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Hedera helix L. Türünün Sürünücü ve Tırmanıcı Kısımlarında Bazı Pigment ve Antosiyanin İçeriklerinin Karşılaştırılması

Year 2019, Volume: 8 Issue: 3, 749 - 754, 30.09.2019
https://doi.org/10.17798/bitlisfen.522166

Abstract

Hedera
helix
L.
yaprak döken orman ekosistemlerinin önemli türlerinden birisidir. Yenice
Ormanları’nda bulunan Kavaklı Tabiatı Koruma Alanı’nda tırmanıcı özelliğinin
dışında yoğun bir yer örtücü (sürünücü) özellik de sergilemektedir. Bu
çalışmada, Kavaklı Tabiatı Koruma Alanı’nda yayılış gösteren H. helix
türünün sürünücü ve tırmanıcı kısımlarının yaprak ve gövdelerinde bazı bitki
pigmentleri (klorofil-a, klorofil-b, Kl-a/Kl-b, karotenoid ve antosiyanin)
açısından fark olup olmadığının araştırılması amaçlanmıştır. Üç farklı bitki
bireyinden yaprak ve gövde örnekleri toplanmış ve 1 g taze örnek kullanılarak
ekstraksyon işlemi yapılmıştır. Klorofiller için 662, 645 nm ve karetenoid için
470 nm’deki absorbanslar ölçülmüştür. Antosiyanin miktarını belirlemek için 1 g
yaprak örneği 12 ml metanol/HCl (99/1) içerisinde 24 saat +4 0C’de
inkübe edilerek 530 ile 657 nm’deki absorbanslar ölçülmüştür. Sonuçların
değerlendirilmesinde tek yönlü varyans analizi (ANOVA) ve Tukey testi ile
kullanılmıştır. Buna göre, sürünücü ve tırmanıcı bitki kısımlarının yaprak ve
gövdeleri arasında Kla, Klb, Kla/Klb ve karotenoid içerikleri açısından
istatistiksel olarak bir farklılık bulunmamıştır. Antosiyanin içeriği açısından
farklı bitki kısımlarına ait gövdeler arasında önemli bir farklılık bulunmazken,
tırmanıcı kısımlardaki yapraklarda sürünücü kısımların yapraklarına göre
anlamlı düzeyde daha yüksek olduğu tespit edilmiştir (P<0,05). Elde edilen
bulgular, yaprak döken orman ekosistemlerinde farklı orman katlarında bulunan H.
helix
türünün hem sürünücü formda hem de tırmanıcı formda biyomas ve
oksijen üretimi açısından benzer etkililikte katkı sağladığını göstermektedir. 

