ankara univ vet fak derg Ankara Üniversitesi Veteriner Fakültesi Dergisi 1300-0861 1308-2817 Ankara University 10.1501/Vetfak_0000002828 Veterinary Surgery Veteriner Cerrahi Solunum sistemi problemli taylarda equid alpha ve gammaherpesvirus enfeksiyonlarının varlığınınbelirlenmesi Determination of presence of equid alpha and gammaherpesvirus infections in foals with respiratory distress Dağalp Seval Bilge Babaoğlu Ali Rıza Ataseven Veysel Soydal Karapınar Zeynep Timurkan Mehmet Özkan Doğan Fırat Özkul Aykut Alkan Feray 03 01 2018 65 1 63 68 03 01 2018 Copyright © 1954, Ankara Üniversitesi Veteriner Fakültesi Dergisi 1954 Ankara Üniversitesi Veteriner Fakültesi Dergisi

Bu çalışmanın amacı, özel bir pansiyon harasında (geçici konaklama yeri) bulunan taylarda bir solunum sistemi hastalığı salgınında equid herpesvirusların varlığını araştırmak ve equid alpha ve gammaherpesvirusların olası etkileşimlerini ortaya koymaktır. Bu amaçla, solunum sistemi hastalığı bulgulu 28 taydan nazal swap (n=21), lökosit örnekleri (n=28) ve ölen bir taydan alınan doku örnekleri, equid herpesvirus-1, -4, -2 ve -5 yönünden glikoprotein B (gB) genini tespit etmeyi hedefleyen multipleks nested PCR ile test edildi. Örneklenen 29 hayvanın %3.4’ü (1/29), %58.6’sı (17/29), %58.6’sı (17/29) ve %75.9’u (22/29) sırasıyla EHV-1, EHV-4, EHV-2 ve EHV-5 yönünden pozitif olarak bulundu. Özellikle söz konusu viruslar tarafından oluşturulan tekli enfeksiyonlardan çok, EHV-1,-2 ve -5 arasında çoklu enfeksiyonların varlığı (%75.9; 22/29) dikkat çekiciydi. Filogenetik analiz sonuçları yerel equid gammaherpesvirusların, EHV-4 suşlarının aksine genetik olarak büyük farklılık gösterdiğini ortaya koydu

The aim of this study was to investigate the presence of equid herpesviruses and to elucidate the possible mutual effects of equid alpha and gammaherpesviruses in an outbreak of respiratory tract disease in foals in a private pension stable (transient residency stable), Turkey. For this purpose, nasal swabs (n=21) and peripheral blood leukocytes (n=28) from 28 foals with respiratory tract diseases, and tissue samples from one dead foal were tested for equid herpesvirus-1, -4, -2 and -5 by multiplex nested PCR targeting the glycoprotein B (gB) gene. Of the 29 sampled animals, 3.4% (1/29), 58.6% (17/29), 58.6% (17/29) and 75.9% (22/29) were found positive for EHV-1, EHV-4, EHV-2 and EHV-5, respectively. Especially, a high ratio of multiple infections (75.9%; 22/29) caused by EHV-4, EHV-2 and EHV-5 were detected in the tested foals. The phylogenetic analysis showed that our equid gammaherpesviruses had a high degree of genetic heterogeneity, in contrast to our EHV-4 strains analyzed

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