Atatürk Üniversitesi Veteriner Bilimleri Dergisi

1306-6137 2147-9615

Ataturk University

10.17094/ataunivbd.699247

Health Care Administration

Sağlık Kurumları Yönetimi

Asetamipridin Fare Kemik İliği Hücrelerinde Genotoksik etkisinin KA (Kromozomal Aberasyon) ve MN (Mikronükleus) Test Yöntemleri ile Araştırılması

Investigation of The Genotoxic Effect of Acetamiprid in Mouse Bone Marrow Cells by CA (Chromosomal Aberration) and MN (Micronucleus) Test Methods

https://orcid.org/0000-0002-0247-6462

Sandayuk

Şafak

KAFKAS ÜNİVERSİTESİ

https://orcid.org/0000-0002-3567-5775

Aksu Kılıçle

Pinar

KAFKAS ÜNİVERSİTESİ

10 27 2020

15 2 130 137 03 05 2020 04 24 2020

2006

Atatürk University Journal of Veterinary Sciences

Bu çalışma ile asetamipridin (ACE) mus musculus var. albino türü farelerin kemik iliği hücrelerinde kromozomal aberasyon(KA) ve mikronükleus (MN) analizi aracılığı ile genotoksik etkisinin belirlenmesini amaçladık. Fareler dört gruba ayrıldı. 1. grupnegatif kontrol grubu olarak belirlendi. 2. grup 5 mg/kg ACE grubu, 3. grup 10 mg/kg ACE grubu ve 4. grup, 15 mg/kg ACEgrubu olarak belirlendi ve ACE 14 gün boyunca oral olarak verildi. Femur hücrelerinde kromozomal aberasyon, mitotik indeks(MI) ve MN frekansları belirlendi. Sonuç olarak negatif kontrol grubuyla karşılaştırıldığında 5 mg/kg ve 10 mg/kg ACEuygulanan gruplarda genotoksik etki gözlenmedi. 15 mg/kg ACE uygulanan grupta kromozomal aberasyon oranının yüksekolduğu (P<0.001) belirlendi. Ayrıca 15 mg/kg ACE uygulanan grupta mitotik indeks oranın kontrol grubuna göre düşük olduğusaptandı. MN frekansları incelendiğinde, 5 mg/kg ve 10 mg/kg ACE uygulanan gruplar ile kontrol grubu arasında anlamlı birfark bulunamadı. Ancak kontrol grubuna göre 15 mg/kg asetamiprid uygulanan grupta, Mikronükleuslu polikromatik eritrositoranlarında istatistiksel olarak anlamlı bir artış gözlendi (P<0.001). ACE 15 mg/kg dozlarının genotoksik-sitotoksik etkili olduğusonucuna varıldı.

In this study, we aimed to determine acetamipridine’s (ACE) genotoxic effect in mus musculus var. albinos bonemarrow cells by chromosomal aberration (CA) and micronucleus (MN) analysis. Mice were divided into four groups. Group 1determined as negative control (NC), group 2, 3 and 4 were determined as 5 mg/kg ACE, 10 mg/kg ACE, and 15 mg/kg ACEgroups respectively. ACE was given orally for 14 days. CA, mitotic index (MI) and MN frequencies were determined in femoralcells. As a result, no genotoxic effects were observed in groups treated with 5-10 mg/kg ACE compared to negative control.CA rates of 15 mg/kg ACE group was determined to be high (P<0.001). In addition, MI ratio in the 15 mg/kg ACE group waslower than the control group. When the ratios of the polychromatic erythrocyte with micronucleus were examined, nosignificant difference was found between the 5 mg/kg and 10 mg/kg ACE groups and control group. However, a statisticallysignificant increase in MNPCE rates was observed in the 15 mg/kg ACE group compared to control ones (P<0.001). It wasconcluded that ACE at 15 mg/kg dose was genotoxic-cytotoxic.

Genotoxic Micronucleus Mitotic index Mus musculus albino

Mikronükleus Genotoksik Mitotik index Mus musculus albino

KAFKAS ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNATÖRLÜĞÜ

2017-FM-30

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