ankara univ vet fak derg

Ankara Üniversitesi Veteriner Fakültesi Dergisi

1300-0861 1308-2817

Ankara University

10.1501/Vetfak_0000002666

Veterinary Surgery

Veteriner Cerrahi

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

Yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve moleküler tiplendirilmesi

Hanıfehnezhad

Alireza

Ankara University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Dışkapı, Ankara, Turkey. Göncüoğlu

Muammer

Ankara University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Dışkapı, Ankara, Turkey. Erol

İrfan

Ankara University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Dışkapı, Ankara, Turkey.

06 01 2015

62 2 113 117 06 01 2015

1954

Ankara Üniversitesi Veteriner Fakültesi Dergisi

The aim of the study was to determine the prevalance and molecular characterization of Clostridium perfringens in edible offal of broiler. For this purpose a total of 90 samples, including 45 heart-livers and 45 gizzards were obtained from different supermarkets and transported to the laboratory in an ice box and tested at the same day. Culture technique was used for the isolation and selected biochemical tests, including acid phosphatase and reverse-CAMP tests was used for the identification of C. perfringens from the samples. Twenty one (46.7%) of 45 heart-liver and eight (17.7%) of 45 gizzards were found to be contaminated with C. perfringens. Isolates were molecular characterized by using multiplex PCR with alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) genes. Results showed that all isolates were carrying cpa gene and named as type A but none of them were positive for cpe gene. Only two gizzard isolates were positive for cpb2 gene. Our results indicate that edible offal of broiler are highly contaminated with C. perfringens type A which is mostly responsible for the foodborne poisoning worldwide. According to the high contamination rate edible offal should be considered as one of the important causes of C. perfringens type A foodborne disease

Bu çalışmada yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve elde edilen izolatların moleküler karakterizasyonlarının belirlenmesi amaçlanmıştır. Çalışmada farklı süpermarketlerde satışa sunulan 45 kalp-karaciğer ve 45 taşlık olmak üzere toplam 90 yenilebilir iç organ soğuk zincir altında laboratuvara getirilerek aynı gün içerisinde analize alınmıştır. Örneklerden C. perfringens izolasyon ve identifikasyonunda klasik kültür tekniği ile asit fosfataz ve reverse-CAMP gibi biyokimyasal testler kullanılmıştır. Kırk beş kalp-karaciğer örneğinin 21’inin (% 46.7) ve 45 taşlık örneğinin sekizinin (% 17.7) C. perfringens ile kontamine olduğu saptanmıştır. Elde edilen izolatların alfa (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) ve enterotoksin (cpe) genlerine ait primer dizilimleri kullanılarak multipleks PCR ile moleküler tiplendirilmeleri yapılmıştır. Sonuçlar incelendiğinde bütün izolatların cpa geni taşıdığı belirlenmiş ve tip A olarak sınıflandırılmıştır, bununla beraber hiçbir izolatın cpe genine sahip olmadığı saptanmıştır. İki farklı taşlık örneğinden elde edilen izolatların cpa yanında cpb2 genini de taşıdıkları belirlenmiştir. Sonuç olarak yenilebilir piliç iç organlarının dünya genelinde gıda zehirlenmelerinde en sıklıkla rastlanan C. perfringens tip A ile yüksek oranda kontamine olduğu belirlenmiştir. Yüksek kontaminasyona bağlı olarak yenilebilir piliç iç organlarının C. perfringens tip A zehirlenmeleri açısından önemli birer kaynak oldukları saptanmıştır

Broiler edible offal Clostridium perfringens molecular typing multiplex PCR

Clostridium perfringens moleküler tiplendirme multipleks PCR yenilebilir piliç iç organ

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