ankara univ vet fak derg Ankara Üniversitesi Veteriner Fakültesi Dergisi 1300-0861 1308-2817 Ankara University 10.33988/auvfd.702257 Veterinary Surgery Veteriner Cerrahi The in vitro protein degradability of legume and sudan grass forage types and ensiled mixtures https://orcid.org/0000-0001-5057-1790 Stojanović Bojan University of Belgrade, Faculty of Agriculture, Department of Animal Science, Belgrade https://orcid.org/0000-0003-4196-4773 đorđević Nenad University of Belgrade, Faculty of Agriculture, Department of Animal Science, Belgrade https://orcid.org/0000-0002-7605-3796 Simić Aleksandar Belgrade University, Faculty of Agriculture, Department of Crop Science, Belgrade, https://orcid.org/0000-0002-8671-4906 Božičković Aleksa University of Belgrade, Faculty of Agriculture, Department of Animal Science, Belgrade https://orcid.org/0000-0003-0764-3183 Davidović Vesna University of Belgrade, Faculty of Agriculture, Department of Animal Science, Belgrade https://orcid.org/0000-0003-2762-1870 Ivetić Aleksandra University of Belgrade, Faculty of Agriculture, Department of Animal Science, Belgrade 09 01 2020 67 4 419 425 03 11 2020 06 09 2020 Copyright © 1954, Ankara Üniversitesi Veteriner Fakültesi Dergisi 1954 Ankara Üniversitesi Veteriner Fakültesi Dergisi

This study examined the in vitro crude protein (CP) degradability of wilted, dried for hay and ensiled Medicago sativa, Trifolium pratense and Sorghum sudanense, as well as of ensiled legume-sudan grass mixtures. The rumen degradable protein (RDP) was estimated using the latest Cornell net carbohydrate and protein system (CNCPS v6.5) and with the Streptomyces griseus protease assay (48 h of incubation). Sudan grass forages were the lowest in moderately degradable CP and the highest in CP fraction C, while red clover showed the least values for the soluble true protein like alfalfa forages for slowly degradable protein bound in neutral detergent fibre (NDF). Ensiling of legume-sudan grass mixtures decreases N-NH3 content by 12.2 and 5.1% in comparison with alfalfa and red clover silages, respectively. The RDP values for sudan grass (fresh, wilted, hay) were lower by 7.2 to 7.9% or 10.4 to 15.7% (CNCPS or S. griseus procedure) and up to 0.8 or 5.3 to 9.7% in comparison with alfalfa and red clover, respectively. The reduction of protein degradability in ensiled legume-sudan grass mixtures was 5.5 or 6.1% and 1.5 or 3% compared to alfalfa and red clover silages, respectively. Due to higher rumen undegradable protein content, the sudan grass and legume-sudan grass mixtures may be efficiently used to improve the protein utilization in ruminant nutrition.

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