Araştırma Makalesi
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Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey

Yıl 2024, Cilt: 26 Sayı: 1, 249 - 259, 19.01.2024

Berna Sanön

https://doi.org/10.25092/baunfbed.1295744

Öz

Asteraceae, is a cosmopolitan family in terms of natural chemistry, flowering morphology, and adaptation to habitat. Even if there are numerous taxonomic and new molecular phylogenetic studies, the ancestors of the Asteraceae family are not well defined. Study material under consideration, Carlina L., is a member of the tribe Cardueae which belong to Cichorioideaea subfamily of the Asteraceae. The genus Carlina is represented by C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca in The Flora of Turkey. In this study, molecular phylogenetic analysis of the genus Carlina, which has a natural distribution in Turkey, has been made. The DNA isolation was performed using phenol- chloroform- isoamylalcohol. ITS (Internal Transcribed Region) of the nuclear ribosomal DNA (nrDNA) and the trnL-F (Transfer RNA coding) region of the chloroplast DNA sequences were used as molecular markers. Carlina was selected as ingroup taxon and Cardopatium, Atractylis and Carthamus were outgroup taxa. Taxa belonging to the genus Carlina distributed in Turkey were analyzed phylogenetically using Branch-and-Bound algorithm with maximum parsimony criterion. Data set analysis using 952 best parsimony trees was made. One tree topology was the most reliable and clads have strongly Bootstrap support.

