Ankara Üniversitesi Tıp Fakültesi Mecmuası 0365-8104 1307-5608 Ankara University Gastroenterology and Hepatology Gastroenteroloji ve Hepatoloji Çeşitli Hastalıklarda Dinitroklorobenzen (NDCB) Testi Dinitrochlorobenzene (NDCB) Testing in Various Diseases Alptuna Erdem ANKARA ÜNİVERSİTESİ, ANKARA TIP FAKÜLTESİ 12 30 1974 27 3-4 585 598 09 01 1974 12 30 1974 Copyright © 1947, Ankara Üniversitesi Tıp Fakültesi Mecmuası 1947 Ankara Üniversitesi Tıp Fakültesi Mecmuası

Kronik böbrek hastalığı, kronik karaciğer hastalığı, metastatik kanser, akut lökoz ve malin lenfomalarda gecikmiş tipde hipersensitivitenin (GTH) ve dolayısıyla T - hücre fonksionlarının durumunu tesbit için toplam 36 hastaya 2,4, dinitrochloro - benzene (DNCB) testi tatbik edildi. Test neticelerinin analizisonucu kronik böbrek hastalarında GTH reaksionları ile NPN arasında yakın bir ilişki tesbit edildi Yüksek seviyelere ulaşan NPN'nin T- hücre fonksionlarını inhibe ettiği ve bu durumun NPN'in normalleşmesi ile reversible olduğu saptandı, Akut lökoz ve malin lenfomalarda DNCB ile elde edilen neticeler dünya literatürüne uygunluk arzetmektedir. Kronik karaciğer hastalarında GTH'nin normallere nazaran hafif azalmış olduğu anlaşıldı.

A total of 36 patients were administered the 2.4-dinitrochlorobenzene (DNCB) test to determine delayed-type hypersensitivity (TTH) and, consequently, T-cell function in patients with chronic kidney disease, chronic liver disease, metastatic cancer, acute leukosis, and malignant lymphoma. Analysis of the test results revealed a close relationship between TTH reactions and NPN in patients with chronic kidney disease. It was determined that high levels of NPN inhibited T-cell function, and that this was reversible with normalization of NPN. The results obtained with DNCB in acute leukosis and malignant lymphoma are consistent with international literature. It was observed that TTH in patients with chronic liver disease was slightly decreased compared to normal levels.

