j. integr. anatol. med. Journal of Integrative and Anatolian Medicine 2687-6345 University of Health Sciences Pharmacology and Pharmaceutical Sciences Eczacılık ve İlaç Bilimleri Determination of Phenolic Compound Composition of Water and Ethanol Extracts of Horsetail (Equisetum arvense) At Kuyruğu (Equisetum arvense) Bitkisinin Su ve Etanol Ekstrelerinin Fenolik Bileşik İçeriğinin Belirlenmesi https://orcid.org/0000-0002-2500-1849 Ataseven Sibel SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE SAĞLIK BİLİMLERİ ENSTİTÜSÜ, GELENEKSEL VE TAMAMLAYICI TIP ANABİLİM DALI (DİSİPLİNLERARASI), GELENEKSEL VE TAMAMLAYICI TIP (YL) (TEZLİ) https://orcid.org/0000-0002-3581-4368 Mısırlı Duygu SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE ECZACILIK FAKÜLTESİ, HAMİDİYE ECZACILIK PR. https://orcid.org/0000-0002-8580-3712 Uzar Fatıma SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE SAĞLIK BİLİMLERİ ENSTİTÜSÜ, FİTOTERAPİ (DR) https://orcid.org/0000-0003-2549-9242 Türkan Nuran Neslinur SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE SAĞLIK BİLİMLERİ ENSTİTÜSÜ, GELENEKSEL VE TAMAMLAYICI TIP ANABİLİM DALI (DİSİPLİNLERARASI), GELENEKSEL VE TAMAMLAYICI TIP (YL) (TEZLİ) https://orcid.org/0000-0002-7149-7427 Elmastaş Mahfuz SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE ECZACILIK FAKÜLTESİ, HAMİDİYE ECZACILIK PR. 05 19 2021 2 2 3 9 04 21 2021 04 30 2021 Copyright © 2019, Journal of Integrative and Anatolian Medicine 2019 Journal of Integrative and Anatolian Medicine

Equisetum arvense, commonly known as horsetail, is generally used in traditional medicine as a therapeutic and pain reliever in wound, rheumatism, tuberculosis, skin diseases, hair loss, prostate, asthma, and urinary system diseases. Equisetum arvense contains many phenolic components. These components show high antioxidant activity.The aim of this study is to determine the phenolic compound composition of water and ethanol extracts of Equisetum arvense by High Performance Liquid Chromatography Equipped with Diode Array Detector (HPLC-DAD) which is sold by herbalists at the local markets and used for treatment in folk medicine.As a result of our study, the phenolic components of horsetail are found as 4-hydroxybenzoic acid (1.535 µg/kg DW), rutin (14.383 µg/kg DW), salicylic acid (9.639 µg/kg DW), vanillic acid (2.32 µg/kg DW) and chicoric acid (21.313 µg/kg DW) in water extract. Ethanol extract contains rutin (6.23695 µg/kg DW), quercetin (32.9995 µg/kg DW), gallic acid (0.95 µg/kg DW.), epicatechin (24.97 µg/kg DW), p-coumaric acid (5.97495 µg/kg DW), chicoric acid (39.98495 µg/kg DW), and cinnamic acid (1.7695 µg/kg DW).

Equisetum arvense, halk arasında bilinen adıyla atkuyruğu, geleneksel tıpta genellikle, yara, romatizma, tüberküloz, deri hastalıkları, saç dökülmesi, prostat, astım ve üriner sistem hastalıklarında tedavi edici ve ağrı kesici olarak kullanılır. Equisetum arvense yapısında birçok fenolik bileşen içerir bu bileşenler yüksek antioksidan aktivite gösterir. Bu çalışmanın amacı aktardan satın alınan atkuyruğu bitkisinin etanol ve su ekstrelerinin Diyot Array Detektörlü Yüksek Performans Sıvı Kromatografisi (HPLC-DAD) ile bireysel fenolik bileşik kompozisyonunun incelenmesidir. Çalışmamızın sonucunda atkuyruğunun halk arasında kullanılan şekli olan su ekstresinde fenolik bileşik miktarları kilogram kuru bitki başına; 4-hidroksibenzoik asit (1.535 µg/kg), rutin (14.383 µg/kg), salisilik asit (9.639 µg/kg,) vanilik asit (2.32 µg/kg) ve chicoric asit (21.313 µg/kg) olarak bulunurken etanol ekstresinde rutin (6.23695 µg/kg), quercetin (32.9995 µg/kg), gallik asit (0.95 µg/kg), epikatesin (24.97 µg/kg), p-kumarik asit (5.97495 µg/kg), chicoric asit (39.98495 µg/kg) ve sinamik asit (1.7695 µg/kg) olarak bulunmuştur.

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