Atatürk Üniversitesi Veteriner Bilimleri Dergisi 1306-6137 2147-9615 Ataturk University Cronobacter sakazakii’nin Gıda Mikrobiyolojisindeki Önemi Güner Ahmet Telli Nihat 12 21 2011 6 3 251 263 01 13 2011 Copyright © 2006, Atatürk University Journal of Veterinary Sciences 2006 Atatürk University Journal of Veterinary Sciences

Cronobacter sakazakii birçok yaş grubunda farklı enfeksiyonlara (örn., pnömoni, konjuktivitis, apandisit) sebep olmakla birlikte, özellikle yeni doğan bebeklerde ve çocuklarda menenjit, nekrotik enterokolitis ve bakteriyemiye neden olan bir patojendir. Toz bebek mamalarının tüketimine bağlı olarak son yıllarda yaşanan sağlık sorunları, dünya genelinde toz bebek mamaları üzerine artan sayıda araştırmalar yapılmasına neden olmuştur. Amerikan Gıda ve İlaç Dairesi (FDA), Amerika Birleşik Devletleri’ndeki sağlık kuruluşlarını, toz bebek mamaları ile beslenen yeni doğan bebeklerde C. sakazakii’den kaynaklanan enfeksiyonun varlığı hakkında uyarmıştır. Toz bebek mamaları üzerinde yoğunlaşan uluslar arası hassasiyet üzerine, 2004 yılında Gıda ve Tarım Örgütü ve Dünya Sağlık Örgütü (FAO/WHO) neonatal enfeksiyonlar ve toz bebek mamaları ile ilişkili mikroorganizmalar konusunda bir risk yaklaşımı görevini üstlenmişler ve Cronobacter spp ve Salmonella spp.’yi hijyen kalitesi olarak kategorize etmişlerdir. Bununla birlikte C. sakazakii, et, sebze, peynir, tohum, baharat olmak üzere birçok gıdada da tespit edilmiştir. Toz bebek formüllerinde uzun süre canlı kalabilmesinin yanı sıra, tahıl kökenli bebek devam mamalarının, bazı taze meyve ve sebzelerin C. sakazakii’nin üremesini desteklemesi ayrıca önem arz etmektedir. Fırsatçı bir patojen olan C. sakazakii’nin sebep olacağı gıda kaynaklı enfeksiyonlara karşı başlıca koruyucu önlemler, ısıl işlemlerde etkinliğin sağlanmasının yanı sıra, üretim yeri çevresinden ısıl işlem sonrası meydana gelebilecek kontaminasyonları ortadan kaldırma yönünde olmalıdır.

 Besin Mikrobiyolojisi Cronobacter sakazakii Güvenli Gıda Al-Holy MA., Lin M., Al-Qadiri HM., Rasco, BA., 2008. A comparative study between overlay method and selective-differential media for recovery of stressed Enterobacter sakazakii cells from infant formula. Food Microbiol., 25, 22-28. Al-Nabulsi A., Osaili TM., Al-Holy MA., Shaker RR., Ayyash MM., Olaimat AN., Holley, RA., 2009. Influence of desiccation on the sensitivity of Cronobacter spp. to lactoferrin or nisin in broth and powdered infant formula. Int. J. Food Microbiol., 136, 221-226. Arroyo C., Condon S., Pagan R., 2009. Thermobacteriological Enterobacter sakazakii. Int J Food Microbiol., 136, 110-118. characterization of Beuchat LR., Kim H., Gurtler JB., Lin LC., Ryu JH., and Richards GM., 2009. Cronobacter sakazakii in foods and factors affecting its survival, groth and inactivation. Int. J. Food Microbiol., 136, 204-213. Biering G., Karlsson S, Clark NC., Jonsdottir KE., Ludvigsson P., Steingrimsson O., 1989. Three cases of neonatal meningitis caused by Enterobacter sakazakii in powdered milk. J. Clin. Microbiol., 27, 9, 2054-2056. Block C., Peleg O., Minster N., Bar-Oz B., Simhon A., Arad I., Shapiro M., 2002. Cluster of neonatal infections in Jerusalem due to unusual biochemical variant of Enterobacter sakazakii. Europ. J. Clin Microbiol. & Dis., 21, 8, 613-616. Bowen AB., Braden, CR., 2008. Enterobacter sakazakii disease and epidemiology. In: Enterobacter sakazakii, Ed. By: Jeffrey M. Farber, and Stephen J. Forsythe. Page 101-125, ASM Press, Washington. Burdette JH., Santos C., 2000. Enterobacter sakazakii brain abscess in the neonate: the importance of neuroradiologic imaging. Pediatr. Radiol., 30, 33-34. Clark NC., Hill BC., O’Hara CM., Steingrimsson O., Cooksey RC., 1990. Epidomiological typing of Enterobacter sakazakii in two neonatal nosocomial outbreaks. Diagn. Microbiol. Infect. Dis., 13, 467-472. Dauga C., Breeuwer P., 2008. Taxonomy and physiology of Enterobacter sakzakii. In: Enterobacter sakazakii, Ed By: Jeffrey M. Farber and Stephen J. Forsythe. Page 1-26, ASM Press, Washington. Druggan P., Iversen C., 2009. Culture