ISI ŞOK PROTEİNLERİ VE İN VİTRO EMBRİYO

Year 2018, Volume: 1 Issue: 3, 89 - 93, 01.07.2018

Leyla Bener , Rabia Yaşar Mehmet Kuran

Abstract

Uzun yıllardır çeşitli şekillerde in vitro embriyo üretimi yapılmaktadır. Bu alanda önemli bir mesafe alınmasına rağmen istenilen seviyede in vitro embriyo üretiminin gerçekleştirilememesinin nedeni in vitro kültür ortamların da in vivo şartların tam olarak taklit edilememesidir. İn vitro kültür ortamlarında bir takım biyolojik, çevresel bileşen ve ortam sıcaklığında embriyonun strese maruz kalıp tepki olarak stres proteinleri üretmektedir. Stres proteinleri, organizmanın maruz kaldığı kültür medyumu bileşenleri, serum (FSC, ECS), UV ışınları, toksin, arsenik, pH değişimi, iskemia, oksidatif fosforilasyon, sıcak-soğuk ve enfeksiyon durumunda yanıt olarak üretimi artan ve büyüklükleri 100-8 kDA arasında değişen proteinlerdir. Bu proteinlerden HSP70, yüksek oranda korunmuş olan kompakt amino termal bölgesi ve zayıf ATPaz aktivitesi gibi iki büyük etki alanına sahiptir ve ısı şok faktörlerin (HSF) aktivitesini düzenler. İn vitro kültürde embriyo gelişimi çevresel streslerden etkilenmekte ve embriyo gelişim sürecinde HSP70’in apoptoziz de rol aldığına ilişkin deliller bulunmaktadır. Kültür ortamında serum varlığının embriyo implantasyon oranını düşürebileceği düşünülmektedir. Bu derlemede, in vitro oosit olgunlaştırması ve embriyo üretiminde strese bağlı olarak üretilen ısı şok proteinleri ve bunların çeşitleri açıklanmaya çalışılmıştır. Ayrıca bu proteinlerin in vitro embriyo üretim etkinliğine olan muhtemel etkileri de tartışılmıştır.

Keywords

Embriyo, Kültür medyumları, Isı şok proteinleri, Apoptoziz

Heat Shock Proteins and In Vitro Embryo

Abstract

For
a long period of time significant progress attained in in vitro embryo
production. Despite desired levels has not been reached  the main reason are inability to imitate in
vivo conditions of in vitro culture medium precisely and embryo be exposed to
stress factors such as environmental conditions, temperature and biological
components as a reaction produce stress proteins. Stress proteins which are
increased in the organism during culture medium components, serums (FCS, ECS),
UV rays, toxin, arsenic, pH, ischemia, oxidative phosphorylation, cold and hot
conditions and during infections and vary in size between 100 to 8 kDA. HSP70
has two main domains, compact amino terminal site and low ATPase activity which
been highly conserved and it regulate heat shock activity. During in vitro
embryo production, the effect of environmental stress and embryo development
processes in relation with the roll of HSP70 is very important. Presence of
serum in the culture medium thought that it decreased the embryo development rate.
In this review, we attempted to explain heat shock proteins and their varieties
produced in vitro by oocyte maturation and embryo production. Furthermore, the
possible effects of these proteins on in vitro embryo production activity have
been discussed.

Keywords

Embryo, Culture medium, Heat shock, Proteins, Apoptosis

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