Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 2147-3129 2147-3188 Bitlis Eren University 10.17798/bitlisfen.1782134 Biomolecular Modelling and Design Biyomoleküler Modelleme ve Tasarım Synthesis, Structure Elucidation, Antioxidant, Molecular Docking, Molecular Dynamic Simulation, and MM-PBSA Analysis of Isatin Derivatives Bearing Thiosemicarbazone https://orcid.org/0000-0002-4428-4696 Yakan Hasan ONDOKUZ MAYIS ÜNİVERSİTESİ https://orcid.org/0000-0002-1979-5427 Yenigün Semiha ONDOKUZ MAYIS UNIVERSITY https://orcid.org/0000-0001-8306-2378 Muğlu Halit KASTAMONU UNIVERSITY https://orcid.org/0000-0003-0133-5630 Ozen Tevfik ONDOKUZ MAYIS UNIVERSITY 03 24 2026 15 1 137 158 09 11 2025 02 22 2026 Copyright © 2012, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 2012 Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

A series of new isatin derivatives bearing thiosemicarbazone (1–8) were obtained using various isatins and N-(2-isopropylphenyl)hydrazinecarbothioamide. The intermediated thiosemicarbazide was obtained by the reaction of 1-isopropyl-2-isothiocyanatobenzene with hydrazine monohydrate. The structure and purity of all newly synthesized compounds were confirmed through standard analytical techniques, including 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and elemental analysis. In this study, novel isatin-thiosemicarbazone derivatives were synthesized and structurally characterized using spectroscopic methods. Their antioxidant activities were evaluated by the DPPH˙ scavenging assay. Furthermore, the interactions of the compounds with urease enzyme were investigated through molecular docking and molecular dynamics simulations. The findings indicate that certain compounds exhibit promising biological activity in terms of both antioxidant potential and urease inhibition. The compounds 5 and 3 can be used as inhibitors in the treatment of urease-induced gastric disorders. These results provide valuable insights into the biological relevance of the synthesized molecules.

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