Research Article
BibTex RIS Cite

Determination of Degrees of Oxidative Stress and Some Antioxidant Enzyme Activities in Basketball Players

Year 2019, Volume: 8 Issue: 4, 1296 - 1301, 24.12.2019
https://doi.org/10.17798/bitlisfen.642861

Abstract

Amaç: Fiziksel aktivite kalp hastalıklarının
tedavi ve önlenmesinde önemli bir faktör olduğu bilinmektedir. Akut egzersiz,
oksidatif stresin serbest radikal oluşumunun büyük bir kaynağının olduğu rapor
edilmektedir. Bu çalışmada basketbol oyuncularında, oksidatif stres düzeyi ve
bazı antioksidant enzim aktivite düzeylerinin 
saptanması  amaçlanmıştır. Metot:
Çalışmamızda, Basketbol oynayan 14 erkek sporcu ile 14 sedanter birey çalışmaya
alındı. Çalışmaya katılan bireylerin, yağ asitlerinin, serbest radikallerle
reaksiyonu sonucu oluşan peroksidasyon ürünlerinden, malondialdehit (MDA),
katalaz (CAT) aktivitesi ve Redükte glutatyon (GSH) düzeyleri spektrofotometrik
olarak ölçümler yapılmıştır. Sonuçlar: Basketbol sporcuları ile kontrol
grubunun, MDA, CAT ve GSH düzeyleri arasında istatistiksel olarak anlamlı fark bulunmuştur
(p<0.05). Önemli bir antioksidant enzim aktivitesi olan Katalaz ve MDA
değerleri  Basketbolcularda kontrol
grubuna göre önemli bir azalma meydana gelirken, aktif olarak oynayan
Basketbolcularda , GSH değeri  kontrole
grubundan daha yüksek bulunmuştur (p<0.05).  Sonuç olarak, Basketbol takımında yer alan ve
aktif olarak oynayan sporcularda, Katalaz ve MDA da azalma görülürken, GSH da
ise  artış bulunmuştur. Çok güçlü bir
antioksidant enzim olan katalaz enzim aktivitesinin yoğun egzersiz yapan bireylerde
oksidatif hasara karşı iyi bir koruyucu olduğu söylenebilir

