In vitro sensitivity of anthracnose disease agent, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., to some fungicides on lemon
Abstract
Anthracnose is one of the diseases that seriously
threaten citrus production, especially lemons in the world and in our country. The
aim of this study was to determine the sensitivity of an anthracnose disease
agent, Colletotrichum
gloeosporioides, on lemon, to some fungicides with different
mechanisms of action from different chemical groups such as DMI, SDHI, QoI,
amides, anilines and dithiocarbamates. For this purpose, tissue pieces of
diseased plants collected from lemon orchards were incubated in PDA medium at
25 °C for 5 days. All isolates were identified as Colletotrichum gloeosporioides as a result of morphological and
molecular diagnoses of fungal cultures. The virulence levels of all isolates were determined in
pathogenicity tests and the highest virulence was found to have LC5 isolate.
The susceptibility of C. gloeosporioides
LC5 isolate to 10 different fungicides was demonstrated by in vitro mycelial growth and conidial germination trials. As a
result, it was determined that fungicides fluopyram+tebuconazole, fluazinam,
fluxapyroxad+ difenoconazole and dithianon+phosphorous acid showed the highest
effect.
Keywords
References
- Alexander SA, Waldenmaier CM. Management of Anthracnose in Bell Pepper. Fungicide and Nematicide Tests . Vol. 58. New Fungicide and Nematicide Data Committee of the American Phytopathological Society; 2002. p. 49.
- Bosland, P.W., Votana, E.J.,2003. Peppers: Vegetable and Spice Capsicums. CAB International, England,p.233.FAO, 2016. Statistics of Food and Agriculture Organization of the United Nations, 2016
- Gao,Y.Y., He, L.F., Li, B.X., Mu, W., Lin, J., Liu, F., 2017. Sensitivity of Colletotrichum acutatum to six fungicides and reduction in incidence and severity of chili anthracnose using pyraclostrobin Australasian Plant Patholology. (2017) 46:521–528.
- Gao, Y., He, L., Mu, W., Li, B., Lin,J., Liu, F., 2018. Assessment of the baseline sensitivity and resistance risk of Colletotrichum acutatum to fludioxonil. European Journal of Plant Pathology (2018) 150:639–651.
- Guarnaccia, V. Groenewald, J.Z. Polizzi, G. Crous, P.W. 2017. High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia 39, 2017: 32–50.
- Hadden, J.F., Black, L.L., 1989. Anthracnose of pepper caused by Colletotrichum spp. Proceeding of the International Symposium on Integrated Management Practices: Tomato and peper production in the tropics. Asian Vegetable Research and Development Centre. Taiwan, p.189-199.
- Harp T, Kuhn P, Roberts PD, Pernezny KL. 2014. Management and cross-infectivity potential of Colletotrichum acutatum causing anthracnose on bell pepper in Florida. Phytoparasitica. 2014;42:31–39.
- Ishii, H., Zhen, F., Hu, M., Lib, X., Schnabel, G., 2016. Efficacy of SDHI fungicides, including benzovindiflupyr, against Colletotrichum species. Pest Management Science 2016; 72: 1844–1853.
- Kloutvorova, J., Egert, P., 2004. New possibilities for protecting stone fruits with the fungicide Horizon 250 EW. Online Courier
- Lewis IML, Miller SA. Evaluation of Fungicides and a Biocontrol Agents for the Control of Anthracnose on Green Pepper Fruit, 2003. Nematicide Test Report. Vol. 58. New Fungicide and Nematicide Data Committee of the American Phytopathological Society; 2003. p. 62.
- Piccirillo, G., Carrieria, R., Polizzib, G., Azzaroc, A., Lahoza, E., Fernández-Ortuñod, D., Vitaleb, A., 2018. In vitro and in vivo activity of QoI fungicides against Colletotrichum gloeosporioides causing fruit anthracnose in Citrus sinensis. Scientia Horticulturae 236 (2018) 90–95.
- Ramos AP, Talhinhas P, Sreenivasaprasad S, Oliveira, H., 2016. Characterization of Colletotrichum gloeosporioides, as the main causal agent of citrus anthracnose, and C. karstii as species preferentially associated with lemon twig dieback in Portugal. Phytoparasitica 44: 549–561.
- Vieira, W.A.S., Lima, W.G., Nascimento, E.S., Michereff, S.J., Reis, A., Doyle, V.P., Camara, M.P.S., 2017. Thiophanate Methyl Resistance and Fitness Components of Colletotrichum musae isolates from Banana in Brazil. Plant Disease. 101.1659-1665.
Limonda Antraknoz hastalığı etmeni Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.’in bazı fungisitlere karşı in vitro duyarlılığı
Abstract
Antraknoz,
dünyada ve ülkemizde limon başta olmak üzere turunçgil üretimini ciddi düzeyde
tehdit eden hastalıklardan birisidir. Bu çalışmanın amacı, limonda
Antraknoz hastalığı etmeni Colletotrichum gloeosporioides’in DMI, SDHI, QoIs, amid, aniline ve
dithiocarbamate gibi farklı kimyasal gruplardan farklı etki mekanizmalarına
sahip bazı fungisitlere karşı duyarlılığını belirlemektir.