Anahtar Kelimeler

asteraceae cardueae ITS trnLF Phylogenetic analysis

Proje Numarası

2011/58

Kaynakça

  • Heywood, V.H.. Flowering Plants of the World, Equinox Ltd Oxford UK 335, ISBN0709937784, (1985). https://doi.org/10.4236/oalib.1108387
  • Garcia-Jacas, N., Garnatje, T., Susana, A., Vilatersana, R., Tribal and Subtribal Delimitation and Phylogeny of the Cardueae (Asteraceae): A Combined Nuclear and Chloroplast DNA Analysis, Molecular Phylogenetic Evolution 22/1, January pp 5, (2002). https://doi.org/10.1006/mpev.2001.1038
  • Häffner, E., Hellwig, F.H., Phylogeny of the tribe Cardueae (Compositae) with emphasis on the subtribe Carduinae: an analysis based on ITS sequence data. Willdenowia 29, (1999). https://doi.org/10.3372/wi.29.2902
  • Davis, P.H., Flora of Turkey and the East Aegean Island, Edinburg University Press, Edinburg. 5, 597, (1975).
  • Dordević, S., Petrović, S., Dobrić, S., Milenković, M., Vučićević, D., Žižić, S., Kukić, J., Antimikrobial, anti-inflammatory, anti- ulcer and antioxidant activies of Carlina acanthifolia root essential oil, Journal Ethnopharmacol 109:458, (2007). https://doi.org/10.1016/j.jep.2006.08.021
  • Pavelaa, R., Maggic, F., Petrellic, R., Cappellaccic, L., Buccionic, M., Palmierid, A., Canalee, A., Benellie, G., Outstanding insecticidal activity and sublethal effects of Carlina acaulis root essential oil on the housefly, Musca domestica, with insights on its toxicity on human cells, Food and Chemical Toxicology 136: 111037, (2020). https://doi.org/10.1016/j.fct.2019.111037
  • Kadereit, J.W., Jeffre C. (eds), Flowering Plants, Eudicots:Asterales, In Kubitzki K(ed). The Families and Genera of Vascular Plants, vol.8 Springer: Verlag Berlin, (2007).
  • Daniele, C., Dahamna, S., Firuzi, O., Sekfali, N., Saso, L., Mazzati, G, Atractylis gummufera L. poisining: an ethnopharmacological review, Journal Ethnopharmacol, 97:175, (2005). https://doi.org/10.1016/j.jep.2004.11.025
  • Wolfe, K.H., Li, W.H., Sharp, P.M., Rates of nucleotide substitutions vary greatly among plant mitockontrial cloroplast, and nuclear DNAs, Proceedings of the National Academy of Sciences, USA, 84:9054, (1987). https://doi.org/10.1073/pnas.84.24.9054
  • Gruenstaeudl, M., Urtubey, E., Jansen, R.K., Samuel, R., Barfuss, M.H.J., Stuessy, T.F., Phylogeny of Barnadesioideae (Asteraceae) inferred from DNA sequence data and morphology. Molecular Phylogenetics and Evolution 51:572, (2009). https://doi.org/10.1016/j.ympev.2009.01.023
  • Hickey, L.J., Wolfe, J.A., The Bases of Angiosperm Phylogeny: Vegetative Morphology, Annals of the Missouri Botanical Garden 62/3:538, (1975). https://doi.org/10.2307/2395267
  • Batır, B., Türkiye’de Carlina L. cinsine ait taksonların (Asteraceae) polen morfolojisi, Çanakkale Onsekiz Mart University Institute of Science Master's Thesis, (2009).
  • Punt, W., Hoen, P.P., The Northwest European Pollen Flora 70 Asteraceae- Asteroideae, Peview of Palaeobotany and Palynology, 157:22, (2009).
  • Osman, A.K., Contribution to the polen morphology of Tribe Cardueae (Cichorioideae- Compositae), Feddes Repertorium 120:145, (2009). https://doi.org/10.1002/fedr.200811103
  • Jong, T.J., Klinkhamer, P.G.L., Heiden, J.L.H., The effect of water and mycorrhiza infection on the distribution of Carlina vulgaris on sand dunes, Ecography 18:384, (1995). https://doi.org/10.1111/j.1600-0587.1995.tb00141.x
  • Balos, M.M., Akan, H., Flora of the Region between Zeytinbahçe and Akarçay (Birecik, Şanlıurfa, Turkey), Turkish Journal Botany, 32:201, (2008).
  • Sheppard, A.W., Vito J., The effect of a rosette-crown fly Botanophila turcica on growth biomass allocation and reproduction of the thistle Carthamus lanatus. Acta Oecologica 21/6:337, (2000). https://doi.org/10.1016/S1146-609X(00)01090-0
  • Dellaporte, S.L.,Wood, J., Hicks, J.B., A Plant DNA minipreparation: Version II, Plant Molecular Biology Reporter,1/4, 19, (1983).
  • Taberlet, P., Gielly, L., Pautou, G., Bouvet, J., Universal Primers for Amplification Three Non-coding Regions of Chloroplast DNA, Plant Molecular Biology 17:1105, (1991). https://doi.org/10.1007/BF00037152
  • Spaniols, S., Bazakos, C., Spano, T., Zoghby, C., Kalaitzis, P., The potential of plastid trnL (UAA) intron polymorphisms fort the identification of the botanical origin of plant oils, Food Chemistry 122:850, (2010). https://doi.org/10.1016/j.foodchem.2010.02.039
  • Susanna, A., Garcia-Jacas, N., Hidalgo, O., Vilatersana, R., Garnadje, T., The Cardueae (Compositae) Revisited: Insights from ITS trnL-trnF and matK Nuclear and Cloroplast DNA Analysis, Annals of the Missouri Botanical Garden 93:150, (2006). (https://doi.org/10.3417/0026- 6493(2006)93
  • White, T.J., Bruns, T., Lee, S. and Taylor, J., Amplifications and direct sequencing of fungal ribosomal RNA genes for phylogenetics In Innis M, Gelfand D, Sninsky J and White T (eds) PCR protocols: a guide to methods and applications. Academic Pres San Diego California, USA, 315, (1990).
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice, Nucleic Acid Research 22: 4673, (1994). https://doi.org/10.1093/nar/22.22.4673
  • Wofford, D.L., Phylogenetic Analysis Using Parsimony and Other Methods In (PAUP* 4.0b10) Ed 4.0b10, Smithsonian Institution&Sinauer Associates Washington D.C.& Sunderland MA p Computer Program for Phylogenetic Analysis, (2005).
  • Felsenstein, J., Confidence limits on phylogenies: An approach using bootstrap, Evolution 39:783, (1985). https://doi.org/10.2307/2408678
  • Sanön, B., Yıldız, B., Coşkun, F., Türkiye’de Yetişen Carlina L. (Asteraceae, Cardueae) Türlerinin Morfolojik Filogenetik Analizi, BAÜ Fen Bilimleri Enstitüsü Dergisi, Cilt 13(2):50-60, (2011). https://doi.org/10.1016/j.fct.2019.111037
  • Wahrmund, U., Heklau, H., Röser, M., Kästner, A., Vitek, E., Ehrendorfer, F. K., Bernhard von Hagen, F., A molecular phylogeny reveals frequent changes of growth form in Carlina (Asteraceae), Taxon 59(2): 367–378, (2010). https://doi.org/10.2307/25677596