References

  • Aebi H [1984] Catalase.(in) Methots In Enzymology, L. Packer(Editör), 105: 121-126, Academic Pres, Orlando.
  • Aguiló A, Tauler P, Gimeno I, Fuentespina E, Pons A.[2000] Changes in erythrocyte antioxidant enzymes during prolonged submaximal exercise. Biofactors.11(1-2):27-30.
  • Akkuş İ. [1995] Serbest Radikaller ve Fizyopatolojik Etkileri(Free Radicals and Their Physiopathologic Effects). Baskı 1. Konya, Mimoza Basım – Yayım ve Dağıtım A.Ş, a. 49-50.
  • Alptekin Ö, Yıldırım D, Özyılmaz G ve Tokel S [2004] Florosile immobilize edilmiş katalaz enziminin immobilizasyon koşullarının optimizasyonu, XVIII. National Chemistry Congree, BK, S. 498, Kars
  • Alvying A, Carson P, Flanagan CL, Ickes C, [1956] Enzymatic deficiency in primaquin-sensitive erythrocytes. Science, 14, (124):484-5.
  • Arslan A, Tüzün FA, Arslan H, Demir H, Tamer S, Demir C, Tasin M. [2016] The relationship between serum paraoxonase levels and carotid atherosclerotic plaque formation in Alzheimer's patients. Neurol Neurochir Pol. Dec;50(6):403-409.
  • Asker S, Asker M, Sarikaya E, Sunnetcioglu A, Aslan M, Demir H.[2015] Oxidative stress parameters and their correlation with clinical, metabolic and polysomnographic parameters in severe obstructive sleep apnea syndrome. Int J Clin Exp Med. 2015 Jul 15;8(7):11449-55.
  • Aslan R, Şekeroğlu MR.[1996] Egzersize bağlı lipid peroksidasyonu ve antioksidan statüsü çalışmalarında sonuçlara etkili faktörler. Turkish Journal of Sports Medicine, 31: 145-152.
  • Atli M. [2013] Serum paraoxonase activity and lipid hydroperoxide levels in adult football players after three days football tournament, African Health Sciences Vol 13 Issue 3 September
  • Banerjee AK, Mandal A, Chanda D, Chakraborti S.[2003] Oxidant, antioxidant and physical exercise. Mol Cell Biochem.253(1-2):307-12.
  • Beutler E., Duron O., Kelly BM., [1963] Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882‐888
  • Bilici M, Cim N, Demir H. [2014] Pre and post-operative oxidative stress level in cases with ovarian cancer neoplasia. Medical Science and Discovery, Vol. 1, No. 4, p:115-7.
  • Bulduk E,Ö.[2010] Bayan sporcularda 20 metre mekik koşu testinin oksidatif stres ve antioksidan düzeyleri üzerine etkisinin incelenmesi, Selçuk University, doctoral dissertation.
  • Clarkson PM, Thompson HS.[2000] Antioxidants: what role do they play in physical activity and health?. Am J Clin Nutr.72(2 Suppl):637S-46S.
  • Clarkson PM.[1995] Antioxidants and physical performance. Crit Rev Food Sci Nutr .35:131–141.
  • Demir CY, Kocak OF, Bozan N, Ersoz ME, Demir H. [2017] Is There a Role for Oxidative Stress in Temporomandibular Joint Disorders? J Oral Maxillofac Surg. 21. pii: S0278-2391(17)31422-2. doi: 10.1016/j.joms.2017.11.004. [Epub ahead of print].
  • Ersoy G, Hasbay A.[1998] Egzersize bağlı serbest radikal oluşumu ve serbest radikal temizleyicisi besin ögeleri. Spor ve Tıp. Yıl 6: 11-12.
  • Gecit İ, Eryılmaz R, Kavak S, Meral İ, Demir H, Pirinççi N, Güneş M,[2017] Taken K. The Prolidase Activity, Oxidative Stress, and Nitric Oxide Levels of Bladder Tissues with or Without Tumor in Patients with Bladder Cancer. J Membr Biol. 2017 Oct;250(5):455-459. doi: 10.1007/s00232-017-9971-0. Epub .16.
  • Gutteridge, J.M., [1995] Lipit peroxidation and antioxidants as biomarkers of tissue damage, Clin. Chem., 41 (12): 1819–1828.
  • Hambrecht R, Wolf A, Gielen S, et al.[2000] Effect of exercise on coronary endothelial function in patients with coronary artery disease. N Engl J Med.342:454-460.
  • Holmes RS and Masters CJ[1972] Species specific feature of the distribution and multiplicity of mammalian liver catalases.Arch.Biochem. Biophys., 148,217-233.
  • Memişoğulları R [2005] Diyabette serbest radikallerin rolü ve antioksidanların etkisi. Duzce Medical Journal;3:30-39.
  • Pani G, Colavitti R, Bedogni B, et al [2000] A redox signaling mechanism for density-dependent inhibition of cell growth. J Biol Chem;275:38891–38899.
  • Percy ME [1984] Catalase an old enzyme with a new role?, Can.J.Biochem.Cell Biol.62: 1006- 1014.
  • Radak Z, Chung HY, Goto S.[2008] Systemic adaptation to oxidative challenge induced by regular exercise. Free Radic Biol Med. 44(2):153-9.
  • Sachdev S, Davies KJ.[2008] Production, detection, and adaptive responses to free radicals in exercise. Free Radic Biol Med. 15;44(2):215-23.
  • Sies H. [1997] Oxidative stress: oxidants and antioxidants. Exp Physiol.82:292-295.
  • Sureda A, Tauler P, Aguiló A, Cases N, Fuentespina E, Córdova A, Tur JA, Pons A.[2005] Relation between oxidative stress markers and antioxidant endogenous defences during exhaustive exercise. Free Radic Res. 39(12):1317-24.
  • Urso ML, Clarkson PM [2003] Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 15;189(1-2):41-54.
  • Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. [2006] Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 10;160(1):1-40.
  • Wellman KF, Bloomer RJ.[2009] Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine. 13;8:1, 1-5.
Year 2019, Volume: 8 Issue: 4, 1296 - 1301, 24.12.2019
https://doi.org/10.17798/bitlisfen.642861

