Türkiye için lokal endemik bir tür olan mahmuzsuz Delphinium turcicum (Ranunculaceae)'un morfolojik, anatomik, palinolojik, tohum mikromorfolojik çalışmaları ve taksonomik önemi

Year 2024, Volume: 17 Issue: 1, 7 - 17, 15.04.2024

Yağmur Cömert , Osman Tugay , Deniz Ulukuş

https://doi.org/10.46309/biodicon.2023.1293145

Abstract

Bu çalışmada Türkiye endemiği olan Delphinium turcicum (Ranunculaceae) türünün morfolojik, anatomik, palinolojik, mikromorfolojik ve taksonomik önemi araştırılmıştır. Ayrıca Delphinium turcicum'un benzer bir türü olan Delphinium venolusum çalışılmıştır. Bu türlerin anatomik yapılarının tanımları ve Delphinium turcicum taksonu için tohum tanımları ilk kez bu çalışmada verilmiştir. Ek olarak bu türlerin karşılaştırılması taksonomik problemlerin çözümüne katkı sağlamaktadır. Bu makalede anatomik yapıların (kök, gövde ve yaprak kısımları) özellikleri gösterilmiştir. Ayrıca bu yapıların taksonomik önemi tartışılmıştır. Bu çalışmada D. turcicum ve D. venulosum arasındaki benzerlikler ve farklılıklar ortaya konmuştur. Palinolojik araştırmalarda; polar, ekvatoral, ekzin, intin, kolpus uzunluk ve genişlikleri ölçülmüştür. Polar/Ekvatoral (P/E) oranı ve polen ornamentasyonu belirlenmiştir. İncelenen bu iki türün polenleri sferik (küresel) ve izopolar simetriye sahiptir. Polen tipi D. turcicum ve D. venulosum türleri için monad olarak tespit edilmiştir. Polen apertür tipleri trikolpattır. Polen ekzin ornamentasyonları ise mikroekinattır. Bu çalışmada ışık mikroskobu ve taramalı elektron mikroskobu (SEM) kullanılarak polen ve tohum fotoğrafları çekilmiştir. Tohum mikromorfolojik özellikleri verilmiştir. Tohumlar subglobozdur ve her iki tür için de sürekli enine skuamüloz halkalara sahiptir. Tohum ornamentasyonları ise, sürekli enine skuamüloz halkalara ve genellikle dik papillalara sahiptir.

Keywords

Anadolu, anatomi, morfoloji, palinoloji, Pseudodelphinium, taksonomi

The morphological, anatomical, palynological, seed micromorphological studies and its taxonomical significance in Delphinium turcicum (Ranunculaceae), a local endemic spurless species for Turkey

Abstract

In this study, species of Delphinium turcicum (Ranunculaceae), which is endemic to Türkiye, for its morphological, anatomical, palynological, micromorphological and taxonomical significance was investigated. Additionally, Delphinium venolusum, which is a similar species of Delphinium turcicum, was studied. Anatomic structure descriptions of these species and seed descriptions of Delphinium turcicum were given for the first time in this work. In addition, the comparison of these species contributes to the solution of taxonomic problems. Features of anatomic structures (root, stem, and leaf sections) were shown. Also, these structures were discussed taxonomic significance. The similarities and differences between D. turcicum and D. venulosum were revealed in this study. In palynological investigations; polar, equatorial, exine, intine, colpus length and width were measured. Polar/Equatorial (P/E) ratio and pollen ornamentation were determined. The pollen grains of these two species are spherical and isopolar symmetrical. Pollen types have determined as monad for D. turcicum and D. venulosum species. Pollen aperture types are tricolpate. Pollen exine ornamentations are microechinate. In this work photographs of pollen and seed were taken by using light microscopy and scanning electron microscopy (SEM). Seed micromorphological characteristics were given for both species. Seeds are subglobose and have continuous rings of transversal squamulose for both species. Seed ornamentations have continuous rings of transversal squamulose and generally erect papillae.

Keywords

Anatolia, anatomy, morphology, palynology, Pseudodelphinium, taxonomy

Thanks

This article is derived from Master Thesis: ‘Palinological, morphological and anatomical characteristics of the endemic genus Pseudodelphinium (Ranunculaceae) in Turkey’. The authors received no financial support for the research and thesis. I would like to thank S. Karagöz (Konya Selçuk University) and Research Asst. E. Karahisar (Konya Selçuk University) for helping during the laboratory studies.

