biotech studies Biotech Studies 2687-3761 2757-5233 Tarla Bitkileri Merkez Araştırma Enstitüsü Nanomedicine Nanoilaç Design of PLGA nanofiber wound dressing enriched with Echinacea purpurea extract and in vitro characterization https://orcid.org/0000-0002-5657-5490 Şenol Büşranur EGE UNIVERSITY, INSTITUTE OF SCIENCE, DEPARTMENT OF BIOMEDICAL TECHNOLOGIES https://orcid.org/0000-0002-4235-3942 Malbora Ayşe EGE UNIVERSITY, INSTITUTE OF SCIENCE, DEPARTMENT OF BIOMEDICAL TECHNOLOGIES https://orcid.org/0000-0002-5972-8798 Şenol Mehmet Emin EGE UNIVERSITY, INSTITUTE OF SCIENCE, DEPARTMENT OF BIOMEDICAL TECHNOLOGIES https://orcid.org/0000-0001-8954-9996 Morçimen Zehra Gül EGE UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF BIOENGINEERING, BIOENGINEERING PR. 04 24 2026 35 1904228 01 11 2025 12 23 2025 Copyright © 1992, Biotech Studies 1992 Biotech Studies

Electrospun nanofiber wound dressings potentially provide a highly suitable environment for healing compared to traditional wound dressings, which often fail to meet all the requirements of the wound healing process. This study aimed to develop PLGA nanofibers incorporating silver nanoparticles containing Echinacea extract for use as wound dressings; to perform their characterization tests, and evaluate their wound healing efficacy using in vitro models. Echinacea silver nanoparticles were synthesized using a green synthesis method. PLGA, silver nanoparticle containing PLGA (AgNP/PLGA), 1%, 2%, 3%, Echinacea silver nanoparticle containing PLGA (Ec-AgNP/PLGA) nanofibers were produced by electrospinning method. The nanofibers were characterized by SEM, FTIR, XRD and antibacterial analysis and evaluated using an in vitro wound scratch test. The average zone diameters of Ec-AgNP/PLGA nanofibers against the E. coli and S. aureus bacteria were found to be 13.5±0.3 mm. The antioxidant capacity analysis of the Echinacea extract used in the study was evaluated by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and the percentage of DPPH radical removal was calculated as 46.8% and the ascorbic acid equivalent was determined as 35.5 µM. The results demonstrated that Echinacea significantly accelerated wound healing due to its therapeutic and antioxidant properties.

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