Myrtus communis L. (Mersin) Yaprak Ekstraktının Genoprotektif Etkisinin Somatik Mutasyon ve Rekombinasyon Testi ile Değerlendirilmesi

Year 2019, Volume: 8 Issue: 3, 875 - 882, 30.09.2019

Selda Öz Şükran Çakır Arıca

https://doi.org/10.17798/bitlisfen.577518

Abstract

Bu çalışmada Myrtus communisL. (mersin) yaprak ekstraktının genoprotektif etkisi somatik mutasyon ve rekombinasyon testi (SMART) ile incelendi. Yüksek genotoksik etkiye sahip kemoterapötik bir ajan olan doksorubisin (DXR) pozitif kontrol olarak kullanıldı. Test maddeleri flare (flr³) ve çoklu kanat kılı (mwh) mutant işaret genlerini taşıyan üç günlük (72 ± 4 saat) transheterozigot Drosophila melanogaster larvalarına uygulandı. Mersin yaprak ekstraktı, genotoksik etkisini değerlendirmek için tek başına (1,5 ve 10 mg/mL) ve antigenotoksik etkisini değerlendirmek için doksorubisin (0,125 mg/ml) ile birlikte uygulandı. İnhibisyon yüzdeleri 1, 5 ve 10 mg/mL dozlarında sırasıyla %91.70, % 97.51 ve % 98.34 olarak hesaplandı. Bu çalışmadan elde edilen sonuçlar mersin yaprak ekstraktının test edilen tüm dozlarda doksorubisin kaynaklı mutant klon oluşumunu inhibe ederek antigenotoksik etki gösterdiğini ortaya koymuştur. 

Keywords

Myrtus communis L., antigenotoksisite, doksorubisin, Drosophila melanogaster

Supporting Institution

Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2012/010

Thanks

Bu çalışma Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje No: 2012/010).

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