ankara univ vet fak derg Ankara Üniversitesi Veteriner Fakültesi Dergisi 1300-0861 1308-2817 Ankara University 10.1501/Vetfak_0000002816 Veterinary Surgery Veteriner Cerrahi Serotyping, genetic characterization and antimicrobial susceptibility determination ofVibrio anguillarum strains isolated from farmed rainbow trout (Oncorhynchus mykiss) in the easternBlack Sea Doğu Karadeniz’de yetiştiriciliği yapılan gökkuşağı alabalıkları(Oncorhynchus mykiss)’ndan izole edilen Vibrio anguillarum suşlarınınserotiplendirilmesi, genetik karakterizasyonu ve antimikrobiyalduyarlılığının belirlenmesi Balta Fikri Recep Tayyip Erdoğan Üniversitesi, Su Ürünleri Fakültesi, Hastalıklar Anabilim Dalı, Rize, Türkiye. Dengiz Zeynep Balta Recep Tayyip Erdoğan Üniversitesi, Su Ürünleri Fakültesi, Hastalıklar Anabilim Dalı, Rize, Türkiye. 12 01 2017 64 4 321 328 12 01 2017 Copyright © 1954, Ankara Üniversitesi Veteriner Fakültesi Dergisi 1954 Ankara Üniversitesi Veteriner Fakültesi Dergisi

Antibiotic resistance profile, serotyping and identification of Vibrio anguillarum isolated from rainbow trout (Oncorhynchus mykiss) cultured in cages in the Eastern Black Sea Region of Turkey, were performed in the study. The biochemical, serological and molecular techniques were used in identification of V. anguillarum isolates. The sequenced isolates were confirmed to be similar to V. anguillarum which stored under different accession numbers in GenBank database. The rate of 97-99% similarity were found among the compared isolates. All isolates were identified to be serotype O1 by slide agglutination test results. The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol

Bu çalışmada, Türkiye’nin Doğu Karadeniz Bölgesi’nde kafeslerde yetiştirilen gökkuşağı alabalık (Oncorhynchus mykiss)’larından izole edilen Vibrio anguillarum suşlarının identifikasyonu ve serotiplendirilmesi yapıldı ve antimikrobiyal direnç profilleri belirlendi. V. anguillarum suşlarının tanımlanmasında biyokimyasal, serolojik ve moleküler teknikler kullanıldı. Dizi analizine gönderilen şuşlar GenBank veri tabanında farklı kabul numarasıyla kayıtlı olan V. anguillarum ile karşılaştırıldı. Karşılaştırma yapılan suşlar arasında %97-99 oranında benzerlik olduğu tespit edildi. Lam aglütinasyon test sonuçlarına göre, tüm izolatların serotip O1 olduğu belirlendi. Antimikrobiyal duyarlılık test sonuçlarına göre, izolatların sulphamethoxazole (%100), ampicilline (%90,6), eritromisine (%71,9), oksitetrasiklin (%62,5), streptomisin’e (% 46,9) ve trimetoprim+sulfametoksazol (%31,3) dirençli olduğu, fakat oksolinik asit, enrofloksasin ve florfenikole duyarlı olduğu tespit edildi

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