Türkiye'deki Carlina L. (Asteraceae) cinsine ait taksonların moleküler filogenetik analizi

Yıl 2024, Cilt: 26 Sayı: 1, 249 - 259, 19.01.2024

Berna Sanön

https://doi.org/10.25092/baunfbed.1295744

Öz

Asteraceae, doğal kimyası, çiçeklenme morfolojisi ve habitata uyumu açısından kozmopolit bir familyadır. Çok sayıda taksonomik ve yeni moleküler filogenetik çalışma olsa da, Asteraceae familyasının ataları tam olarak tanımlanmamıştır. İncelenen çalışma materyali, Carlina L., Asteraceae'nin Cichorioideaea alt familyasına ait Cardueae kabilesinin bir üyesidir. Carlina cinsi, Türkiye Florasında C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca ile temsil edilir. Bu çalışmada, Türkiye'de doğal yayılış gösteren Carlina cinsinin moleküler filogenetik analizi yapılmıştır. DNA izolasyonu fenol-kloroform-izoamilalkol kullanılarak yapıldı. Moleküler markör olarak nükleer ribozomal DNA'nın (nrDNA) ITS (Internal Transkripsiyonlu Bölge) ve kloroplast DNA sekanslarının trnL-F (Transfer RNA kodlama) bölgesi kullanıldı. İç grup taksonu olarak Carlina, dış grup taksonu olarak Cardopatium, Atractylis ve Carthamus seçilmiştir. Türkiye'de yayılış gösteren Carlina cinsine ait taksonlar, maksimum parsimoni kriteri ile Branch-and-Bound algoritması kullanılarak filogenetik olarak analiz edilmiştir. 952 en iyi parsimoni ağacı kullanılarak veri seti analizi yapılmıştır. Bir ağaç topolojisi en güvenirlikte ve dallar güçlü bir şekilde Bootstrap desteğine sahiptir.

