ankara univ vet fak derg Ankara Üniversitesi Veteriner Fakültesi Dergisi 1300-0861 1308-2817 Ankara University 10.1501/Vetfak_0000002835 Veterinary Surgery Veteriner Cerrahi Determination of optimal conditions for activity of arginase in kidney of rabbit Tavşan böbrek arginaz aktivitesi için optimal şartların belirlenmesi Şengül Beytullah Erişir Mine 06 01 2018 65 2 0 2 06 01 2018 Copyright © 1954, Ankara Üniversitesi Veteriner Fakültesi Dergisi 1954 Ankara Üniversitesi Veteriner Fakültesi Dergisi

Optimum conditions for arginase in kidney tissue may differ from those for arginase of liver. Because optimum conditions for measuring arginase activity in rabbit kidney tissue were not determined, it was aimed to optimize arginase of rabbit kidney tissue and compare it with arginase of liver. In the present study, the kidneys of six New Zealand rabbits aged 1 year old were used. It was found to be preincubation temperature 53 °C, preincubation period is 15 minutes, incubation period is 10 minutes and optimum pH is 10.1 in NaHCO3-NaOH buffer for arginase in rabbit kidney tissue. Enzyme achieved its highest activity at 1 mM MnCl2concentration. Km of arginase in rabbit kidney tissue for L-arginine was measured as 12.5 mM. It was determined that Mn+2 ions and preincubation at 53 °C were essential for the activation of the enzyme. As a result, optimal conditions for the kidney arginase were found to be different from those for the liver arginase. Our study indicates that arginases encoded by separate gene loci may have different optimal conditions

Böbrek dokusundaki arginazın optimum şartları karaciğer arginazınınkinden farklı olabilir. Tavşan böbrek dokusundaki arginazın ölçülmesi için optimum şartlar tespit edilmediğinden böbrek dokusundaki arginazın optimize edilmesi ve karaciğer arginazınınki ile karşılaştırılması amaçlanmıştır. Çalışmada bir yaşında 6 adet Beyaz Yeni Zelanda Tavşanı’nın böbrek dokuları kullanıldı. Tavşan böbrek doku arginazı için preinkübasyon ısısı 53°C, preinkübasyon zamanı 15 dakika, inkübasyon zamanı 10 dakika, en uygun tampon NaHCO3-NaOH tamponu ve optimum pH 10.1 şeklinde tespit edilmiştir. Enzimin aktivitesi, 1 mM MnCl2 konsantrasyonunda en yüksek değere ulaşmıştır. Tavşan böbrek dokusu arginazının L-arginine karşı olan Km’i 12.5 mM olarak bulunmuştur. Enzimin aktive olması için Mn+2 iyonları ve 53 °C’ de preinkübasyon gereklidir. Böbrek arginazı için tayin edilen optimum şartlar karaciğer arginazınınkinden farklı olarak bulundu. Yapılan çalışma, ayrı gen lokusları tarafından kodlanan arginazların farklı optimum şatlara sahip olduğunu göstermektedir

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