ankara univ vet fak derg

Ankara Üniversitesi Veteriner Fakültesi Dergisi

1300-0861 1308-2817

Ankara University

10.1501/Vetfak_0000002817

Veterinary Surgery

Veteriner Cerrahi

Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat flocks

Seçilen keçi sürülerinde gB/gE ELISA kombinasyonu ve VNT kullanılarak BoHV-1 ve CpHV-1enfeksiyonlarının seroprevalansının araştırılması

Özgür

Merve Baydin

Etlik Veterinary Control Central Research Institute Bilge

Seval Dağalp

Etlik Veterinary Control Central Research Institute

12 01 2017

64 4 329 335 12 01 2017

1954

Ankara Üniversitesi Veteriner Fakültesi Dergisi

In this study, we have investigated the seroprevalence of alphaherpesvirus (BoHV-1 and CpHV-1) infections in selected goat flocks using glycoprotein B (gB)/glycoprotein E (gE) ELISA combination and virus neutralisation test (VNT). For this purpose, we collected blood serum samples of 546 Saanen goats from Bolu province and tested them by ELISA and VNT. Using ELISA, 32.05% (175/546) of the samples were found to be gB(+)/gE(-) for CpHV-1, whereas only 0.73% (4/546) of the samples were noted to be gB(+)/gE(+) for BoHV-1 releated infection. By performing VNT, we found 31.86% (174/546) and 3.29% (18/546) positivity for CpHV-1 and BoHV-1, respectively. In conclusion, prevalence of CpHV-1 infection was found to be higher than BoHV1 infection in sampled goat flocks. For diagnosis of alphaherpesviruses in the goats, use of gB blocking ELISA test was found to be favorable as an alternative method. On the other hand, to distinguish between the CpHV-1/BoHV-1, gE blocking ELISA in combination by VNT was found to be incompatible with the statistical analysis (p<0.001). Considering antigenic cross-reactions between these viruses, because of the incompatibility of these tests, using a more sensitive/specific method to determine CpHV-1 and BoHV-1 antibodies can be suggested

Bu çalışmada, seçilmiş keçi sürülerinde BoHV-1 ve CpHV-1 enfeksiyonlarının seroprevalansı glikoprotein B (gB)/ glikoprotein E (gE) ELISA kombinasyonu ve virus nötralizasyon testi (VNT) kullanılarak araştırılmıştır. Bu amaçla, Bolu ilinden 546 Saanen keçisinden alınan kan serumu örnekleri ELISA ve VNT kullanılarak test edilmiştir. Her iki ELISA sistemi ile test edilen örneklerin %32.05’i (175/546) CpHV-1 antikor pozitifliğini yansıtan gB(+)/gE(-) olarak bulunurken, % 0.73’ü (4/546) BoHV-1 antikorları yönünden pozitifliği yansıtan gB(+)/gE(+) olarak belirlenmiştir. VNT ile serumların %31.86’ sı (174/546) CpHV-1, %3.29’u (18/546) BoHV-1 antikorları yönünden pozitif olarak değerlendirilmiştir. Sonuç olarak, örneklenen keçi sürülerinde CpHV1 enfeksiyonu prevalansı, BoHV-1 enfeksiyonundan yüksek bulunmuştur. Keçilerde alphaherpesvirus enfeksiyonlarının tanısında gB blocking ELISA alternatif test olarak kullanılabillir bulunmuş, ancak CpHV-1 ve BoHV-1 ayrımında gE blocking ELISA ve VNT kombinasyonunun kullanılması istatisitiksel (p<0.001) olarak uygun bulunmamıştır. Bu viruslar arasındaki antijenik çaprazreaksiyonları göz önünde bulundurarak, bu testlerdeki uyumsuzluklardan dolayı, CpHV-1 ve BoHV-1 antikorlarının belirlenmesi amacıyla daha hassas ve spesifik metotların geliştirilmesi önerilmektedir

Bovine herpesvirus 1 caprine herpesvirus 1 gB/gE ELISA goat virus neutralisation test

gB/gE ELISA keçi keçi herpesvirus 1 sığır herpesvirus 1 virus nötralizasyon testi

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