Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method

Year 2017, Volume: 7 Issue: 2, 80 - 84, 26.12.2017

Elif Kaval Oğuz

https://doi.org/10.17678/beuscitech.331471

Abstract

In this study, it was aimed to prove the way of in
vitro conditioned cells death. There is an increased axonal regeneration after a
peripheral nerve is injured if it exposed to an earlier prior injury. The first
injury causing the enhanced regeneration is called a conditioning lesion. In
this study, Dorsal Root Ganglion primer cell culture was performed then neurons
were conditioned in vitro with an effective and new method. It was observed that in
vitro conditioned cells were regenerated much faster than control group cells
but at the end of 48^th hour, they died. The cell death way of these
in vitro conditioned neurons was determined by an in situ Cell Detection Kit Fluorescein and visualized by
fluorescent microscopy. Finally, apoptotic cell deaths observed at in vitro conditioned neurons. This study
is important for the continuity of enhanced regeneration capacity of
conditioning lesion effect and neuronal survival and also it will provide the
basis for new studies.

Keywords

Dorsal root ganglion (DRG), regeneration, conditioning lesion

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