A new approach for prevention the oxidations and mutations: Zinc borate

Year 2019, Volume: 4 Issue: 4, 196 - 202, 31.12.2019

Aysel Ugur , Ozgur Ceylan , Rukiye Boran , Semih Ayrikcil Nurdan Sarac , Dervis Yilmaz

https://doi.org/10.30728/boron.573718

Cited By: 4

Abstract

Zinc
borate is a white crystalline powder
substance with variable composition (34% B₂O₃, 45% ZnO
and 20% H₂O). It is used as a fungus and mildew inhibitor, to fire
proof textiles, and for other uses. Although there are limited studies about
the biologic properties of this compound. The present study was aimed to
investigate the antioxidant and antimutagenic activity of zinc borate and also
to figure out its cytotoxic effect. Antioxidant activity of zinc borate was
determined with β- carotene linoleic acid and DPPH radical scavenging assays.
The mutagenicity and antimutagenic activity was determined with AMES/ Salmonella microsomal test systems using
Salmonella typhimurium TA98 and TA100
strains. The cytotoxic effect of zinc borate was evaluated by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay using
mouse fibroblast L929 cell line. When compared to the control, % inhibition
values of 1 mg/mL zinc borate for DPPH radical scavenging and β- carotene
linoleic acid tests were calculated as 13.50% and 30%, respectively. At the
tested concentrations, zinc borate did not exhibit any mutagenic effect. Zinc
borate exhibited low antimutagenic activity at 0.2 and 0.04 mg/plate
concentrations on S. typhimurium TA98
strain while it exhibited no antimutagenic activity on S. typhimurium TA100 strain. In
vitro toxicity against L929 cell line with IC₅₀ values of zinc
borate were found to be 40.70 µg/mL and 32.93 µg/mL for 24h and 72h,
respectively. In spite of the low DPPH free radical scavenging activity, zinc
borate has found to have moderate total antioxidant activity besides low
antimutagenic potential. Zinc borate has a potential to use for medical purpose
with its antioxidative and antimutagenic properties.

Keywords

Zinc borate, antioxidant, antimutagenic, Cytotoxic effect

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