References

  • 1. Chamberlain D.F. 1972. Hedera L., in Flora of Turkey and the East Aegean Islands, Edited by Davis P.H., Vol.4, Edinburgh: Edinburgh Univ Press, 538-539.
  • 2. Metcalfe D.J. 2005. Hedera helix L., Journal of Ecology, 93(3): 632-648.
  • 3. Ellenberg H. 1988. Vegetation Ecology of Central Europe. Cambridge University Press, 731s. Cambridge, UK.
  • 4. Sack L., Grubb P.J. 2002. The combined impacts of deep shade and drought on the growth and biomass allocation of shade-tolerant woody seedlings, Oecologia, 131, 175–185.
  • 5. Castagneri D., Garbarino M., Nola P. 2013. Host preference and growth patterns of ivy (Hedera helix L.) in a temperate alluvial forest, Plant Ecology, 214(1): 1-9.
  • 6. Rogers S.O., Bonnett H.T. 1989. Evidence for apical initial cells in the vegetative shoot apex of Hedera helix cv. Goldheart, American Journal of Botany, 76(4): 539-545.
  • 7. Scheres B. 2001. Plant cell identity. The role of position and lineage, Plant Physiology, 125(1): 112-114.
  • 8. De Kok L.J., Graham M. 1989. Level of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark-induced and natural senescence, Plant Physiology and Biochemistry, 27: 203-209.
  • 9. Lichtenthaler H.K., Wellburn A.R. 1983. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents, Biochemical Society Transaction, 11: 591-592.
  • 10. Mancinelli A.L., Yang C.P.H., Lindquist P., Anderson O.R., Rabino I. 1975. Photocontrol of anthocyanin synthesis: III. The action of streptomycin on the synthesis of chlorophyll and anthocyanin, Plant Physiology, 55(2): 251-257.
  • 11. Reay F.P., Fletcher R.H., Thomas V.J.G. 1998. Chlorophylls, carotenoids and anthocyanin concentrations in the skin of ‘Gala’ apples during maturation and the influence of foliar applications of nitrogen and magnesium, Journal of the Science of Food Agriculture, 76: 63-71.
  • 12. Biggerstaff M.S., Beck C.W. 2007. Effects of English ivy (Hedera helix) on seed bank formation and germination, The American Midland Naturalist, 157: 250-257.
  • 13. Murray J.R., Hacket W.P. 1991. Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L., Plant Physiology, 97343-351.
  • 14. Murray J.R., Smith A.G., Hackett W.P. 1994. Differential dihydroflavonol reductase transcription and anthocyanin pigmentation in the juvenile and mature phases of ivy (Hedera helix L.), Planta, 194: 102-109.
  • 15. Dale M.P., Causton D.R. 1992. Use of the chlorophyll a/b ratio as a bioassay for the light environment of a plant, Functional Ecology, 80(3): 190-196.
  • 16. Poethig R.S. 2003. Phase change and the regulation of developmental timing in plants, Science, 301(5631): 334-336.
  • 17. Kerstetter R.A., Poethig R.S. 1998. The specification of leaf identity during shoot development, Annual Review of Cell and Developmental Biology, 14(1), 373-398.
  • 18. Hutchison K.W., Sherman C.D., Weber J., Smith S.S., Singer P.B., Greenwood M.S. 1990. Maturation in larch. II. Effects of age on photosynthesis and gene expression in developing foliage, Plant Physiology, 94: 1308-1315.
  • 19. Cevahir C, Yentür S, Yazgan M, Ünal M, Yılmazer N 2004. Peroxidase activity in relation to anthocyanin and chlorophyll content in juvenile and adult leaves of “mini-star” Gazania splendens, Pakistan Journal of Botany 36(3): 603-609.
  • 20. Bauer H., Bauer U. 1980. Photosynthesis in leaves of the juvenile and adult phase of ivy (Hedera helix), Physiologia Plantarum, 49(4): 366-372.
  • 21. Grace S.C. 2005. Phenolics as antioxidants, in Antioxidants and Reactive Oxygen Species in Plants, Edited by Smirnoff N., Blackwell Publishing Ltd, UK, 141-168.
Year 2019, Volume: 8 Issue: 3, 749 - 754, 30.09.2019
https://doi.org/10.17798/bitlisfen.522166

