baun health sci j Balıkesir Sağlık Bilimleri Dergisi 2146-9601 2147-2238 Balıkesir University SIÇANLARDA BEYİN PREFRONTAL KORTEKS DOKUSU ÜZERİNE OMEGA - 3 YAĞ ASİTLERİNİN KORUYUCU ETKİSİ THE PROTECTIVE EFFECTS OF OMEGA - 3 FATTY ACIDS ON PREFRONTAL CORTEX OF BRAIN IN RATS Gülcen Burak Balıkesir Üniversitesi Tıp Fakültesi, Anatomi Ana Bilim Dalı, Balıkesir Karaca Ömür Balıkesir Üniversitesi Tıp Fakültesi, Anatomi Ana Bilim Dalı, Balıkesir Kuş Murat Abdulgani Mehmet Akif Ersoy Üniversitesi, Sağlık Yüksek Okulu, Burdur Özcan Emrah Balıkesir Üniversitesi Tıp Fakültesi, Anatomi Ana Bilim Dalı, Balıkesir Kaman Dilara Fırat Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Elazığ Ögetürk Murat Fırat Üniversitesi Tıp Fakültesi, Anatomi Ana Bilim Dalı, Elazığ Kuş İlter Balıkesir Üniversitesi Tıp Fakültesi, Anatomi Ana Bilim Dalı, Balıkesir 04 30 2014 3 1 22 26 11 12 2013 02 20 2014 Copyright © 2012, Balıkesir Sağlık Bilimleri Dergisi 2012 Balıkesir Sağlık Bilimleri Dergisi

AMAÇ: Çalışmamızda prefrontal korteks dokusu üzerine omega - 3 yağ asitlerinin olumlu etkilerinin biyokimyasal düzeyde incelenmesi amaçlandı. YÖNTEMLER: Çalışmamız esnasında kullanılan 16 adet Wistar - Albino cinsi erişkin erkek sıçan iki ayrı gruba ayrıldı. Grup I’deki sıçanlar kontrol grubu olarak değerlendirildi ve intargastrik gavaj ile serum fizyolojik gün aşırı zerk edildi. Grup II’deki sıçanlara ise 0.4 g/kg dozdaki omega - 3 yağ asiti intragastrik gavaj yolu ile her gün verildi. Altı hafta devam eden deney süresi sonunda hayvanlar dekapite edilerek prefrontal korteks dokuları çıkarıldı. Alınan prefrontal korteks dokusu örneklerine ait süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH – Px) enzim aktivasyonları ile malondialdehit (MDA) düzeyleri spektrofotometrik olarak belirlendi. BULGULAR: Çalışmamız neticesinde omega - 3 yağ asiti verilen hayvanların prefrontal korteks dokularında SOD ve GSH – Px enzim aktiviteleri kontrol grubuna göre istatistiksel açıdan anlamlı bir artış gösterdi. MDA düzeyleri ise kontrol grubuna oranla anlamlı bir düşüş seyri gösterdi. SONUÇ: Sonuç olarak omega - 3 yağ asitlerinin beyin prefrontal korteks dokusundaki oksidatif hasara karşı önleyici bir etki yarattığı ve dolayısıyla bu doku üzerinde koruyucu bir işlev yürüttüğü tespit edildi.

OBJECTIVE: In our study of prefrontal cortex on the positive effects of omega - 3 fatty acids was aimed to investigate the biochemical level. METHODS: During our study used 16 Wistar - Albino male rats in the two groups were divided. Rats in Group I are used as control group and they are only intragastric gavaged with normal saline. Rats in group II, 0.4 g / kg dose of omega - 3 fatty acid was given every day by intragastric gavage. At the end of six weeks the ongoing experiments animals were decapitated and prefrontal cortex were removed. Get in the prefrontal cortex of superoxide dismutase (SOD) and glutathione peroxidase (GSH - Px) enzyme activation and malondialdehyde (MDA) levels were determined spectrophotometrically. RESULTS: As a result of our study of omega - 3 fatty acids in the prefrontal cortex of animals SOD and GSH - Px enzyme activity compared to the control group showed a statistically significant increase. MDA levels compared to the control group showed a significant downward trend. CONCLUSION: As a result, omega - 3 fatty acids in the prefrontal cortex of the brain, a preventive effect against oxidative damage and therefore conducted a protective function on these tissues were detected.

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