Endemik Astragalus microcephalus Wild Bitkisi Ekstraktının Ratlarda Deneysel Oluşturulan Yara Üzerine Etkisinin Araştırılması

Year 2023, , 104 - 110, 15.03.2023

Mahmut Şahin , Alper Serhat Kumru , Mustafa Özkaraca , Gökhan Yılmaz , Haki Kara

https://doi.org/10.53424/balikesirsbd.1077243

Abstract

ÖZ
Amaç: Bu çalışmanın amacı endemik Astragalus microcephalus wild (AST) bitkisi ekstraktının ratlarda deneysel oluşturulan yara üzerine etkisinin belirlenmesidir. Gereç ve Yöntem: Astragalus microcephalus. wild (AST) örneklerinden elde edilen ekstrakt, lanolin kullanılarak %2.5 ve %5’lik AST pomadı haline getirildi. Yine, ticari dekspantenol (Deks) (dekspantenol 500 mg/2ml, Bepanthen®) kullanılarak %2.5 ve %5’lik pomadlar oluşturuldu. Çalışmada her grupta 4 hayvan yer aldı.15 gün boyunca kontrol grubu hariç tüm hayvanlara pomad uygulaması günde bir kez yapıldı. Çalışmanın 3., 6., 9., 12. ve 15. günlerinde yara boyutları ölçüldü. 15. Günün sonunda dekapite edilerek yara bölgeleri histopatolojik inceleme için alındı. Bulgular: Epitelizasyon şekillenmesi, Mononükleer hücre infiltrasyonu ve granülasyon dokusu açısından gruplar arasında önemli farklılıklar (p <0.05) olduğu belirlendi. AST %2.5 ve AST 5 gruplarında ise epitel oluşumunun devam ettiği ve keratinleşmenin orta düzeylere yükseldiği belirlendi. Mn hücre infiltrasyonlarının Ast %5 grubunda hafiflediği görüldü. Granulasyon doku oluşumunun Dex %5, AST %2.5 ve AST %5 gruplarında olgunlaşmanın artmasıyla orta seviyede olduğu tespit edildi. Sonuç: Astragalus microcephalus wild ekstraktının yara iyileşmesi üzerine etkilerinin belirlenmesi amacıyla hazırlanan %2.5 ve % 5’lik pomatlarının yara iyileşmesini hızlı bir şekilde sağladığı görülmüştür.

Keywords

Astragalus, yara, rat

Supporting Institution

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Project Number

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Thanks

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Investigation of the Effect of Endemic Astragalus microcephalus Wild Plant Extract on Experimental Wound in Rats

Abstract

Objective: The aim of this study is to determine the effect of endemic Astragalus microcephalus wild (AST) plant extract on experimental wounds in rats. Materials and Methods: Astragalus microcephalus wild (AST) extract samples was turned into 2.5% and 5% AST ointment using lanolin. Again, 2.5% and 5% pomades were created using commercial dexpanthenol (Dex) (dexpanthenol 500 mg/2ml, Bepanthen®). There were 4 animals in each group in the study. For 15 days, ointment was applied to all animals except the control group once a day. Wound sizes were measured on the 3rd, 6th, 9th, 12th and 15th days of the study. At the end of the 15th day, the wound areas were removed for histopathological examination by decapitation. Results: It was determined that there were significant differences (p <0.05) between the groups in terms of epithelialization formation, Mononuclear cell infiltration and granulation tissue. It was determined that epithelial formation continued, and keratinization increased to moderate levels in AST 2.5% and AST 5 groups. Mn cell infiltrations were seen to be alleviated in the Ast 5% group. Granulation tissue formation was found to be moderate with increased maturation in Dex 5%, AST 2.5% and AST 5% groups. Conclusion: It has been observed that 2.5% and 5% pomades prepared to determine the effects of Astragalus microcephalus wild extract on wound healing provide rapid wound healing.

Keywords

Astragalus, wound, rat

Project Number

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