media for the isolation of Cronobacter spp. Int J. Food Microbiol., 136, 169-178. Fanning S., Forsythe, S., 2008. Isolation and identification of Enterobacter sakazakii. In: Enterobacter sakazakii, Ed By: Jeffrey M. Farber and Stephen J. Forsythe. page 27-59, ASM Press, Washington. Farber JM., Pagotto F., Cordier JL., 2008. Regulatory aspects. In: Enterobacter sakazakii, Ed By: Jeffrey M. Farber and Stephen J. Forsythe. Page 235-253, ASM Press, Washington. Farmer JJ., Asbury MA., Hickman FW., Brenner DJ., 1980. The Enterobacteriaceae study group, Enterobacter sakazakii: A new species of Enterobacteriaceae isolated from clinical speciemens. Int. J. Syst. Bacteriol., 30, 3, 569-584. Farmer JJ., Davis BR., Hickman-Brenner FW., McWhorter A., Huntley-Carter JP., Asbury MA., Riddle C., Wathen-Grady HG., Elias C., Fanning JR., Steigerwalt AG., O’Hara CM., Morris GK., Smith PB., Brenner DJ., 1985. Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical speciemens. J. Clin. Microbiol., 21, 1, 46-76. FDA., 2002a. FDA warns about possible Enterobacter sakazakii infections in hospitalized newborns fed powdered infant formulas http:// www. cfsan. fda. gov /~lrd/ tpinf. html. April 12. FDA., 2002b. Isolation and enumeration of Enterobacter sakazakii from dehydrated powdered infant Formu- la. http:// www.cfsan.fda.gov /~com/ mmesakaz-. html. July 2002, Revised August 2002; Forsythe SJ., 2005. Enterobacter sakazakii and other bacteria in powdered infant milk formula. Matern. Child. Nutr., 1, 44-50. Gurtler JB., Kornacki JL., Beuchat LR., 2005. Enterobecter sakazakii: A coliform of increased concern to infant health. Int J. Food. Microbiol., 104, 1-34. Hawkins RE., Lissner CR., Sanford JP., 1991. Enterobacter sakazakii bacteremia in adult. South. Med. J., 84, 6, 793-795. Hoque A., Ahmed T., Shahidullah M., Hossain A., Mannan A., Noor K., Nahar K., Ilias M., Ahmed D., 2010. Isolation Cronobacter spp. from powdered infant Formula (PIF) in Bangladesh. Int. J. Food. Microbiol., 142, 375-378. identification of Hurrel E., Kucerova E., Loughlin M., Caubilla-Barron J., Forsythe SJ., 2009. Biofilm formation on enteral feeding tubes by Cronobacter sakazakii, Salmonella serovars and other Enterobacteriaceae. Int. J. Food Sci. Technol., 136, 227-231. ISO., 2006. Milk and Milk Products-Detection of Enterobacter sakazakii. Techincal Specification, ISO/TS 22964. Iversen C., Forsythe SJ., 2003. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci. Technol., 14, 443-454. Iversen C., Forsythe S., 2004. Isolation of Enterobacter sakazakii and other Enterobacteriaceae from powdered infant formula milk and related products. Food Microbiol., 21, 771-777. Iversen C., Druggan P., Forsythe SJ., 2004a. A selective differential medium for Enterobacter sakazakii. Int. J. Food Microbiol., 96, 133-139. Iversen C., Lane M., Forsythe SJ., 2004b. The growth profile, thermotolerance and biofilm formation of Enterobacter sakazakii grown in infant formula milk. Lett. Appl. Microbiol., 38, 378-382. Iversen C., Lehner A., Mullane N., Bidlas E., Cleenwerck I., Marugg J., Fanning S., Stephan R., Joosten H., 2007. The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii, comb. nov. Cronobacter sakazakii subsp. malonaticus, subsp. nov., Cronobacter dublinensis subsp. Joosten H., Marugg J., Stephan R., Klijn A., Jackson T., Iversen C., 2008. A rapid and reliable alternative to ISO Enterobacteriaceae. Int. J. Food Microbiol., 125, 344-346. for detection of Kandhai MC., Reij MW., Gorris LGM., Guillaume-Gentil O., Van Schothorst M., 2004a. Occurrence of Enterobacter environments and households. The Lancet, 363 (9402), 39-40. in food production Kandhai MC., Reij MW., van Puyvelde K., Guillaume-Gentil O., Beumer RR., van Schothorst M., 2004b. A new protocol for the detection of Enterobacter sakazakii applied to environmental samples. J. Food Protect., 67, 1267-1270. Kandhai MC., Heuvelink AE., Reij MW., Beumer RR., Dijk R., van Tilburg JJHC., van Schothorst M., Gorris LGM., 2010. A study into occurence of Cronobacter spp. in the Netherlands between 2001 and 