Anahtar Kelimeler

Asteraceae Cardueae ITS trnL-F Filogenetik Analiz

Destekleyen Kurum

Balıkesir Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

2011/58

Kaynakça

  • Heywood, V.H.. Flowering Plants of the World, Equinox Ltd Oxford UK 335, ISBN0709937784, (1985). https://doi.org/10.4236/oalib.1108387
  • Garcia-Jacas, N., Garnatje, T., Susana, A., Vilatersana, R., Tribal and Subtribal Delimitation and Phylogeny of the Cardueae (Asteraceae): A Combined Nuclear and Chloroplast DNA Analysis, Molecular Phylogenetic Evolution 22/1, January pp 5, (2002). https://doi.org/10.1006/mpev.2001.1038
  • Häffner, E., Hellwig, F.H., Phylogeny of the tribe Cardueae (Compositae) with emphasis on the subtribe Carduinae: an analysis based on ITS sequence data. Willdenowia 29, (1999). https://doi.org/10.3372/wi.29.2902
  • Davis, P.H., Flora of Turkey and the East Aegean Island, Edinburg University Press, Edinburg. 5, 597, (1975).
  • Dordević, S., Petrović, S., Dobrić, S., Milenković, M., Vučićević, D., Žižić, S., Kukić, J., Antimikrobial, anti-inflammatory, anti- ulcer and antioxidant activies of Carlina acanthifolia root essential oil, Journal Ethnopharmacol 109:458, (2007). https://doi.org/10.1016/j.jep.2006.08.021
  • Pavelaa, R., Maggic, F., Petrellic, R., Cappellaccic, L., Buccionic, M., Palmierid, A., Canalee, A., Benellie, G., Outstanding insecticidal activity and sublethal effects of Carlina acaulis root essential oil on the housefly, Musca domestica, with insights on its toxicity on human cells, Food and Chemical Toxicology 136: 111037, (2020). https://doi.org/10.1016/j.fct.2019.111037
  • Kadereit, J.W., Jeffre C. (eds), Flowering Plants, Eudicots:Asterales, In Kubitzki K(ed). The Families and Genera of Vascular Plants, vol.8 Springer: Verlag Berlin, (2007).
  • Daniele, C., Dahamna, S., Firuzi, O., Sekfali, N., Saso, L., Mazzati, G, Atractylis gummufera L. poisining: an ethnopharmacological review, Journal Ethnopharmacol, 97:175, (2005). https://doi.org/10.1016/j.jep.2004.11.025
  • Wolfe, K.H., Li, W.H., Sharp, P.M., Rates of nucleotide substitutions vary greatly among plant mitockontrial cloroplast, and nuclear DNAs, Proceedings of the National Academy of Sciences, USA, 84:9054, (1987). https://doi.org/10.1073/pnas.84.24.9054
  • Gruenstaeudl, M., Urtubey, E., Jansen, R.K., Samuel, R., Barfuss, M.H.J., Stuessy, T.F., Phylogeny of Barnadesioideae (Asteraceae) inferred from DNA sequence data and morphology. Molecular Phylogenetics and Evolution 51:572, (2009). https://doi.org/10.1016/j.ympev.2009.01.023
  • Hickey, L.J., Wolfe, J.A., The Bases of Angiosperm Phylogeny: Vegetative Morphology, Annals of the Missouri Botanical Garden 62/3:538, (1975). https://doi.org/10.2307/2395267
  • Batır, B., Türkiye’de Carlina L. cinsine ait taksonların (Asteraceae) polen morfolojisi, Çanakkale Onsekiz Mart University Institute of Science Master's Thesis, (2009).
  • Punt, W., Hoen, P.P., The Northwest European Pollen Flora 70 Asteraceae- Asteroideae, Peview of Palaeobotany and Palynology, 157:22, (2009).
  • Osman, A.K., Contribution to the polen morphology of Tribe Cardueae (Cichorioideae- Compositae), Feddes Repertorium 120:145, (2009). https://doi.org/10.1002/fedr.200811103
  • Jong, T.J., Klinkhamer, P.G.L., Heiden, J.L.H., The effect of water and mycorrhiza infection on the distribution of Carlina vulgaris on sand dunes, Ecography 18:384, (1995). https://doi.org/10.1111/j.1600-0587.1995.tb00141.x
  • Balos, M.M., Akan, H., Flora of the Region between Zeytinbahçe and Akarçay (Birecik, Şanlıurfa, Turkey), Turkish Journal Botany, 32:201, (2008).
  • Sheppard, A.W., Vito J., The effect of a rosette-crown fly Botanophila turcica on growth biomass allocation and reproduction of the thistle Carthamus lanatus. Acta Oecologica 21/6:337, (2000). https://doi.org/10.1016/S1146-609X(00)01090-0
  • Dellaporte, S.L.,Wood, J., Hicks, J.B., A Plant DNA minipreparation: Version II, Plant Molecular Biology Reporter,1/4, 19, (1983).
  • Taberlet, P., Gielly, L., Pautou, G., Bouvet, J., Universal Primers for Amplification Three Non-coding Regions of Chloroplast DNA, Plant Molecular Biology 17:1105, (1991). https://doi.org/10.1007/BF00037152
  • Spaniols, S., Bazakos, C., Spano, T., Zoghby, C., Kalaitzis, P., The potential of plastid trnL (UAA) intron polymorphisms fort the identification of the botanical origin of plant oils, Food Chemistry 122:850, (2010). https://doi.org/10.1016/j.foodchem.2010.02.039
  • Susanna, A., Garcia-Jacas, N., Hidalgo, O., Vilatersana, R., Garnadje, T., The Cardueae (Compositae) Revisited: Insights from ITS trnL-trnF and matK Nuclear and Cloroplast DNA Analysis, Annals of the Missouri Botanical Garden 93:150, (2006). (https://doi.org/10.3417/0026- 6493(2006)93
  • White, T.J., Bruns, T., Lee, S. and Taylor, J., Amplifications and direct sequencing of fungal ribosomal RNA genes for phylogenetics In Innis M, Gelfand D, Sninsky J and White T (eds) PCR protocols: a guide to methods and applications. Academic Pres San Diego California, USA, 315, (1990).
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice, Nucleic Acid Research 22: 4673, (1994). https://doi.org/10.1093/nar/22.22.4673
  • Wofford, D.L., Phylogenetic Analysis Using Parsimony and Other Methods In (PAUP* 4.0b10) Ed 4.0b10, Smithsonian Institution&Sinauer Associates Washington D.C.& Sunderland MA p Computer Program for Phylogenetic Analysis, (2005).
  • Felsenstein, J., Confidence limits on phylogenies: An approach using bootstrap, Evolution 39:783, (1985). https://doi.org/10.2307/2408678
  • Sanön, B., Yıldız, B., Coşkun, F., Türkiye’de Yetişen Carlina L. (Asteraceae, Cardueae) Türlerinin Morfolojik Filogenetik Analizi, BAÜ Fen Bilimleri Enstitüsü Dergisi, Cilt 13(2):50-60, (2011). https://doi.org/10.1016/j.fct.2019.111037
  • Wahrmund, U., Heklau, H., Röser, M., Kästner, A., Vitek, E., Ehrendorfer, F. K., Bernhard von Hagen, F., A molecular phylogeny reveals frequent changes of growth form in Carlina (Asteraceae), Taxon 59(2): 367–378, (2010). https://doi.org/10.2307/25677596
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Morfolojisi ve Anatomisi
Bölüm Araştırma Makalesi
Yazarlar

