ankara univ vet fak derg

Ankara Üniversitesi Veteriner Fakültesi Dergisi

1300-0861 1308-2817

Ankara University

10.1501/Vetfak_0000002813

Veterinary Surgery

Veteriner Cerrahi

Characterization of extended-spectrum beta-lactamase-producing fecal Escherichia coli isolates in laying hens

Yumurtacı tavuklarda genişlemiş spektrumlu beta laktamaz üreten dışkı kökenli Escherichia coliizolatlarının karakterizasyonu

Pehlivanoğlu

Faruk

Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, Burdur; Türütoğlu

Hülya

Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, Burdur; Öztürk

Dilek

Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, Burdur; Yardımcı

Hakan

Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, Burdur;

12 01 2017

64 4 301 306 12 01 2017

1954

Ankara Üniversitesi Veteriner Fakültesi Dergisi

The purposes of the present study were to determine whether healthy laying hens in Burdur province of Turkey carried extended-spectrum beta-lactamase (ESBL)- producing Escherichia coli in their intestinal flora and to further characterize the E. coli isolates and ESBL genes. A total of 200 fecal samples from three laying hen farms were cultured in Brilliance E. coli / coliform Selective Agar supplemented with cefotaxime or ceftazidime. ESBL-producing E. coli isolates determined by phenotypic tests were screened by Polymerase Chain Reaction (PCR) for ESBL genes, and ESBL genes were sequenced. Phylogenetic analysis of the E. coli isolates were performed by PCR. ESBL-producing E. coli were isolated from 12 (6.0%) laying hen fecal samples by phenotypic tests. According to PCR screening and sequence analysis, blaCTX-M group 1 (blaCTX-M-3 or blaCTX-M-15) genes were detected in the E. coli isolates. Phylogenetic analysis of the E. coli isolates showed that group D was the predominant group (58.3%) and none of the isolates belonged to group B2. Consequently, present study reports the first characterisation of CTX-M-3 and CTX-M-15 type ESBL-producing E. coli isolates on laying hen production from Turkey

Bu çalışmada, Burdur ilindeki yumurtacı tavukların bağırsak mikroflorasında genişlemiş spektrumlu beta laktamaz (GSBL) üreten Escherichia coli varlığının ortaya çıkarılması ve izole edilen E. coli izolatlarının ve taşıdıkları GSBL genlerinin karakterize edilmesi amaçlandı. Toplam üç tavuk çiftliğinden alınan 200 dışkı örneği, içerisine sefotaksim ve seftazidim ilave edilmiş Brilliance E. coli / koliform Selektif Agara ekildi. Fenotipik testlerle belirlenen GSBL üreten E. coli izolatları GSBL genleri yönünden Polimeraz Zincir Reaksiyonu (PZR) ile tarandı ve GSBL genlerinin sekans analizi yapıldı. İzolatların filogenetik analizi PZR ile gerçekleştirildi. Fenotipik testler ile GSBL üreten E. coli 12 (%6.0) adet dışkı örneğinde belirlendi. PZR ve sekans analizine göre, E. coli izolatlarında blaCTX-M grup 1 genleri (blaCTX-M-3 ve blaCTX-M-15) belirlendi. İzolatların filogenetik analizi grup D’nin en yaygın olduğunu (%58.3) ve izolatların hiç birinin B2 grubundan olmadığını gösterdi. Sonuç olarak, bu çalışma Türkiye’de yumurta tavuklarında CTX-M-3 and CTX-M-15 type GSBL üreten E. coli izolatlarını tanımlayan ilk rapordur

CTX-M-3 CTX-M-15 Escherichia coli laying hens phylogenetic analysis

CTX-M-3 CTX-M-15 Escherichia coli filogenetik analiz yumurtacı tavuk

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