Ovaryohisterektomi Operasyonu Yapılan Ratlarda Kitosan'ın Postoperatif Peritoneal Adezyon Üzerine Etkisi

Abstract

Sunulan çalışmada ovaryohisterektomi operasyonundan hemen sonra intraperitonal yolla kitosan kullanımının postoperatif dönemde peritonal adezyon oluşumunun engellemesi üzerine olan etkisini araştırmak amaçlanmıştır. Araştırmada 250-350 gr ağırlığında toplam 15 adet dişi Wistar-albino rat kullanıldı. Ratlar operasyon öncesinde ve sonrasında oda ısısında (22±2°C), neminde, 12 saat ışık-12 saat karanlık ortamda barındırıldı. Tekli kafeslerde barındırılan ratlar için laboratuvar hayvanlarındaki standart bakım ve besleme yapıldı. Bu ratlar uygun anestezik yöntemler ile anesteziye alındıktan sonra, ovaryumlar ve uterus standart prosedurlere uygun olarak alındı. Ratlar daha sonra rastgele 2 gruba ayrıldı. Kitosan grubundaki ratlara (n=8) ovaryohisterektomi operasyonundan hemen sonra 50 mg/kg dozda kitosan 2.5 mL serum fizyolojik ile karıştırılarak bir defaya mahsus olmak üzere intraperitonal yolla verildi. Kontrol grubuna (n=7) ise ilk gruptan farklı olarak yalnızca 2.5 mL serum fizyolojik intraperitonal olarak verildi. Operasyon günü, operasyonu takip eden 7 ve 14. günlerde vücut sıcaklıkları kaydedildi. Hayvanlar operasyondan 21 gün sonra yüksek dozda anestezik verilerek ötenazi edildi. Vücut sıcaklıkları bakımından gruplar arasında fark saptanmadı. Adezyon belirlenmeyen rat oranının kitosan grubunda daha yüksek olduğu saptandı. Ayrıca kitosan grubunda adezyon direncinin daha düşük olduğu saptandı. Özellikle adezyon skoru 3 oranının kontrol grubunda daha yüksek olduğu saptandı. Bu sonuçlar intraperitoneal kitosan uygulamasının adezyon görülme oranını ve adezyon direncini düşürdüğünü göstermektedir.

Keywords

• Adezyon
• kitosan
• ovaryohisterektomi operasyonu
• Rat

Influence of Chitosan on the Postoperative Peritoneal Adhesion in Rats Undergoing Ovariohysterectomy

Abstract

In the present study, it is aimed to investigate the effect of intraperitoneal use of chitosan on the prevention of peritoneal adhesion formation in the postoperative period immediately after ovariohysterectomy. A total of 15 female Wistar-albino rats weighing 250-350 gr were used for this researcher. Rats were housed in room temperature (22±2°C) and humidity, 12h light-dark cycles. The rats housed in single cages were fed with standard laboratory food and water. After the rats were anesthetized with appropriate anesthetic methods, ovarium and uterus were removed according to the procedure standard procedures used in routines. Rats were randomly divided into 2 groups. Chitosan groups (n=8) were injected intraperitoneally as a single dose mixing 2.5 mL of physiological saline solution with a dose of 50 mg/kg of chitosan immediately after OVH operation. Rats in the other group (n=7), which were evaluated as the control group, were given only 2.5 mL of saline intraperitoneally different from the first group. Body temperatures were recorded on the day of the operation (day 0) and on the 7th and 14th day following the operation. The animals were euthanized 21 days after the operation with a high dose of anesthetic. There was no difference between the groups in terms of body temperatures. Rat without adhesion was found to be higher in the chitosan group. In addition, adhesion resistance was found to be lower in chitosan group. Especially, the number of adhesions score 3 was higher in the control group. These results show that intraperitoneal chitosan application decreases the adhesion rate and adhesion resistance.

Keywords

• Adhesion
• Chitosan
• Ovariohystorectomy operaiton
• Rat

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