Investigation of Mycoplasma bovis and Ureaplasma diversum from Bovine Aborted Fetuses in Northeast Anatolia Region by PCR

Year 2020, Volume: 1 Issue: 1-2, 13 - 16, 15.12.2020

Ediz Kağan Özgen Berna Yanmaz Mustafa Ulucan Perihan Şerifoğlu Bağatır Murat Özmen Elif Karadeniz Pütür

Abstract

Abortion is one of the most important problems in animal husbandry due to its hindrance in obtaining offspring, labor, and economic losses. Although Mycoplasma bovis (M. bovis) and Ureaplasma diversum (U. diversum) are the bacteria found in the genital flora, they are considered as important pathogens that cause bacterial abortion. The aim of this study was to investigate the presence of M. bovis and U. diversum in aborted cattle fetuses from Northeastern Anatolia province by PCR method. In this study, a total of 187 cattle fetuses samples (lung and liver tissues and abomasum content of fetuses) were examined from March to June 2019. The fetuses samples were taken from the provinces of Ağrı, Ardahan, Artvin, Erzincan, Erzurum, Gümüşhane, Iğdır, and Kars. M. bovis was positive in 4.8% (9/187) and U. diversum was positive in 2.7% (5/187) of the fetus samples. As a result, both agents should be considered in routine investigations when investigating the infectious causes of abortion cases. Further studies may be beneficial to determine the pathogenicity and type strains of M. bovis and U. diversum in aborted cattle fetuses.

Keywords

Abortion, Cattle, Mycoplasma bovis, Ureaplasma diversum

References

• 1. Reichel MP, Wahl LC, Hill FI. Review of diagnostic procedures and approaches to infectious causes of reproductive failures of cattle in Australia and New Zealand. Frontiers in Veterinary Science 2018; 5: 222.
• 2. Hermeyer K, Peters M, Brügmann M, Jacobsen B, Hewicker-Trautwein M. Demonstration of Mycoplasma bovis by immunohistochemistry and in situ hybridization in an aborted bovine fetus and neonatal calf. Journal of Veterinary Diagnostic Investigation 2012; 24: 364–369.
• 3. Bürki S, Frey J, Pilo P. Virulence, persistence and dissemination of Mycoplasma bovis. Veterinary Microbiology 2015; 179: 15–22.
• 4. Parker AM, Sheehy PA, Hazelton MS, Bosward KL, House JK. A review of Mycoplasma diagnostics in cattle. Journal of Veterinary Internal Medicine 2018; 32: 1241–1252.
• 5. Ball HJ, Neill SD, Ellis WA, O’Brien JJ, Ferguson HW. The isolation of Mycoplasma from bovine foetuses and their dams. British Veterinary Journal 1978; 134: 584–589.
• 6. Dando SJ, Sweeney EL, Knox CL. Ureaplasma. Bergey's Manual of Systematics of Archaea and Bacteria, 2015; 1-28.
• 7. Parkinson TJ. Specific Infectious Diseases Causing Infertility and Subfertility in Cattle. In “Veterinary Reproduction and Obstetrics” Ten Edition. Elsevier BV, Edinburgh, Scotland, 2019; pp.434-466. 8. Diaz JM, Prieto A, Lopez G, Diaz P, Lopez C, et al. Association of Ureaplasma diversum with reproductive disease in cattle. New Zealand Veterinary Journal 2019; 67: 249–256.
• 9. Songer JG, Post KW. Veterinary Microbiology. Bacterial and Fungal Agents of Animal Disease. St Louis: Elsevier Saunders, 2005; pp.84–91.
• 10. Tramuta C, Lacerenza D, Zoppi S, Goria M, Dondo A, et al. Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples. Journal of Veterinary Diagnostic Investigation 2011; 23: 657–664.
• 11. Wolf-Jäckel GA, Hansen MS, Larsen G, Holm E, Agerholm JS, et al. Diagnostic studies of abortion in Danish cattle 2015–2017. Acta Veterinaria Scandinavica 2020; 62: 1.
• 12. Reichel MP, Wahl LC, Hill FI. Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand. Frontiers in Veterinary Science 2018; 5: 222.
• 13. Pfützner H, Sachse K. Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Revue Scientifique Technique 1996; 15: 1477–1494.
• 14. Akan M, Babacan O, Torun E, Müştak HK, Öncel T. Diagnosis of Mycoplasma bovis infection in cattle by ELISA and PCR. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2014; 20: 249-252.
• 15. Byrne WJ, Brennan P, McCormack R, Ball HJ. Isolation of Mycoplasma bovis from the abomasal contents of an aborted bovine fetus. Veterinary Record 1999; 144: 211–212.
• 16. Kumar A, Verna AK, Rahal A. Mycoplasma bovis, a multi disease producing pathogen: An overview. Asian Journal of Animal and Veterinary Advances 2011; 6: 537-546.
• 17. Syrjala P, Anttila M, Dillard K, Fossi M, Collin K et al. Causes of bovine abortion, stillbirth and neonatal death in Finland 1999–2006. Acta Veterinaria Scandinavica 2007; 49: 3.
• 18. Trichard CJ, Jacobs EP. Mycoplasmas recovered from bovine genitalia, aborted foetuses and placentas in the Republic of South Africa. Onderstepoort Journal of Veterinary Research 1985; 52: 105–110.
• 19. Cardoso MV, Blanchard A, Ferris S, Verlengia R, Timenetsky J, et al. Detection of Ureaplasma diversum in cattle using a newly developed PCR-based detection assay. Veterinary Microbiology 2000; 72: 241–250.