 Kidney T Cells function Böbrek T Hücreleri Fonksiyon Ankara Üniversitesi Tıp Fakültesi Gastroenteroloji Kliniği Kürsüsü 1 — Rogoch, A., Gastroenterology, McGraw-Hill Book Company, Blakiston Pub. New York. s. 739, 1973. 2 — Sherlock, S., Diseases of the Liver and Biliary System, Blackwell Scientific Pub., Oxford and Edinburgh, 4th Edward Printing, s. 430, 1971. 3 — Starauss, M. B., Welt, L. G., Diseases of the Kidney, Little Brown and Comp. Boston, 2nd Ed. s. 739-405, 1971. 4 — Willams, W. J., Beutler, E., Erslev, A. J., Rundles, R. W., Haematology, McGraw-Hill Book Comp. New York, Chap. 157 ve 160, 1972. 5 — Gold, P., Freedman, S., Demonstration of tumor specific antigens in human colonic carcinomata by immunologic tolerance and adsorption techniques, J. Exp. Med. 121:439, 1965. 6 — Hakkinen, I., An immunochemical method for detecting carcinomatous secretion from gastric juice, Scand. J. Gastro. 1:28, 1966. 7 — Uriel, J., de Nechaud, B., Stanislawski, M., Birengwajg, M., Le diagnostic du cancer primaire de foie par des méthodes immunologiques, Presse. Med. 76:1415, 1968. 8 — Waksman, B. H., Measurement of cell mediated-immunity, New Eng. J. Med. 268:431, 1972. 9 — Catalona, W. J., Taylor, P. T., Rabson, A. S., Chretien, P. B.: A method for DNCB contact sensitisation: A clinicopathological study, New Engl. J. Med. 286:399, 1972. 10 — Eisen, H. N., Belman, S., Carsten, M. E.: The reaction of 2,4-dinitrochlorobenzene sulfonic acid with free amino groups of proteins, J. Am. Chem. Soc. 75:4583, 1953. 11 — Eisen, H. N., Tabachnic, M.: Elicitation of allergic contact dermatitis in the guinea pig: The distribution of bound dinitrobenzenes groups within the skin and quantitative determination of the extent of combination of 2,4-dinitrochlorobenzene with epidermal protein in vivo, J. Exp. Med. 108:773, 1958. 12 — Eisen, H. N., Orris, L., Belman, S.: Elicitation of delayed allergic skin reactions with haptens: The dependence of elicitation on hapten combination with protein, J. Exp. Med. 95:473, 1952. 13 — Sadoff, L., Giovsky, M., Alenty, A.: DNCB test in cancer patient, New Engl. J. Med. 287:47, 1972. 14 — Hunt, J. S., Silverstein, M. J., Sparkes, F. C., Haskell, G. C. M.: Granulomatous hepatitis: A complication of BCG immunotherapy, New Engl. J. Med. 286:399, 1972. 15 — Sadoff, L.: The usefulness of medroxyprogesterone acetate in treatment of advanced cancer, baskıda, mektup ile, 1973. 16 — Brown, R. S., Haynes, H. A., Folley, H. T., Gedwin, H. A., Berard, C. W., Carbone, P. P.: Hodgkin's: Immunologic, clinical and histologic features of 50 untreated patients, Ann. Int. Med. 67:291, 1967. 17 — Aisenberg, A. C.: Lymphocytopenia in Hodgkin's disease, Blood 25:1037, 1965. 18 — Zamcheck, M., Moore, T. L., Dhar, P., Kupchik, H.: Current concepts: Immunologic evaluation of human digestive tract cancer. Carcinoembryonic antigens, New Engl. J. Med. 286:83, 1972. 19 — Whitehouse, J. M. A., Holborow, E. J.: Smooth muscle antibody in malignant disease, Brit. Med. J. 4:511, 1971. 20 — Sokal, J. E., Primikirios, E.: The delayed skin test response in Hodgkin's disease and lymphosarcoma, Cancer 14:597, 1961. 21 — Barr, M., Fairley, G. H.: Circulating antibodies in reticulosis, Lancet I:1901, 1963. 22 — Leskowitz, S., Philippino, L., Hendrik, C., Graham, J. B.: Immune response with patients with cancer, Cancer 10:1103, 1957. 23 — Aisenberg, A. C.: Studies on Delayed Hypersensitivity in Hodgkin's Disease, J. Clin. Invest. 41:1964, 1962. 24 — Dubin, I. N.: The poverty of the immunological mechanisms in patients with Hodgkin's Disease, Ann. Int. Med. 27:898, 1947. 25 — Longmire, R. L., MacMillan, R., Yelenovsky, R., Armstrong, S.: In vitro splenic Ig synthesis in Hodgkin's Disease, New Engl. J. Med. 289:763, 1973. 26 — Schier, W., Roth, A., Ostroft, G., Schrift, M. H.: Hodgkin's disease and Immunity, Amer. J. Med. 20:94, 1956. 27 — Wisemann, L.: The blood pictures in the primary diseases of the lymphoid system: Their character and significance, JAMA 107:2016, 1936. 28 — Aisenberg, A. C.: Studies on lymphocyte transfer reactions in Hodgkin's disease, J. Clin. Invest. 44:555, 1965. 29 — Smiddy, F. G., Burnell, R. G., Parsons, F. M.: Influence of uremia on the survival of skin homografts, Nature 190:732, 1961. 30 — Lamb, D., Pilney, F., Kelly, W. D., Good, R. A.: A comparative study of the incidence of anergy in patients with carcinoma, leukemia, Hodgkin's disease and other lymphomas, J. Immunol. 89:555, 1962. 31 — Aisenberg, A. C.: Immunologic status of Hodgkin's Disease, Cancer 19:385, 1966. 32 — Peckham, M. J., Cooper, B. M. H.: Proliferation characteristics of the various classes of cells in Hodgkin's disease, Cancer 24:135, 1969. 33 — Walker, G., Doniach, D.: Antibodies and Immunoglobulins in Liver Disease, Gut 9:266, 1968. 34 — Blumberg, B. S., Alter, H. J., Visnich, S.: A new antigen in leukemia sera, JAMA 1191:6541, 1965. 35 — Alptuna, R., Türkyan, M., Koca, Y., Uzunalimoğlu, Ö.: Kontrimmune-elektroforez metodu ile Avustralya (Au/SH) antijeni araştırılmasında yeni bir tampon solüsyonu denenmesi, A. Ü. Tıp Fak. Mec. 25:603, 1972. 36 — Bogoch, A.: Gastroenterology, McGraw-Hill Book Comp. New York, s. 1312, 1973. 37 — Poronetto, F., Schaffner, F., Popper, H.: Immunocytochemical and serologic observations in primary biliary cirrhosis, New Engl. J. Med. 271:1123, 1964. 38 — Doniach, D., Roitt, I. M., Walker, J. G., Sherlock, S.: Tissue antibodies in primary biliary cirrhosis, active chronic hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implication, Clin. Exp. Immunol. 1:237, 1966. 39 — Lange, K., Wasserman, E., Slobody, L. B.: The significance of serum complement levels with the diagnosis and prognosis of acute and subacute glomerulonephritis. 40 — Wilson, I. D., Williams, R. C., Tobian, L.: Renal tubular acidosis: Three cases with immunoglobulin abnormalities in the patients and their kindred, Amer. J. Med. 43:356, 1967. 41 — Lospalluto, J., Doward, B., Miller, W., Zift, M.: Cryoglobulinemia based on interaction between a macroglobulin and 7S gamma globulin, Amer. J. Med. 32:142, 1962. 42 — Strauss, M. B., Welt, L. G.: Diseases of the Kidney, Little Brown and Comp. Boston. 2nd Ed. s. 1430, 1971. 43 — Mills, J. A.: The immunologic significance of antigen induced lymphocyte transformation in vitro, J. Immunology 97:239, 1966. 44 — Milgram, F., Litvak, B. I., Kand, K.: Humoral antibodies in renal homograft, JAMA 198:226, 1956. 45 — 1971 Year Book of Medicine, Year Book Medical Publications, s. 609, 1971. 46 — Aisenberg, A. C., Leskowitz, S.: Antibody formation in Hodgkin's disease, New Engl. J. Med. 268:1269, 1963.