ankara univ vet fak derg

Ankara Üniversitesi Veteriner Fakültesi Dergisi

1300-0861 1308-2817

Ankara University

10.1501/Vetfak_0000002818

Veterinary Surgery

Veteriner Cerrahi

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Türkiye’de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü- 1 (PIT-1) veleptin (LEP) gen polimorfizmlerinin belirlenmesi

Bayram

Davut

Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine; Arslan

Korhan

Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine; Akyüz

Bilal

Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine; İşcan

Kaan Muhsin

Erciyes University, Department of Animal Breeding and Husbandary, Faculty of Veterinary Medicine;

12 01 2017

64 4 337 343 12 01 2017

1954

Ankara Üniversitesi Veteriner Fakültesi Dergisi

Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X2:40.75, p<0.001and X2:8.4, p<0.01)

Bu çalışmada Türkiye’de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç’lik ve LEP geni için 422 bç’lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X2:40.75, p<0.001ve X2:8.4, p<0.01)

Holstein leptin pit-I polymorphism RFLP

Holştayn leptin pit-1 polimorfizm RFLP

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Zhang L, Liu J, Zhao F, et al. (2013): Genome-wide association studies for growth and meat production traits in sheep. PloS one, 8, e66569. Geliş tarihi: 05.01.2016 / Kabul tarihi: 14.10.2016 Address for correspondence: Assist. Prof. Dr. Davut BAYRAM Erciyes University, Faculty of Veterinary Medicine, Department of Animal Breeding and Husbandary, Kayseri, Turkey. e-mail: vetdavut@gmail.com