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Effect of Different Storage Times and Temperatures on Pollen Viability of Hazelnut (Corylus avellana L.)

Year 2023, Volume: 12 Issue: Özel Sayı, 227 - 232, 29.10.2023
https://doi.org/10.29278/azd.1356668

Abstract

Objective: The aim of this study is to determine the most suitable pollen storage temperature for pollen grains belonging to 2 genotyes and 2 varieties of hazelnut (Corylus avellana L.) and to determine the most suitable genotype and variety for the pollen storage process.
Materials and Methods: Pollen grains belonging to the ‘Sarı’ and ‘Yomra’ genotyes and ‘Palaz’ and ‘Kara fındık’ species of Corylus avellana L. were collected from Akçakoca/Düzce, the second standard region of hazelnut production in Turkey, and then stored at 20 °C, 4 °C and -20 °C for 45 days. Pollen viability rates at 0., 15., 30. and 45. days were determined according to the fluorescein diacetate/propidium iodide protocol.
Results: Pollen viability rates of all genotypes decreased with increasing storage time and storage temperature. Storage at 20 °C resulted in a rapid decrease in pollen viability for all genotypes. Although storage at 4 °C provides adequate viability rates for all genotypes, the most appropriate storage temperature for all genotypes has been determined as -20 °C. It was determined that the most suitable genotype for pollen storage was 'Sarı' and the most suitable variety was 'Palaz’. Among all samples, the most suitable sample for storage was found to be the 'Sarı' genotype.
Conclusion: It is thought that the obtained findings may be helpful in studies related to artificial or complementary pollination processes in hazelnut and may contribute to the analysis of the limiting factors that occur in critical vital processes such as pollination and fertilization biology of the species.

References

  • Akond, A. M., Pounders, C. T., Blythe, E. K., & Wang, X. (2012). Longevity of crapemyrtle pollen stored at different temperatures. Scientia Horticulturae, 139, 53-57.
  • Aldahadha, A., Samarah, N., & Bataineh, A. (2020). Effect of storage temperature and duration on pollen viability and in vitro germination of seven pistachio cultivars. Journal of Applied Horticulture, 22(3), 111-114.
  • Ascari, L., Novara, C., Dusio, V., Oddi, L., & Siniscalco, C. (2020). Quantitative methods in microscopy to assess pollen viability in different plant taxa. Plant Reproduction, 33(3-4), 205-219.
  • Dafni, A., & Firmage, D. (2000). Pollen viability and longevity: practical, ecological and evolutionary implications. Pollen and Pollination, 113-132.
  • Dutta, S.K., Srivastav, M., Chaudhary, R., Lal, K., Patil, P., Singh, S. K., & Singh, A. K. (2013). Low temperature storage of mango (Mangifera indica L.) pollen. Scientia Horticulturae, 161, 193-197.
  • Ferrando, N. A., Montiel, F. G., & Burgos, L. (2007). Influence of storage temperature on teh viability of sweet cherry pollen. Spanish Journal of Agricultural Research, (1), 86-90.
  • Ge, Y., Fu, C., Bhandari, H., Bouton, J., Brummer, E.C., & Wang, Z.Y. (2011). Pollen viability and longevity of switchgrass (Panicum virgatum L.). Crop Science, 51(6), 2698-2705.
  • Hoekstra, F.A., & Bruinsma, J. (1975). Respiration and vitality of binucleate and trinucleate pollen. Physiologia Plantarum, 34(3), 221-225.
  • Impe, D., Reitz, J., Köpnick, C., Rolletschek, H., Börner, A., Senula, A., & Nagel, M. (2020). Assessment of pollen viability for wheat. Frontiers in Plant Science, 10, 1588.
  • İslam, A. (2018). Hazelnut culture in Turkey. Akademik Ziraat Dergisi, 7(2), 259-266.
  • İslam, A. (2019). Fındık ıslahında gelişmeler. Akademik Ziraat Dergisi, 8, 167-174.
  • Liu, J., Zhang, H., Cheng, Y., Kafkas, S., & Güney, M. (2014). Pistillate flower development and pollen tube growth mode during the delayed fertilization stage in Corylus heterophylla Fisch. Plant Reproduction, 27(3), 145-152.
  • Mehlenbacher, S.A., & Molnar, T.J. (2021). Hazelnut breeding. Plant Breeding Reviews, 45, 9-141.
  • Mesnoua, M., Roumani, M., & Salem, A. (2018). The effect of pollen storage temperatures on pollen viability, fruit set and fruit quality of six date palm cultivars. Scientia Horticulturae, 236, 279-283.
  • Novara, C., Ascari, L., La Morgia, V., Reale, L., Genre, A., & Siniscalco, C. (2017). Viability and germinability in long term storage of Corylus avellana pollen. Scientia Horticulturae, 214, 295-303.
  • Olsen, J. L., Mehlenbacher, S. A., & Azarenko, A. N. (2000). Hazelnut pollination. HortTechnology, 10(1), 113-115.
  • Shekari, A., Nazeri, V., & Shokrpour, M. (2016). Pollen viability and storage life in Leonurus cardiaca L. Journal of Applied Research on Medicinal and Aromatic Plants, 3(3), 101-104.
  • Singh, S. P., Singh, S. P., Pandey, T., Singh, R. R., & Sawant, S. V. (2015). A novel male sterility-fertility restoration system in plants for hybrid seed production. Scientific Reports, 5(1), 11274.
  • Toillon, J., Robin, J., Thomas, M., & Hamidi, R. (2023). Study on Nut Shell Lignification Progress in Hazelnut (Corylus avellana L.) cv. Segorbe. Journal of Nuts, 14(3), 191-199.
  • Vaknin, Y., & Eisikowitch, D. (2000). Effects of short‐term storage on germinability of pistachio pollen. Plant Breeding, 119(4), 347-350.