Abstract

References

  • Aebi H [1984] Catalase.(in) Methots In Enzymology, L. Packer(Editör), 105: 121-126, Academic Pres, Orlando.
  • Aguiló A, Tauler P, Gimeno I, Fuentespina E, Pons A.[2000] Changes in erythrocyte antioxidant enzymes during prolonged submaximal exercise. Biofactors.11(1-2):27-30.
  • Akkuş İ. [1995] Serbest Radikaller ve Fizyopatolojik Etkileri(Free Radicals and Their Physiopathologic Effects). Baskı 1. Konya, Mimoza Basım – Yayım ve Dağıtım A.Ş, a. 49-50.
  • Alptekin Ö, Yıldırım D, Özyılmaz G ve Tokel S [2004] Florosile immobilize edilmiş katalaz enziminin immobilizasyon koşullarının optimizasyonu, XVIII. National Chemistry Congree, BK, S. 498, Kars
  • Alvying A, Carson P, Flanagan CL, Ickes C, [1956] Enzymatic deficiency in primaquin-sensitive erythrocytes. Science, 14, (124):484-5.
  • Arslan A, Tüzün FA, Arslan H, Demir H, Tamer S, Demir C, Tasin M. [2016] The relationship between serum paraoxonase levels and carotid atherosclerotic plaque formation in Alzheimer's patients. Neurol Neurochir Pol. Dec;50(6):403-409.
  • Asker S, Asker M, Sarikaya E, Sunnetcioglu A, Aslan M, Demir H.[2015] Oxidative stress parameters and their correlation with clinical, metabolic and polysomnographic parameters in severe obstructive sleep apnea syndrome. Int J Clin Exp Med. 2015 Jul 15;8(7):11449-55.
  • Aslan R, Şekeroğlu MR.[1996] Egzersize bağlı lipid peroksidasyonu ve antioksidan statüsü çalışmalarında sonuçlara etkili faktörler. Turkish Journal of Sports Medicine, 31: 145-152.
  • Atli M. [2013] Serum paraoxonase activity and lipid hydroperoxide levels in adult football players after three days football tournament, African Health Sciences Vol 13 Issue 3 September
  • Banerjee AK, Mandal A, Chanda D, Chakraborti S.[2003] Oxidant, antioxidant and physical exercise. Mol Cell Biochem.253(1-2):307-12.
  • Beutler E., Duron O., Kelly BM., [1963] Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882‐888
  • Bilici M, Cim N, Demir H. [2014] Pre and post-operative oxidative stress level in cases with ovarian cancer neoplasia. Medical Science and Discovery, Vol. 1, No. 4, p:115-7.
  • Bulduk E,Ö.[2010] Bayan sporcularda 20 metre mekik koşu testinin oksidatif stres ve antioksidan düzeyleri üzerine etkisinin incelenmesi, Selçuk University, doctoral dissertation.
  • Clarkson PM, Thompson HS.[2000] Antioxidants: what role do they play in physical activity and health?. Am J Clin Nutr.72(2 Suppl):637S-46S.
  • Clarkson PM.[1995] Antioxidants and physical performance. Crit Rev Food Sci Nutr .35:131–141.
  • Demir CY, Kocak OF, Bozan N, Ersoz ME, Demir H. [2017] Is There a Role for Oxidative Stress in Temporomandibular Joint Disorders? J Oral Maxillofac Surg. 21. pii: S0278-2391(17)31422-2. doi: 10.1016/j.joms.2017.11.004. [Epub ahead of print].
  • Ersoy G, Hasbay A.[1998] Egzersize bağlı serbest radikal oluşumu ve serbest radikal temizleyicisi besin ögeleri. Spor ve Tıp. Yıl 6: 11-12.
  • Gecit İ, Eryılmaz R, Kavak S, Meral İ, Demir H, Pirinççi N, Güneş M,[2017] Taken K. The Prolidase Activity, Oxidative Stress, and Nitric Oxide Levels of Bladder Tissues with or Without Tumor in Patients with Bladder Cancer. J Membr Biol. 2017 Oct;250(5):455-459. doi: 10.1007/s00232-017-9971-0. Epub .16.
  • Gutteridge, J.M., [1995] Lipit peroxidation and antioxidants as biomarkers of tissue damage, Clin. Chem., 41 (12): 1819–1828.
  • Hambrecht R, Wolf A, Gielen S, et al.[2000] Effect of exercise on coronary endothelial function in patients with coronary artery disease. N Engl J Med.342:454-460.
  • Holmes RS and Masters CJ[1972] Species specific feature of the distribution and multiplicity of mammalian liver catalases.Arch.Biochem. Biophys., 148,217-233.
  • Memişoğulları R [2005] Diyabette serbest radikallerin rolü ve antioksidanların etkisi. Duzce Medical Journal;3:30-39.
  • Pani G, Colavitti R, Bedogni B, et al [2000] A redox signaling mechanism for density-dependent inhibition of cell growth. J Biol Chem;275:38891–38899.
  • Percy ME [1984] Catalase an old enzyme with a new role?, Can.J.Biochem.Cell Biol.62: 1006- 1014.
  • Radak Z, Chung HY, Goto S.[2008] Systemic adaptation to oxidative challenge induced by regular exercise. Free Radic Biol Med. 44(2):153-9.
  • Sachdev S, Davies KJ.[2008] Production, detection, and adaptive responses to free radicals in exercise. Free Radic Biol Med. 15;44(2):215-23.
  • Sies H. [1997] Oxidative stress: oxidants and antioxidants. Exp Physiol.82:292-295.
  • Sureda A, Tauler P, Aguiló A, Cases N, Fuentespina E, Córdova A, Tur JA, Pons A.[2005] Relation between oxidative stress markers and antioxidant endogenous defences during exhaustive exercise. Free Radic Res. 39(12):1317-24.
  • Urso ML, Clarkson PM [2003] Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 15;189(1-2):41-54.
  • Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. [2006] Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 10;160(1):1-40.
  • Wellman KF, Bloomer RJ.[2009] Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine. 13;8:1, 1-5.
There are 31 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Araştırma Makalesi
Authors

Mustafa Atlı 0000-0002-3868-4570

Mustafa Bilici 0000-0002-8689-6463

Publication Date December 24, 2019
Submission Date November 5, 2019
Acceptance Date November 27, 2019
Published in Issue Year 2019 Volume: 8 Issue: 4

Cite

IEEE M. Atlı and M. Bilici, “Determination of Degrees of Oxidative Stress and Some Antioxidant Enzyme Activities in Basketball Players”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 8, no. 4, pp. 1296–1301, 2019, doi: 10.17798/bitlisfen.642861.

Bitlis Eren University
Journal of Science Editor
Bitlis Eren University Graduate Institute
Bes Minare Mah. Ahmet Eren Bulvari, Merkez Kampus, 13000 BITLIS