Abstract

References

  • 1. Chamberlain D.F. 1972. Hedera L., in Flora of Turkey and the East Aegean Islands, Edited by Davis P.H., Vol.4, Edinburgh: Edinburgh Univ Press, 538-539.
  • 2. Metcalfe D.J. 2005. Hedera helix L., Journal of Ecology, 93(3): 632-648.
  • 3. Ellenberg H. 1988. Vegetation Ecology of Central Europe. Cambridge University Press, 731s. Cambridge, UK.
  • 4. Sack L., Grubb P.J. 2002. The combined impacts of deep shade and drought on the growth and biomass allocation of shade-tolerant woody seedlings, Oecologia, 131, 175–185.
  • 5. Castagneri D., Garbarino M., Nola P. 2013. Host preference and growth patterns of ivy (Hedera helix L.) in a temperate alluvial forest, Plant Ecology, 214(1): 1-9.
  • 6. Rogers S.O., Bonnett H.T. 1989. Evidence for apical initial cells in the vegetative shoot apex of Hedera helix cv. Goldheart, American Journal of Botany, 76(4): 539-545.
  • 7. Scheres B. 2001. Plant cell identity. The role of position and lineage, Plant Physiology, 125(1): 112-114.
  • 8. De Kok L.J., Graham M. 1989. Level of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark-induced and natural senescence, Plant Physiology and Biochemistry, 27: 203-209.
  • 9. Lichtenthaler H.K., Wellburn A.R. 1983. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents, Biochemical Society Transaction, 11: 591-592.
  • 10. Mancinelli A.L., Yang C.P.H., Lindquist P., Anderson O.R., Rabino I. 1975. Photocontrol of anthocyanin synthesis: III. The action of streptomycin on the synthesis of chlorophyll and anthocyanin, Plant Physiology, 55(2): 251-257.
  • 11. Reay F.P., Fletcher R.H., Thomas V.J.G. 1998. Chlorophylls, carotenoids and anthocyanin concentrations in the skin of ‘Gala’ apples during maturation and the influence of foliar applications of nitrogen and magnesium, Journal of the Science of Food Agriculture, 76: 63-71.
  • 12. Biggerstaff M.S., Beck C.W. 2007. Effects of English ivy (Hedera helix) on seed bank formation and germination, The American Midland Naturalist, 157: 250-257.
  • 13. Murray J.R., Hacket W.P. 1991. Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L., Plant Physiology, 97343-351.
  • 14. Murray J.R., Smith A.G., Hackett W.P. 1994. Differential dihydroflavonol reductase transcription and anthocyanin pigmentation in the juvenile and mature phases of ivy (Hedera helix L.), Planta, 194: 102-109.
  • 15. Dale M.P., Causton D.R. 1992. Use of the chlorophyll a/b ratio as a bioassay for the light environment of a plant, Functional Ecology, 80(3): 190-196.
  • 16. Poethig R.S. 2003. Phase change and the regulation of developmental timing in plants, Science, 301(5631): 334-336.
  • 17. Kerstetter R.A., Poethig R.S. 1998. The specification of leaf identity during shoot development, Annual Review of Cell and Developmental Biology, 14(1), 373-398.
  • 18. Hutchison K.W., Sherman C.D., Weber J., Smith S.S., Singer P.B., Greenwood M.S. 1990. Maturation in larch. II. Effects of age on photosynthesis and gene expression in developing foliage, Plant Physiology, 94: 1308-1315.
  • 19. Cevahir C, Yentür S, Yazgan M, Ünal M, Yılmazer N 2004. Peroxidase activity in relation to anthocyanin and chlorophyll content in juvenile and adult leaves of “mini-star” Gazania splendens, Pakistan Journal of Botany 36(3): 603-609.
  • 20. Bauer H., Bauer U. 1980. Photosynthesis in leaves of the juvenile and adult phase of ivy (Hedera helix), Physiologia Plantarum, 49(4): 366-372.
  • 21. Grace S.C. 2005. Phenolics as antioxidants, in Antioxidants and Reactive Oxygen Species in Plants, Edited by Smirnoff N., Blackwell Publishing Ltd, UK, 141-168.
There are 21 citations in total.

Details

Primary Language Turkish
Journal Section Araştırma Makalesi
Authors

Emire Elmas 0000-0002-5620-1798

Sevda Türkiş 0000-0002-1853-8437

Publication Date September 30, 2019
Submission Date February 4, 2019
Acceptance Date April 21, 2019
Published in Issue Year 2019 Volume: 8 Issue: 3

Cite

IEEE E. Elmas and S. Türkiş, “Hedera helix L. Türünün Sürünücü ve Tırmanıcı Kısımlarında Bazı Pigment ve Antosiyanin İçeriklerinin Karşılaştırılması”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 8, no. 3, pp. 749–754, 2019, doi: 10.17798/bitlisfen.522166.

Bitlis Eren University
Journal of Science Editor
Bitlis Eren University Graduate Institute
Bes Minare Mah. Ahmet Eren Bulvari, Merkez Kampus, 13000 BITLIS