2005. Food Cont., 21, 1127-1136. Lai KK., 2001. Enterobacter sakazakii infections among neonates, infants, childeren and adults. Medicine, 80, 2, 113-122. Lampel KA., Chen Y., 2009. Method for the isolation and detection of Enterobacter sakazakii (Cronobacter) from powdered infant formula. Int. J. Food. Microbiol., 136, 179-184. Leclercq A., Wanegue C., Baylac P., 2002. Comparision of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods. Appl. Environ. Microbiol, 68, 4, 1631-1638. Liu C., Zheng W., Zhang H., Hou Y., Liu Y., 2007. Sensitive and rapid detection of Enterobacter sakazakii in infant formula by loop-mediated isothermal amplification method. J. Food Safety, 29, 83-94. Molloy C., Cagney C., O’Brien S., Iversen C., Fanning S., Duffy G., 2009. Surveillance and characterisation by pulsed-field gel electrophoresis of Cronobacter spp. in farming and domestic environments, food production animals and retail foods. Int. J. Food Microbiol., 136, 198-203. Muytjens HL., Roelofs-Willemse H., Jaspar GHJ., 1988. Quality of powdered substitutes for breast milk with regard Enterobacteriaceae. J. Clin. Microbiol., 26, 4, 743- 746. of the family Nazarowec-White M., Farber JM., 1997a. Enterobacter sakazakii: A review. Int. J. Food Microbiol, 34, 103- 113. Nazarowec-White M., Farber JM., 1997b. Incidence, survival, and growth of Enterobacter sakazakii in infant formula. J. Food Protect., 60, 3, 226-230. Nazarowec-White M., Farber JM., 1997c. Thermal resistance of Enterobacter sakazakii in reconstituted dried-infant formula. Lett. Appl. Microbiol., 24, 9-13. Nazarowec-White M., Farber JM., 1999. Phenotypic and genotypic typing of food and clinical isolates of Enterobacter sakazakii. J. Med. Microbiol., 48, 559- 567. Oh SW., Kang DH., 2004. Flurogenic selective and differential medium for isolation of Enterobacter sakazakii. Appl. Environ. Microbiol., 70, 5692-5694. Osaili T., Forsythe S., 2009. Desiccation resistance and persistence of Cronobacter species in infant formula. Int. J. Food Microbiol., 136, 214-220. Pagotto F., Farber JM., Lenati R., 2008. Pathogenicity of Enterobacter sakazakii. In: Enterobacter sakazakii, Ed By: Jeffrey M. Farber, Stephen J. Forsythe. Page. 127-144, ASM Press, Washington. Reich F., König R., von Wiese W., Klein G., 2010. Prevalence of Cronobacter spp. in powdered infant formula processing environment. Int. J. Food Microbiol., 140, 214-217. Schmid M., Iversen C., Gontia I., Stephan R., Hofmann A., Hartmann A., Jha B., Eberl L., Riedel K., Lehner A., 2009. Evidence for a plant-associated natural habi- tat for Cronobacter spp. Res. Microbiol., 160, 608- 614. Skovgaard N., 2005. Current topics in food microbiology. Int. J. Food Microbiol., 99, 107-111. Simmons BP., Gelfand MS., Hass M., Metts L., Ferguson J., 1989. Enterobacter sakazakii infections in neonates associated with intrinsinc contamination of a powdered infant Formula. Infect. Cont. Hosp. Epidomiol., 10, 9, 398-401. Terragno R., Salve A., Pichel M., Epszteyn S., Brengi S., Binsztein N., 2009. Characterization and subtyping of Cronobacter spp. from imported powdered infant formulae in Argentina. Int. J. Food Microbiol., 136, 193-197. Towsend S., Forsythe SJ., 2008. The noenatal intestinal microbial flora, immunity, and infections. In: Enterobacter sakazakii, Ed By: Jeffrey M. Farber, Stephen J. Forsythe. Page 1-26, ASM Press, Washington. van Acker JV., Smet FD., Muyldermans G., Bougatef A., Naessens A., Lauwers S., 2001. Outbreak of necrotizing Enterobacter sakazakii in powdered milk formula. J. Clin. Microbiol., 39, 1, 293-297. associated with Wan-Ling H., Chang CH., Chou CC., 2010. Heat shock effects on the viability of Cronobacter sakazakii during the dehydration, fermentation, and storage of lactic culture milk products. Food Microbiol., 27, 280-285. Weber C., Stephan R., Druggan P., Joosten H., Iversen C., 2009. Improving the enrichment procedure for Enterobacteriaceae detection. Food Microbiol., 26, 565-572. Willis J., Robinson JE., 1988. Enterobacter sakazakii meningitis in neonates. Pediatr. Infect. Dis J., 7, 196- 199.