Farklı Saklama Süresi ve Sıcaklıklarının Fındıkta (Corylus avellana L.) Polen Canlılığı Üzerindeki Etkisi

Year 2023, Volume: 12 Issue: Özel Sayı, 227 - 232, 29.10.2023
https://doi.org/10.29278/azd.1356668

Abstract

Amaç: Bu çalışmanın amacı 2 fındık genotipi ve 2 fındık çeşidine (Corylus avellana L.) ait polen taneleri için en uygun polen saklama sıcaklığını belirlemek ve polen saklama işlemi için en uygun genotipi ve çeşidi tespit etmektir.
Materyal ve Yöntem: Corylus avellana L.’nın ‘Sarı’ ve ‘Yomra’ genotipleri ile, ‘Palaz’, ve ‘Kara fındık’ çeşitlerine ait polen taneleri Akçakoca/Düzce'den toplandıktan sonra 20 °C, 4 °C ve -20 °C’de 45 gün boyunca saklanmış ve 0., 15., 30. ve 45. günlerdeki polen canlılık oranları floresein diasetat/propidium iyodür protokolüne göre belirlenmiştir.
Araştırma bulguları: Tüm örneklerde polen canlılık oranları, saklama süresi ve saklama sıcaklığı arttıkça azalmıştır. 20 °C’de saklama polen canlılığının hızla azalmasına neden olmuştur. 4 °C’de saklama tüm örnekler için yeterli canlılık oranları sağlasa da en uygun saklama sıcaklığı -20 °C olarak belirlenmiştir. Polen saklama için en uygun olan genotipin ‘Sarı’, çeşidin ise ‘Palaz’ olduğu tespit edilmiştir. Tüm örnekler arasında saklama için en uygun örneğin ‘Sarı’ genotipi olduğu tespit edilmiştir.
Sonuç: Elde edilen bulguların fındıkta yapay ya da tamamlayıcı tozlaşma süreçleri ile ilgili çalışmalar için yararlı olabileceği ve türün tozlaşma ve döllenme biyolojisi gibi önemli yaşamsal süreçlerde ortaya çıkan kısıtlayıcı faktörlerin çözümlenmesine katkı sağlayabileceği düşünülmektedir.

