Determination of Phenolic Compound Composition of Water and Ethanol Extracts of Horsetail (Equisetum arvense)
Abstract
Equisetum arvense, commonly known as horsetail, is generally used in traditional medicine as a therapeutic and pain reliever in wound, rheumatism, tuberculosis, skin diseases, hair loss, prostate, asthma, and urinary system diseases. Equisetum arvense contains many phenolic components. These components show high antioxidant activity.
The aim of this study is to determine the phenolic compound composition of water and ethanol extracts of Equisetum arvense by High Performance Liquid Chromatography Equipped with Diode Array Detector (HPLC-DAD) which is sold by herbalists at the local markets and used for treatment in folk medicine.
As a result of our study, the phenolic components of horsetail are found as 4-hydroxybenzoic acid (1.535 µg/kg DW), rutin (14.383 µg/kg DW), salicylic acid (9.639 µg/kg DW), vanillic acid (2.32 µg/kg DW) and chicoric acid (21.313 µg/kg DW) in water extract. Ethanol extract contains rutin (6.23695 µg/kg DW), quercetin (32.9995 µg/kg DW), gallic acid (0.95 µg/kg DW.), epicatechin (24.97 µg/kg DW), p-coumaric acid (5.97495 µg/kg DW), chicoric acid (39.98495 µg/kg DW), and cinnamic acid (1.7695 µg/kg DW).
The aim of this study is to determine the phenolic compound composition of water and ethanol extracts of Equisetum arvense by High Performance Liquid Chromatography Equipped with Diode Array Detector (HPLC-DAD) which is sold by herbalists at the local markets and used for treatment in folk medicine.
As a result of our study, the phenolic components of horsetail are found as 4-hydroxybenzoic acid (1.535 µg/kg DW), rutin (14.383 µg/kg DW), salicylic acid (9.639 µg/kg DW), vanillic acid (2.32 µg/kg DW) and chicoric acid (21.313 µg/kg DW) in water extract. Ethanol extract contains rutin (6.23695 µg/kg DW), quercetin (32.9995 µg/kg DW), gallic acid (0.95 µg/kg DW.), epicatechin (24.97 µg/kg DW), p-coumaric acid (5.97495 µg/kg DW), chicoric acid (39.98495 µg/kg DW), and cinnamic acid (1.7695 µg/kg DW).
Keywords
Equisetum arvense, Horsetail, Phenolic compound, Traditional medicine
Supporting Institution
Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Birimi
Project Number
2020/086
References
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