Wistar Ratlarında Dietilnitrozamin Uygulanarak Oluşturulan Hepatoselüler Karsinom Modelinde Silimarin’in Koruyucu Etkisi; Kanser Paternleri ve Sitolojik Varyantlar

Year 2020, Volume: 15 Issue: 1, 43 - 50, 30.04.2020

Emin Karakurt Serpil Dağ

https://doi.org/10.17094/ataunivbd.680945

Cited By: 3

Abstract

Bu çalışmada, ratlarda deneysel olarak Dietilnitrozamin uygulanarak oluşturulan Hepatoselüler karsinom modelinde Silimarin’in koruyucu etkisi kanser paternleri ve sitolojik varyantlar açısından histopatolojik metodlarla araştırılmıştır. Bu amaçla ratlar 5 gruba ayrıldı. Kontrol grubundaki ratlara 20 hafta boyunca pelet yem ve içme suyu verildi. Sham grubundaki hayvanlara %0.9 NaCl içerisinde çözdürülmüş propilen glikol intraperitoneal yolla haftada 3 kez olmak üzere 20 hafta boyunca verildi. DEN grubundaki hayvanlara 50 mg/kg dozunda haftada 1 kez olmak üzere 20 hafta boyunca DEN uygulaması yapıldı. DEN+Silimarin grubundaki ratlara DEN uygulamasından 1 hafta önce başlayacak şekilde 100 mg/kg dozunda haftada 3 kez olmak üzere 21 hafta boyunca intraperitoneal yolla Silimarin uygulandı. Silimarin grubundaki ratlara 100 mg/kg dozunda 20 hafta boyunca intraperitoneal yolla haftada 3 kez olmak üzere Silimarin uygulandı. Çalışmanın sonunda, hayvanların sistemik nekropsisi yapıldı ve karaciğer doku örnekleri alındı. Rutin doku takip işlemlerinden sonra kesitler ışık mikroskobu altında değerlendirildi. DEN ve DEN+Silimarin gruplarında Hepatoselüler karsinom oluştuğunu gözlemledik

Keywords

Dietilnitrozamin, Hepatoselüler Karsinom, Paternler, Silimarin, Varyantlar

Supporting Institution

KAÜ Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

2016-TS-08

Protective Effect of Silymarin on Diethylnitrosamine Induced Hepatocellular Carcinoma Model in Wistar Rats; Cancer Patterns and Cytological Variants

Abstract

In this study, the protective effect of silymarin was investigated with histopathological methods in terms of cancer patterns and cytological variants in the hepatocellular carcinoma model created by applying diethylnitrosamine experimentally in rats. For this purpose, the rats were randomly divided into 5 groups. The rats in the control group were fed pellet feed and drinking water for 20 weeks. Animals in Sham group were administered with propylene glycol dissolved in 0.9% NaCl intraperitoneally 3 times a week for 20 weeks. Animals in DEN group administered DEN at a dose of 50 mg/kg once a week for 20 weeks. The rats in the DEN +Silymarin group were Silymarin intraperitoneally administered for 21 weeks 3 times a week at a dose of 100 mg/kg, starting 1 week prior to the administration of DEN. Silymarin was administered to rats in the group of Silymarin 3 times a week intraperitoneally for 20 weeks at a dose of 100 mg/kg. At the end of the study, systemic necropsy of the animals was performed and liver tissue samples were taken. After routine tissue procedures, sections were examined under the light microscope. We observed that Hepatocellular carcinoma occurred in DEN and DEN + Silymarin groups.

Keywords

Diethylnitrosamine, Hepatocellular Carcinoma, Patterns, Silymarin, Variants

Project Number

2016-TS-08

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