Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti

Year 2019, Volume: 2 Issue: 1, 17 - 23, 31.01.2019

Özer Akgül Hayriye Kırkoyun Uysal İlker Tosun Burcu Sapmaz Reyhan Çalışkan A. Canan Yazıcı Güvercin Sinem Öktem Özgür Kurt Y. Ali Öner

Abstract

Objective: Intestinal parasitic infections are among the prominent public health concerns in
patients with suppressed immune function.
Material and methods: Here we examined stool specimens by microscopy and polymerase
chain reaction (PCR) to evaluate the infection of five principal protozoans (Cryptosporidium
spp., Giardia spp., Entamoeba histolytica, Blastocystis spp. and Dientamoeba spp.) among 90
renal transplant recipient patients (RTP) in comparison with 90 healthy individuals (HI) from
Turkey.
Results: The overall frequency of any parasites was 17.2% (31/180) with microscopy and
51.7% (93/180) with PCR. Because of its high sensitivity, PCR was compared with microscopy
in terms of the accuracy of detecting intestinal parasites, and the agreement was found to be
inadequate (κ= 0.217; p<0.001). Multiparasitism (90.9%), Cryptosporidium spp. (84.6%) and
Giardia spp. (74.1%) were the most frequent agents in RTP, respectively (p<0.001).
Conclusions: This is the first study performed in Turkish reporting the prevalence of five
intestinal parasites with PCR techniques among this group and seeks to provide a basis for
future studies  

Keywords

Intestinal parasites, Transplantation, PCR, Microscopy

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