Bu
amaçla, limon bahçelerinden toplanan hastalıklı bitkilerin doku parçaları, PDA
besi yerinde 25 °C’de 5 gün inkübe edilmiştir. Fungal kültürlerin morfolojik ve
moleküler teşhisleri sonucunda tüm izolatların, Colletotrichum
gloeosporioides olduğu belirlenmiştir. Patojenisite denemelerinde tüm izolatların
virülenslik düzeyleri belirlenmiş ve en yüksek virülensliğe LC5 izolatının
sahip olduğu tespit edilmiştir. C. gloeosporioides LC5 izolatının 10 farklı fungisite karşı
duyarlılıkları, in vitro’da miselyal
gelişim ve konidiyal çimlenme denemeleri ile ortaya konmuştur. Sonuç olarak en
yüksek etkiyi, fluopyram+tebuconazole, fluazinam, fluxapyroxad+difenoconazole
ve dithianon+fosforoz asit fungisitlerinin gösterdiği belirlenmiştir.
Keywords
References
- Alexander SA, Waldenmaier CM. Management of Anthracnose in Bell Pepper. Fungicide and Nematicide Tests . Vol. 58. New Fungicide and Nematicide Data Committee of the American Phytopathological Society; 2002. p. 49.
- Bosland, P.W., Votana, E.J.,2003. Peppers: Vegetable and Spice Capsicums. CAB International, England,p.233.FAO, 2016. Statistics of Food and Agriculture Organization of the United Nations, 2016
- Gao,Y.Y., He, L.F., Li, B.X., Mu, W., Lin, J., Liu, F., 2017. Sensitivity of Colletotrichum acutatum to six fungicides and reduction in incidence and severity of chili anthracnose using pyraclostrobin Australasian Plant Patholology. (2017) 46:521–528.
- Gao, Y., He, L., Mu, W., Li, B., Lin,J., Liu, F., 2018. Assessment of the baseline sensitivity and resistance risk of Colletotrichum acutatum to fludioxonil. European Journal of Plant Pathology (2018) 150:639–651.
- Guarnaccia, V. Groenewald, J.Z. Polizzi, G. Crous, P.W. 2017. High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia 39, 2017: 32–50.
- Hadden, J.F., Black, L.L., 1989. Anthracnose of pepper caused by Colletotrichum spp. Proceeding of the International Symposium on Integrated Management Practices: Tomato and peper production in the tropics. Asian Vegetable Research and Development Centre. Taiwan, p.189-199.
- Harp T, Kuhn P, Roberts PD, Pernezny KL. 2014. Management and cross-infectivity potential of Colletotrichum acutatum causing anthracnose on bell pepper in Florida. Phytoparasitica. 2014;42:31–39.
- Ishii, H., Zhen, F., Hu, M., Lib, X., Schnabel, G., 2016. Efficacy of SDHI fungicides, including benzovindiflupyr, against Colletotrichum species. Pest Management Science 2016; 72: 1844–1853.
- Kloutvorova, J., Egert, P., 2004. New possibilities for protecting stone fruits with the fungicide Horizon 250 EW. Online Courier
- Lewis IML, Miller SA. Evaluation of Fungicides and a Biocontrol Agents for the Control of Anthracnose on Green Pepper Fruit, 2003. Nematicide Test Report. Vol. 58. New Fungicide and Nematicide Data Committee of the American Phytopathological Society; 2003. p. 62.
- Piccirillo, G., Carrieria, R., Polizzib, G., Azzaroc, A., Lahoza, E., Fernández-Ortuñod, D., Vitaleb, A., 2018. In vitro and in vivo activity of QoI fungicides against Colletotrichum gloeosporioides causing fruit anthracnose in Citrus sinensis. Scientia Horticulturae 236 (2018) 90–95.
- Ramos AP, Talhinhas P, Sreenivasaprasad S, Oliveira, H., 2016. Characterization of Colletotrichum gloeosporioides, as the main causal agent of citrus anthracnose, and C. karstii as species preferentially associated with lemon twig dieback in Portugal. Phytoparasitica 44: 549–561.
- Vieira, W.A.S., Lima, W.G., Nascimento, E.S., Michereff, S.J., Reis, A., Doyle, V.P., Camara, M.P.S., 2017. Thiophanate Methyl Resistance and Fitness Components of Colletotrichum musae isolates from Banana in Brazil. Plant Disease. 101.1659-1665.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Makaleler |
| Authors | |
| Publication Date | March 29, 2019 |
| Submission Date | October 16, 2018 |
| Acceptance Date | December 10, 2018 |
| Published in Issue | Year 2019 Volume: 59 Issue: 1 |
