SERULEİNLE OLUŞTURULMUŞ SIÇAN AKUT PANKREATİT MODELINDE BETA GLUKAN VE FUKOKSANTİNİN ETKİSİ The Effect of Beta Glucan and Fucoxanthine in a Serulein- Induced Acute Pancreatitis in Rats

Year 2020, Volume: 10 Issue: 2, 189 - 197, 08.06.2020

Dilek Özbeyli Özlem Tuğçe Çilingir Kaya Aslı Aykaç Sezgin Aydemir Esra Bihter Gürler Meral Yüksel

Abstract

ÖZET
Amaç: Bu çalışmada, Beta Glukan, fukoksantin ve kombinasyonlarının serulein kaynaklı akut pankreatit (AP)
sıçan modelindeki etkileri araştırıldı.
Gereç ve Yöntem: Sıçanlar kontrol ve AP gruplarına ayrıldı. AP bir saat arayla dört kez serulein (20 ug/kg/
ip) enjeksiyonuyla oluşturuldu. Serulein enjeksiyonundan 3 gün önce AP gruplarına günde 1 kez taşıyıcı,
Beta Glukan (BG;40 mg/kg/po), fukoksantin (Fuko;40 mg/kg/po) veya Beta Glukan (BG;40 mg/kg/po)
+fukoksantin (Fuko;40 mg/kg/po) uygulandı. Son enjeksiyondan sonraki 8. saatte ötenazi gerçekleştirildi.
Serumda amilaz ve lipaz, pankreas dokusunda interlökin-1β (IL-1β), interlökin-6 (IL-6), interlökin-10 (IL-
10), lucigenin kemilüminesans (CL), malondialdehit (MDA), miyeloperoksidaz (MPO) enzim aktivitesi ve
Hematoksilen&Eosin (H&E), Siklooksijenaz-2 (COX-2), nükleer faktör kappa B (NF-κB) histopatolojik incelemeleri
yapıldı. İstatistiksel analizler için tek yönlü ANOVA ve Bonferroni post-hoc testi uygulandı.
Bulgular: Taşıyıcı grubunda; amilaz, lipaz, lusigenin, IL-1β ve IL-6 düzeyleri kontrole göre (p <0,01-0,0001)
yükselirken, GSH ve IL-10 düzeyleri azaldı (p<0,0001). Lipaz ve amilaz düzeyleri; BG, Fuko ve BG+Fuko tedavileri
ile taşıyıcıya göre azaldı (p<0,05-0,001). IL-6 düzeyleri, Fuko ve BG+Fuko grubunda taşıyıcıya göre
azaldı (p <0,01). IL-1β düzeyleri; BG, Fuko ve BG+Fuko gruplarında taşıyıcı grubuna göre azaldı (p<0,001-
0.0001). IL-10 düzeyi yalnızca BG grubunda taşıyıcı grubuna göre arttı (p<0,01). H&E, COX-2 skorları taşıyıcı
grubunda kontrole göre artarken (p<0,0001); BG, Fuko ve BG+Fuko tedavileri ile azalma gözlendi (p<0,05-
0,001). Taşıyıcı grubunda artan NF-κB skorları (p<0,0001), BG tedavisi ile azaldı (p<0,01). Taşıyıcı grubundaki
artan lusigenin kemiluminesans, MPO ve MDA düzeyleri (p <0,01-0,0001) tüm tedavilerle azaldı (p <0,01-
0,0001).
Sonuç: Beta Glukan ve fukoksantin tedavisi pro-inflamatuvar sitokinleri, COX-2 düzeyini ve oksidatif belirteçleri
azaltarak anti-inflamatuvar sitokin düzeyini arttırarak AP gelişimini hafifletmiştir.
Anahtar sözcükler: Beta glukan; fukoksantin; COX-2; oksidatif stres
ABSTRACT
Aim: This study aims to investigate the effects of Beta Glucan, fucoxanthin and their combinations in acute
pancreatitis (AP) rat model.
Material and Methods: Rats were divided into control and AP groups. The AP was induced by injection of
cerulein (20 μg/kg/ip) four times with an hour interval. Vehicle, Beta Glucan (BG;40 mg/kg/po), fucoxanthin
(Fuco;40 mg/kg/po) or Beta Glucan (BG;40 mg/kg/+ fucoxanthin (Fuco;40 mg/kg/po) were administered
3 days before cerulein injection. Euthanasia was performed at the 8th hour after the last injection. Serum
and pancreatic tissue collected for biochemical and histopathological examinations. One-way ANOVA and
Bonferroni post-hoc test was used for statistical analysis.
Results: Amylase, lipase, lucigenin, interleukin-1β, and interleukin-6 levels are significantly elevated in the
vehicle-treated AP groups (p<0.01-0.0001) while IL-10 was decreased (p<0.0001). Lipase and amylase levels
decreased in the BG, Fuco and BG+Fuco groups compared to vehicle group (p<0.05-0.001). IL-6 levels,
decreased in the fuco and BG+Fuco group (p <0.01). IL-1β levels decreased in BG, Fuco and BG+Fuco groups
compared to vehicle group (p<0,001-0.0001). IL-10 levels increased in BG group compared to the vehicle
group (p<0.01). H&E and COX-2 scores increased in the vehicle groups, compared to the control group
(p<0.0001). All treatments decreased that scores (p<0.05-0.001). Increased NF-κB scores with vehicle
(p<0.0001), decreased with BG treatment (p<0.01). Increased lucigenin, MPO and MDA levels (p<0.01-
0.0001), decreased with BG, Fuco and BG+Fuco treatments (p<0.01-0.0001).
Conclusion: Treatment of Beta Glucan and fucoxanthin alleviated AP development by decreasing proinflammatory
cytokines, COX-2 and oxidative markers and increasing anti-inflammatory cytokine.
Keywords: Beta glucan; fucoxanthine; COX-2; Oxidative stress

Keywords

SERULEİNLE OLUŞTURULMUŞ SIÇAN AKUT PANKREATİT MODELINDE BETA GLUKAN VE FUKOKSANTİNİN ETKİSİ The Effect of Beta Glucan and Fucoxanthine in a Serulein- Induced Acute Pancreatitis in Rats

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