References

  • Akond, A. M., Pounders, C. T., Blythe, E. K., & Wang, X. (2012). Longevity of crapemyrtle pollen stored at different temperatures. Scientia Horticulturae, 139, 53-57.
  • Aldahadha, A., Samarah, N., & Bataineh, A. (2020). Effect of storage temperature and duration on pollen viability and in vitro germination of seven pistachio cultivars. Journal of Applied Horticulture, 22(3), 111-114.
  • Ascari, L., Novara, C., Dusio, V., Oddi, L., & Siniscalco, C. (2020). Quantitative methods in microscopy to assess pollen viability in different plant taxa. Plant Reproduction, 33(3-4), 205-219.
  • Dafni, A., & Firmage, D. (2000). Pollen viability and longevity: practical, ecological and evolutionary implications. Pollen and Pollination, 113-132.
  • Dutta, S.K., Srivastav, M., Chaudhary, R., Lal, K., Patil, P., Singh, S. K., & Singh, A. K. (2013). Low temperature storage of mango (Mangifera indica L.) pollen. Scientia Horticulturae, 161, 193-197.
  • Ferrando, N. A., Montiel, F. G., & Burgos, L. (2007). Influence of storage temperature on teh viability of sweet cherry pollen. Spanish Journal of Agricultural Research, (1), 86-90.
  • Ge, Y., Fu, C., Bhandari, H., Bouton, J., Brummer, E.C., & Wang, Z.Y. (2011). Pollen viability and longevity of switchgrass (Panicum virgatum L.). Crop Science, 51(6), 2698-2705.
  • Hoekstra, F.A., & Bruinsma, J. (1975). Respiration and vitality of binucleate and trinucleate pollen. Physiologia Plantarum, 34(3), 221-225.
  • Impe, D., Reitz, J., Köpnick, C., Rolletschek, H., Börner, A., Senula, A., & Nagel, M. (2020). Assessment of pollen viability for wheat. Frontiers in Plant Science, 10, 1588.
  • İslam, A. (2018). Hazelnut culture in Turkey. Akademik Ziraat Dergisi, 7(2), 259-266.
  • İslam, A. (2019). Fındık ıslahında gelişmeler. Akademik Ziraat Dergisi, 8, 167-174.
  • Liu, J., Zhang, H., Cheng, Y., Kafkas, S., & Güney, M. (2014). Pistillate flower development and pollen tube growth mode during the delayed fertilization stage in Corylus heterophylla Fisch. Plant Reproduction, 27(3), 145-152.
  • Mehlenbacher, S.A., & Molnar, T.J. (2021). Hazelnut breeding. Plant Breeding Reviews, 45, 9-141.
  • Mesnoua, M., Roumani, M., & Salem, A. (2018). The effect of pollen storage temperatures on pollen viability, fruit set and fruit quality of six date palm cultivars. Scientia Horticulturae, 236, 279-283.
  • Novara, C., Ascari, L., La Morgia, V., Reale, L., Genre, A., & Siniscalco, C. (2017). Viability and germinability in long term storage of Corylus avellana pollen. Scientia Horticulturae, 214, 295-303.
  • Olsen, J. L., Mehlenbacher, S. A., & Azarenko, A. N. (2000). Hazelnut pollination. HortTechnology, 10(1), 113-115.
  • Shekari, A., Nazeri, V., & Shokrpour, M. (2016). Pollen viability and storage life in Leonurus cardiaca L. Journal of Applied Research on Medicinal and Aromatic Plants, 3(3), 101-104.
  • Singh, S. P., Singh, S. P., Pandey, T., Singh, R. R., & Sawant, S. V. (2015). A novel male sterility-fertility restoration system in plants for hybrid seed production. Scientific Reports, 5(1), 11274.
  • Toillon, J., Robin, J., Thomas, M., & Hamidi, R. (2023). Study on Nut Shell Lignification Progress in Hazelnut (Corylus avellana L.) cv. Segorbe. Journal of Nuts, 14(3), 191-199.
  • Vaknin, Y., & Eisikowitch, D. (2000). Effects of short‐term storage on germinability of pistachio pollen. Plant Breeding, 119(4), 347-350.
There are 20 citations in total.

Details

Primary Language Turkish
Subjects Plant Physiology, Plant Developmental and Reproductive Biology
Journal Section Makaleler
Authors

Özkan Kilin 0000-0002-9283-4576

Melse Su Bilgili 0000-0002-2062-7295

Aslıhan Çetinbaş Genç 0000-0001-5125-9395

Publication Date October 29, 2023
Published in Issue Year 2023 Volume: 12 Issue: Özel Sayı

Cite

APA Kilin, Ö., Bilgili, M. S., & Çetinbaş Genç, A. (2023). Farklı Saklama Süresi ve Sıcaklıklarının Fındıkta (Corylus avellana L.) Polen Canlılığı Üzerindeki Etkisi. Akademik Ziraat Dergisi, 12(Özel Sayı), 227-232. https://doi.org/10.29278/azd.1356668