ankara univ vet fak derg

Ankara Üniversitesi Veteriner Fakültesi Dergisi

1300-0861 1308-2817

Ankara University

10.1501/Vetfak_0000002810

Veterinary Surgery

Veteriner Cerrahi

Isolation of Listeria monocytogenes in lamb meat and determination of the antibiotic resistance

Kuzu etlerinden Listeria monocytogenes izolasyonu ve antibiyotik dirençliliklerinin belirlenmesi

Kocaman

Nurcay

The Ministry of Health, Public Health Institution of Turkey; Sarımehmetoğlu

Belgin

The Ministry of Health, Public Health Institution of Turkey;

12 01 2017

64 4 273 279 12 01 2017

1954

Ankara Üniversitesi Veteriner Fakültesi Dergisi

In this study the occurrence of Listeria monocytogenes and other Listeria species were investigated from a total of 120 retail raw lamb meat samples and L. monocytogenes isolates were verified by using PCR based on hylA gene which is responsible for virulence. All L. monocytogenes isolates were analyzed by antimicrobial susceptibility tests. L. monocytogenes was isolated from 19 (15.8%) of the samples examined. The other species isolated were L. innocua, L. weshimeri and L. grayi 26 (21.6%), nine (7.5%) 2 (1.6%) strains respectively. According to the PCR analysis, hlyA gene was detected from the 58 of the isolates which were isolated from L. monocytogenes positive 19 lamb meat samples. The antibiotic resistance profiles of the 58 isolates to nine antibiotics were detected by disc diffusion method. Five (8.6%) isolates were found to be resistant to antibiotics. Among the five isolates, four (6.9%) displayed multiple resistance, only one isolate was resistant to tetracycline

Bu çalışmada, satışa sunulan toplam 120 çiğ kuzu eti örneklerinde Listeria monocytogenes ve diğer Listeria türlerinin varlığı araştırılmış ve bulunan L. monocytogenes izolatları virülensten sorumlu hylA geni esas alınarak PZR ile doğrulanmıştır. Bütün L. monocytogenes izolatları antibiyotik duyarlılık testi ile analiz edilmiştir. Test edilen örneklerin 19’undan (15.8%) L. monocytogenes izole edilmiştir. İzole edilen diğer türler sırasıyla 26 adet (21.6%) L. innocua, dokuz adet (7.5%) L. weshimeri ve iki adet (1.6%) L. grayi’dir. PZR analizine göre 19 kuzu eti örneğinden izole edilen L. monocytogenes pozitif izolatların 58’inde hlyA geni belirlenmiştir. 58 izolatın dokuz antibiyotiğe karşı antibiyotik direnç profili disk difüzyon metodu kullanılarak belirlenmiştir. Beş izolatın (8.6%) antibiyotiklere karşı dirençli olduğu bulunmuştur. Bu beş izolat arasında dördü (6.9%) çoklu direnç gösterirken, bir izolat sadece tetrasikline karşı dirençli bulunmuştur

Antimicrobial resistance food safety lamb meat Listeria PCR

Antimikrobiyal direnç gıda güvenliği kuzu eti Listeria PZR

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