Berna Sanön 0000-0002-4343-770X

Proje Numarası 2011/58
Erken Görünüm Tarihi 6 Ocak 2024
Yayımlanma Tarihi 19 Ocak 2024
Gönderilme Tarihi 11 Mayıs 2023
Yayımlandığı Sayı Yıl 2024 Cilt: 26 Sayı: 1

Kaynak Göster

APA Sanön, B. (2024). Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26(1), 249-259. https://doi.org/10.25092/baunfbed.1295744
AMA Sanön B. Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey. BAUN Fen. Bil. Enst. Dergisi. Ocak 2024;26(1):249-259. doi:10.25092/baunfbed.1295744
Chicago Sanön, Berna. “Molecular Phylogenetic Analysis of the Taxa Belonging to the Genus Carlina L.(Asteraceae) in Turkey”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 26, sy. 1 (Ocak 2024): 249-59. https://doi.org/10.25092/baunfbed.1295744.
EndNote Sanön B (01 Ocak 2024) Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 26 1 249–259.
IEEE B. Sanön, “Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey”, BAUN Fen. Bil. Enst. Dergisi, c. 26, sy. 1, ss. 249–259, 2024, doi: 10.25092/baunfbed.1295744.
ISNAD Sanön, Berna. “Molecular Phylogenetic Analysis of the Taxa Belonging to the Genus Carlina L.(Asteraceae) in Turkey”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 26/1 (Ocak 2024), 249-259. https://doi.org/10.25092/baunfbed.1295744.
JAMA Sanön B. Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey. BAUN Fen. Bil. Enst. Dergisi. 2024;26:249–259.
MLA Sanön, Berna. “Molecular Phylogenetic Analysis of the Taxa Belonging to the Genus Carlina L.(Asteraceae) in Turkey”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 26, sy. 1, 2024, ss. 249-5, doi:10.25092/baunfbed.1295744.
Vancouver Sanön B. Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey. BAUN Fen. Bil. Enst. Dergisi. 2024;26(1):249-5.