Akış Sitometrisinin (Flow Cytometry) Sperma Kalite Analizlerinde Kullanımı

Year 2020, Volume: 15 Issue: 1, 76 - 83, 30.04.2020

Fırat Korkmaz , Beste Çil

https://doi.org/10.17094/ataunivbd.614120

Abstract

Spermanın kalite analizleri, infertilitenin tanımlanmasında ve yardımcı üreme teknikleri yönünden günümüz bilim dünyasında değer kazanmaya başlamıştır. Bu analizlerde tercih edilen yöntemlerin en gelişmişlerinden biri olan akış sitometrisi, akış halindeki bir süspansiyon içindeki çeşitli boyut ve yapıdaki hücre, nükleus ve benzeri hücre komponentleri, kromozomal yapılar ve çeşitli partikülleri de kapsayan taneciklerin, lazer ve özelleştirilmiş dedektörler yardımıyla nitelik ve nicelik özelliklerinin ölçülmesine dayanan bir yöntemdir. Bu amaçla, florokrom moleküllerle boyanmış örneklere ait partiküllerin tek sıralı hale getirilmesinin ardından optik sistemden saçılan lazer ışınına maruz kalmakta, geri dönen ışınlar ise toplanarak elektronik sinyallere dönüştürülmektedir. Elektronik sinyaller ise yazılımlar ile değerlendirilmektedir. Akış sitometrisinin, geniş kullanım yelpazesi içerisinden, gelişen infertilite teşhis metodları ve yardımcı üreme teknikleri sayesinde ilk olarak beşeri androloji laboratuvarlarında, hemen ardından ise veteriner androloji laboratuvarlarında, spermatozoanın konsantrasyonu; plazma membran ve akrozom bütünlüğü; apoptosis; mitokondriyal membran potansiyeli; kapasitasyon; oksidatif stres; lipid peroksidasyonu; DNA bütünlüğü ve cinsiyetin belirlenmesi vb. amaçlar ile kullanılmaya başlanmıştır. Bu derlemede akış sitometrisi ve analizlerinin sperma kalitesini belirlemedeki rolü ve önemi açıklanarak ilgili sperm kalite parametrelerinin ölçümünde kullanılan protokoller hakkında detaylı bilgilendirme sağlanması hedeflenmiştir. 

Keywords

Sperma Analizi, Suni Tohumlama, Veteriner Androloji, : Akış Sitometrisi, Cinsiyeti Belirlenmiş Sperma, Sperma Analizi, Suni Tohumlama, Veteriner Androloji

The Use of Flow Cytometry in Semen Quality Analyses

Abstract

Quality analysis of semen has started to gain value in the recent scientific world in terms of defining infertility and assisted reproductive techniques. Flow cytometry, the most advanced method of choice in these analyzes, is a method based on measuring the quality and quantity of particles including cells, nucleus, chromosomal structures and various particles with its sizes and structures in a flowing suspension by means of lasers and detectors. For this purpose, the particles that are stained with fluorochrome molecules are exposed to the laser beam scattered from the optical system after being unified, and the returning rays are collected and converted into electronic signals. Electronic signals are evaluated with softwares. Within a wide range of uses, i.e. the concentration of spermatozoa; plasma membrane and acrosome integrity; apoptosis; mitochondrial membrane potential, capacitation status; oxidative stress; lipid peroxidation; DNA integrity and gender determination purposes etc., it has been used as infertility diagnostic methods and assisted reproductive techniques which have been developed, firstly in human andrology laboratories and then in veterinary andrology laboratories. The aim of this review is to explain the role and importance of flow cytometry and in determining sperm quality and to provide detailed information about the relevant protocols.

Keywords

Artificial insemination, Semen Analyses, Sexed Semen, Veterinary Andrology, Flow cytometry

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