References

• [1] Tamura, M. (1963). Morphology, ecology and phylogeny of Ranunculaceae, Sci. Rep. Coll. Gen. Education Osaka Univ. 13(1):25-38.
• [2] Chartier, M., Dressler, S., Schönenberger, J., Rojas Mora, A., Sarthou, C., Wang, W. & Jabbor, F. (2016). The evolution of afro-montane Delphinium (Ranunculaceae): Morphospecies, phylogenetics and biogeography. Taxon, 65(6), 1313-1327. https://doi.org/10.12705/656.6
• [3] Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M. T. (edlr.) (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler) [Türkiye plant list (Vascular plants)]. Türkiye (pp. 1-1290). Flora Araştırmaları Derneği ve Nezahat Gökyiğit Botanik Bahçesi Yayınları, İstanbul.
• [4] Jabbour, F. & Renner, S.S. (2011). Consolida and Aconitella are an annual clade of Delphinium (Ranunculaceae) that diversified in the Mediterranean basin and Irano-Turanian region. Taxon, 60(4), 1029-1040. https://doi.org/10.1002/tax.604007
• [5] İlarsan, H., İlarsan, R., & Blanché, C. (1997). Seed morphology of the genus Delphinium L. (Ranunculaceae) in Türkiye. Collectanea Botanica, 1997 vol. 23, p. 79-95. https://doi.org/10.3989/collectbot.1997.v23.68
• [6] Trifonova, V.I. (1990) Comparative biomorphological study of the taxonomy and phylogeny of the genera Consolida (DC.) S.F. Gray and Aconitella Spach. Collectanea Botanica, (19), 97-110.
• [7] Hadidchi, A. Attar, F. & Ullah, F. (2020). Using microscopic techniques for taxonomic implications of seed and fruits of Delphinium L.(sensu lato)(Ranunculaceae). Microscopy Research and Technique, 83(2), 99-117. https://doi.org/10.1002/jemt.23393
• [8] De Leeuw, A., Bukowski, K., Krijgsman, W. & Kuiper, K. F. (2010). Age of the badenian salinity crisis; impact of Miocene climate variability on the circum-Mediterranean region. Geology, 38(8), 715-718. https://doi.org/10.1130/G30982.1
• [9] Davis, P. H., Cullen, J., & Coode, M. J. E. (1965). Ranunculaceae. Flora of Türkiye and The East Aegean Islands, vol. 1, pp. 94-134.
• [10] Rewicz, A., Bomanowska, A., Magda, J. & Rewicz, T. (2017). Morphological variability of Consolida regalis seeds of south-eastern and central Europe. Systematics and Biodiversity, 15(1), 25-34. https://doi.org/10.1080/14772000.2016.1216017
• [11] Vural, M., Duman, H., Aytac, Z. & Adıgüzel, N. (2012). A new genus and three new species from Central Anatolia, Türkiye. Turkish Journal of Botany, 36(5): 427- 433.
• [12] Espinosa, F. Deroin, T. Xiang, K.-L. Wang, W. Castro, M. P. Byng, J. W. Aytaç, Z. Nadot, S. & Jabbour, F. (2017). The Turkish endemic Pseudodelphinium turcicum (Ranunculaceae): an unusual population of Delphinium with peloric flowers that has persisted in the wild for 20 years. International Journal of Plant Sciences, 178 (7), 000-000. https://doi.org/10.1086/692764
• [13] Espinosa, F. & Myreya, P.C. (2018). On the use of herbarium specimens for morphological and anatomical research. Botany Letters, 165(3-4), 361-367. https://doi.org/10.1080/23818107.2018.1451775
• [14] Espinosa, F., Deroin, T., Malécot, V., Wang, W., Pinedo, M., Nadot, S., & Jabbour, F. (2021). Historical note on the taxonomy of the genus Delphinium L.(Ranunculaceae) with an amended description of its floral morphology. Adansonia, 43(2), 9-18. https://doi.org/10.5252/adansonia2021v43a
• [15] Xiang, K.L. Aytaç, Z. Liu, Y. Espinosa, F. Jabbour, F. Byng, J. W. & Wang, W. (2017). Recircumscription of Delphinium subg. Delphinium (Ranunculaceae) and implications for its biogeography. Taxon, 66(3), 554-566. https://doi.org/10.12705/663.3
• [16] Ullah, F. Zafar, M. Ahmad, M. Dilbar, S. Shah, S. N. Sohail, A. & Tariq, A. (2018). Pollen morphology of subfamily Caryophylloideae (Caryophyllaceae) and its taxonomic significance. Microscopy Research and Technique, 81(7), 704-715. https://doi.org/10.1002/ jemt.23026
• [17] Ullah, F. Papini, A. Shah, S.N. Zaman, W. Sohail, A. & Iqbal, M. (2019). Seed micromorphology and its taxonomic evidence in subfamily Alsinoideae (Caryophyllaceae). Microscopy Research and Technique, 82 (3), 250-259. https://doi.org/10.1002/jemt.23167
• [18] Johansen, D.A. (1940). Plant microtechique. Inc; London: McGraw-Hill Book Company.
• [19] Wodehouse, R.P. (1935). Pollen grains. Their structure, identification and significance in science and medicine. New York, NY. London: Mcgraw-Hill Book Company.
• [20] Özdamar, K. (2004). Paket Programlar ile İstatistiksel Veri Analizi-1. Eskişehir: Kaan Kitabevi.
• [21] Punt, W. Hoen, P. Blackmore, S. Nilsson, S. & Le Thomas, A. (2007). Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology, 143(1-2), 1-81.
• [22] Stearn, W.T. (1983). Botanical Latin. David & Charles Pub, London.
• [23] Stace, C.A. (1991). Plant taxonomy and Biosystematics. Cambridge, UK: Cambridge University Press.
• [24] Kolar, F. Pai, S.R. & Dixit, G.B. (2017). Delphinium malabaricum (Huth) Munz: A potential ornamental crop from Western Ghats. Horticulture International Journal, 1(1), 16-21. https://doi.org/ 10.15406/hij.2017.01.00003