 6th ed. ASM, 282-298.
  • Konemann, E.W., (1992), Color Atlas and Diagnostic Microbiology, ISBN 0-1234455-3, 258-125.
  • Kubilay, A. ve Uluköy, G., (2004). First isolation of Staphylococcus epidermidis from cultured gilthead sea bream (Sparus aurata, L.1758) in Turkey. Bulletin European Fish Pathology, 24, 3 ,137-143.
  • Kusuda, R.,Sugiyama, A., (1981). Studies on the characters of Staphylo- coccus epidermidis isolated from diseased Şshes-I. On the morphological, biological and biochemical properties, Fish Pathology,l6:15-24.
  • MousaVi,S.S., Khara, H., Saeidi, A.A., Ghiasi, M. ve Zahedi, A., (2010). Determination of Staphylococcosis and Micrococcosis outbreak on selected rainbow trout farms in Mazandaran province, Journal of Fisheries, 4,. l.
  • Plumb, J.A. ve Bowser, P.R., (1983). Microbial Fish Disease Laboratory Manual. Brown Printing Company, Albana.
  • Rozalska, M. ve Szewczyk EM. (2008): Staphylococcus cohnii Hemoly- sins — Isolation, PuriŞcation and Properties.Folia Microbiol. 53 (6), 521—526.
  • Shah, K.L. ve Tyagi, B.C., (1986). An eye disease in silver carp, Hypoph- thalmichthys molitrix, held in tropical ponds, associated With the bacterium Staphylococcus aureus. Aquaculture, 55, l,l-4.
  • Timur, G. ve Akayli, T., (2003). First study of Staphylococcosis in farmed rainbow trout fry (Oncorhyncus mykiss W. 1792) in Turkey”. International Symposium of Fisheries and Zoology, October 23 -26, İstanbul, 67-80.
  • Timur, G.,Timur M., Akayli, T., Korun, J ., (2007). A survey study of pathologies affecting farmed sea bass (Dicentrarcus labrax) and marine cultured rainbow trout (O.mykiss) in Turkey. 13th international conference of the EAFP on Diseases of Fish and ShellŞsh, 17-22 September 2007 Grado-Italy, Book of Abstracts, 308.
  • Varvarigos, P., (2001).Gram positive cocco-bacteria (Micrococcaceae, Streptococcaceae) causing systemic disease to intensively farmed marine Şsh in the Mediterranean. A brief review. http://Www.vetcare.gr/Gram_posi- tive_cocci.htm.
  • Whitman, K. A., (2004). FinŞsh and ShellŞsh Bacteriology Manual Techniques and Procedures, ABlackwell Publishing Company, ISBN 0- 8138-1952-0.
There are 21 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Tülay Akaylı

Çiğdem Ürkü This is me

Buket Başaran This is me

Publication Date February 20, 2013
Submission Date February 20, 2013
Published in Issue Year 2011 Volume: 26 Issue: 2

Cite

APA Akaylı, T., Ürkü, Ç., & Başaran, B. (2013). KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU. Aquatic Sciences and Engineering, 26(2), 1-12.
AMA Akaylı T, Ürkü Ç, Başaran B. KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU. Aqua Sci Eng. February 2013;26(2):1-12.
Chicago Akaylı, Tülay, Çiğdem Ürkü, and Buket Başaran. “KÜLTÜR BALIKLARINDA Staphylococcus Cohnii Subsp. Cohnii ENFEKSİYONU”. Aquatic Sciences and Engineering 26, no. 2 (February 2013): 1-12.
EndNote Akaylı T, Ürkü Ç, Başaran B (February 1, 2013) KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU. Aquatic Sciences and Engineering 26 2 1–12.
IEEE T. Akaylı, Ç. Ürkü, and B. Başaran, “KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU”, Aqua Sci Eng, vol. 26, no. 2, pp. 1–12, 2013.
ISNAD Akaylı, Tülay et al. “KÜLTÜR BALIKLARINDA Staphylococcus Cohnii Subsp. Cohnii ENFEKSİYONU”. Aquatic Sciences and Engineering 26/2 (February 2013), 1-12.
JAMA Akaylı T, Ürkü Ç, Başaran B. KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU. Aqua Sci Eng. 2013;26:1–12.
MLA Akaylı, Tülay et al. “KÜLTÜR BALIKLARINDA Staphylococcus Cohnii Subsp. Cohnii ENFEKSİYONU”. Aquatic Sciences and Engineering, vol. 26, no. 2, 2013, pp. 1-12.
Vancouver Akaylı T, Ürkü Ç, Başaran B. KÜLTÜR BALIKLARINDA Staphylococcus cohnii subsp. cohnii ENFEKSİYONU. Aqua Sci Eng. 2013;26(2):1-12.

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